Date published: 2025-9-7
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CD9 Antibody (P1/33/2) - Western Blotting - Image 109841
CD9 抗体 (P1/33/2): sc-20048. 对 HeLa 全细胞裂解液中 CD9 的表达进行 Western 印迹分析.
CD9 抗体 (P1/33/2): sc-20048. 对 HeLa 全细胞裂解液中 CD9 的表达进行 Western 印迹分析.

CD9 抗体 (P1/33/2): sc-20048

4.7(6)
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说明书
  • CD9抗体(P1/33/2)是小鼠单克隆IgG1 κ, 在17篇文献中引用，规格为200 µg/ml
  • 针对常见急性淋巴细胞白血病
  • 推荐用于 mouse, rat 和 human 来源的CD9 WB, IP 和 IF检测
  • 看 CD9 (C-4): sc-13118，可查看有标记的 CD9 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是CD9 Antibody (P1/33/2) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与CD9 Antibody (P1/33/2) 打包提供（请参阅下面的订购信息）。

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描述
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研究信息

関連項目

此外 CD9 抗体包括 CD9P-1

CD9抗体（P1/33/2）是一种小鼠单克隆IgG1 kappa轻链抗体，针对常见的急性淋巴细胞白血病抗原，可与小鼠、大鼠和人类反应，并经过验证可用于包括蛋白质印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）在内的多种应用。CD9，也称为Tetraspanin-29，是一种IV型跨膜糖蛋白，具有四个跨膜结构域，主要位于各种细胞类型的质膜上，包括早期B细胞、嗜酸性粒细胞、嗜碱性粒细胞、活化T细胞和血小板。CD9（P1/33/2）抗体在细胞表面的定位对于调节细胞间粘附和促进相邻细胞之间的相互作用至关重要，而细胞间粘附和相互作用对于免疫反应、组织修复和细胞信号传导至关重要。CD9单克隆抗体（P1/33/2）还通过与低分子量GTP结合蛋白相互作用参与信号传导通路，影响细胞内迁移、增殖和粘附等过程。CD9是血小板表面的重要组成部分，在血小板聚集和止血中起着关键作用。此外，CD9在各种血液系统恶性肿瘤中均有表达，包括大多数非T急性淋巴细胞白血病细胞和一些急性髓细胞白血病和慢性淋巴细胞白血病，这使得CD9（P1/33/2）单克隆抗体成为诊断和研究的重要标志物。抗CD9抗体（P1/33/2）为研究这种重要的跨膜蛋白的功能、细胞定位和病理作用提供了多功能工具，并且以非结合形式提供，适用于各种实验应用。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

CD9 抗体 (P1/33/2)

sc-20048
200 µg/ml
RMB2377.00

CD9 (P1/33/2): m-IgG Fc BP-HRP 套装

sc-536645
200 µg Ab; 10 µg BP
RMB2662.00

CD9 (P1/33/2): m-IgGκ BP-HRP 套装

sc-533803
200 µg Ab; 40 µg BP
RMB2662.00

CD9 (P1/33/2): m-IgG1 BP-HRP 套装

sc-544729
200 µg Ab; 20 µg BP
RMB2662.00

Is this antibody conformation dependent? When using for western blot to detect the presence of extracellular vesicles in a sample, should it be used with non-reducing sample buffer?

Asked by: Victoria
Thank you for contacting Santa Cruz Biotechnology. For assistance please contact Technical service. Please call 800-457-3801 or email [email protected].
Answered by: BlakeJ
Date published: 2025-04-11

Hello, I’m reaching out to ask whether the CD9 antibody (P1/33/2), catalog number sc-20048, is conformation-dependent. Specifically, when using this antibody for Western blot, should reducing agents be avoided to preserve epitope recognition? Thank you

Asked by: LiLian
Thank you for your question. sc-20048 CD9 (P1/33/2) should be used under reducing conditions. https://www.scbt.com/resources/protocols/western-immuno-blotting
Answered by: BlakeJ
Date published: 2025-04-04

What is the recommended fixation method for immunofluorescence staining with CD9 (P1/33/2): sc-20048 monoclonal antibody?

Asked by: cjMara
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Works well for IF!Worked really well for IF! We're happy with it, it's much better than our previous antibody. We haven't tried it for other types of assays yet.
Date published: 2021-05-11
Rated 5 out of 5 by from Good performanceGood performance for WB, specific band at 24kda (Hela cell positive control),we suggest not expeosed too long,we have tried different exposed time , too long may caused very high background
Date published: 2017-09-10
Rated 5 out of 5 by from Publishable IHC data obtained in human ABCG2+Publishable IHC data obtained in human ABCG2+ IGR37 cells xenografts -SCBT Publication Review
Date published: 2015-04-15
Rated 4 out of 5 by from Very large WB band obtained using human PBLVery large WB band obtained using human PBL whole cell lysate. -SCBT QC
Date published: 2015-02-10
Rated 5 out of 5 by from Great immunofluorescence cytoplasmic stainingGreat immunofluorescence cytoplasmic staining in methanol-fixed Hela cells. -SCBT QC
Date published: 2015-01-31
Rated 4 out of 5 by from Decent WB signal achieved in HeLa whole cellDecent WB signal achieved in HeLa whole cell lysate. -SCBT QC
Date published: 2015-01-29
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CD9 抗体 (P1/33/2) is rated 4.7 out of 5 by 6.
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