Date published: 2025-9-7
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CD81 Antibody (D-4) - Western Blotting - Image 380203
CD81 抗体 (D-4): sc-166028. 对 JAR (A), Jurkat (B), SUP-T1 (C) 和 BJAB (D) 全细胞裂解液及大鼠胸腺组织提取物 (E) 中 CD81 表达的 Western 印迹分析.
CD81 抗体 (D-4): sc-166028. 对 JAR (A), Jurkat (B), SUP-T1 (C) 和 BJAB (D) 全细胞裂解液及大鼠胸腺组织提取物 (E) 中 CD81 表达的 Western 印迹分析.

CD81 抗体 (D-4): sc-166028

4.8(12)
寫評論提問

说明书
  • CD81抗体(D-4)是小鼠单克隆IgG2b κ, 在33篇文献中引用，规格为200 µg/ml
  • 免疫human物种的CD81的氨基酸90-210
  • 抗-CD81 抗体 (D-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 CD81
  • 抗CD81抗体（D-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD81 (D-4): sc-166028的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 CD81 抗体 (D-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD81 抗体 (D-4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

CD81抗体（D-4）是一种IgG2b κ小鼠单克隆CD81抗体（也称为CD81抗体或TAPA1抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的CD81。CD81抗体（D-4）既可以作为非偶联的抗CD81抗体形式，也可以作为多种偶联形式的抗CD81抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。CD81，也称为TAPA-1，是一种III型跨膜蛋白，在造血、神经外胚层和间充质来细胞中广泛表达。人们认为CD81既参与细胞生长也参与信号转导。它可以作为与CD37和/或CD53相关的多分子复合物存在，或存在于与CD19、CD21和/或MHC II类抗原相关的B细胞表面。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CD81 抗体 (D-4)

sc-166028
200 µg/ml
RMB2377.00

CD81 (D-4): m-IgGκ BP-HRP 套装

sc-521488
200 µg Ab, 40 µg BP
RMB2662.00

CD81 (D-4): m-IgG2b BP-HRP 套装

sc-548912
200 µg Ab; 10 µg BP
RMB2662.00

CD81 抗体 (D-4) AC

sc-166028 AC
500 µg/ml, 25% agarose
RMB3129.00

CD81 抗体 (D-4) HRP

sc-166028 HRP
200 µg/ml
RMB2377.00

CD81 抗体 (D-4) FITC

sc-166028 FITC
200 µg/ml
RMB2482.00

CD81 抗体 (D-4) PE

sc-166028 PE
200 µg/ml
RMB2580.00

CD81 抗体 (D-4) Alexa Fluor® 488

sc-166028 AF488
200 µg/ml
RMB2685.00

CD81 抗体 (D-4) Alexa Fluor® 546

sc-166028 AF546
200 µg/ml
RMB2685.00

CD81 抗体 (D-4) Alexa Fluor® 594

sc-166028 AF594
200 µg/ml
RMB2685.00

CD81 抗体 (D-4) Alexa Fluor® 647

sc-166028 AF647
200 µg/ml
RMB2685.00

CD81 抗体 (D-4) Alexa Fluor® 680

sc-166028 AF680
200 µg/ml
RMB2685.00

CD81 抗体 (D-4) Alexa Fluor® 790

sc-166028 AF790
200 µg/ml
RMB2685.00

CD81 antibody (D-4):sc-166028についておたずねです。western blottingは還元剤が必要でしょうか、不要でしょうか。

Asked by: Anonymous
Thank you for your question. western blotting does not require a reducing agent. Thank you very much. 質問ありがとうございます。western blottingは還元剤が不要となります。よろしくお願いします。
Answered by: Jiajun Z
Date published: 2025-02-26

Will this antibody detect CD81 if the lysates contain DTT?  Some anti-CD81 antibodies could only detect it when the lysates are not reduced. 

Asked by: yanwu
Thank you for your question. The Western blot data shown on our website for this antibody, CD81 (D-4): sc-166028, is under reducing conditions. A link to our recommended protocol is provided below as a reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2024-11-15

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue? Or even double or triple staining on the same samples?

Asked by: DefinitelyNotMatt
Thank you for your question. No. We recommend using a directly conjugated mouse monoclonal primary antibody, SC-166029. This antibody is currently available conjugated to phycoerythrin (sc-166029 PE), fluorescein (sc-166029 FITC), Alexa Fluor® 488 (sc-166029 AF488) or Alexa Fluor® 647 (sc-166029 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 12
Date published: 2017-01-11
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Rated 4 out of 5 by from good antibody for WBI tested it on WB using both exosome and fibroblast lysates. It is perfect for exosome and OK for fibroblast lysate.
Date published: 2017-08-18
Rated 5 out of 5 by from specific bandUse the antibody dilution 1:1000 for WB. Nice single band at around 23 kDa ( rat spleen tissue ).
Date published: 2017-07-23
Rated 3 out of 5 by from Works for mouse brain sampleWorks for mouse brain lysate homogenate sample and in vivo mouse exosome isolated samples
Date published: 2017-07-20
Rated 5 out of 5 by from target band seenAfter expose the target band is very clean and the Mw is right,recommnd for human breast cancer cell detection.
Date published: 2017-06-25
Rated 5 out of 5 by from IF stainingUsed it in IF staining human smooth muscle tissue and worked at dilution 1:200, It gave a clear membrane staining.
Date published: 2017-02-07
Rated 5 out of 5 by from Superb !!Very strong product both for IHC and western applications
Date published: 2017-01-07
Rated 5 out of 5 by from Western blot good resultExcellent WB results observed in human cell lines. Bands look great.
Date published: 2016-12-23
Rated 5 out of 5 by from WB and TEM on mouse C2C12 myoblasts with scWB and TEM on mouse C2C12 myoblasts with sc-166028 (1/1000 in 1% BSA) give very good results - Published data
Date published: 2016-05-06
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CD81 抗体 (D-4) is rated 4.8 out of 5 by 12.
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