Date published: 2025-9-7
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CD81 Antibody (B-11) - Western Blotting - Image 391191
CD81 抗体 (B-11) Alexa Fluor® 488: sc-166029 AF488. U-698-M (A) 和 BJAB (B) 全细胞裂解液中 CD81 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214. 用 Cruz Marker MW Tag-Alexa Fluor® 680 检测 Cruz Marker™ 分子量标准: sc-516730.
CD81 抗体 (B-11) Alexa Fluor® 488: sc-166029 AF488. U-698-M (A) 和 BJAB (B) 全细胞裂解液中 CD81 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214. 用 Cruz Marker MW Tag-Alexa Fluor® 680 检测 Cruz Marker™ 分子量标准: sc-516730.

CD81 抗体 (B-11): sc-166029

4.6(30)
寫評論提問

说明书
  • CD81 抗体 B-11 是小鼠单克隆 IgG2b κ，CD81抗体, 在391篇文献中引用，规格为200 µg/ml
  • 免疫human物种的CD81的氨基酸90-210
  • CD81 抗体 (B-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 CD81
  • 抗CD81抗体（B-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD81 (B-11): sc-166029的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 CD81 抗体 (B-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD81 抗体 (B-11) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

CD81抗体（B-11）是一种IgG2b κ小鼠单克隆CD81抗体（也称为TAPA1抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的CD81蛋白。CD81抗体（B-11）既可以作为非偶联的抗CD81抗体形式，也可以作为多种偶联形式的抗CD81抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。CD81，也称为TAPA-1，是一种III型跨膜蛋白，在造血、神经外胚层和间充质细胞中广泛表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CD81 抗体 (B-11)

sc-166029
200 µg/ml
RMB2377.00

CD81 (B-11): m-IgGκ BP-HRP 套装

sc-521489
200 µg Ab, 40 µg BP
RMB2662.00

CD81 (B-11): m-IgG2b BP-HRP 套装

sc-548913
200 µg Ab; 10 µg BP
RMB2662.00

CD81 抗体 (B-11) AC

sc-166029 AC
500 µg/ml, 25% agarose
RMB3129.00

CD81 抗体 (B-11) HRP

sc-166029 HRP
200 µg/ml
RMB2377.00

CD81 抗体 (B-11) FITC

sc-166029 FITC
200 µg/ml
RMB2482.00

CD81 抗体 (B-11) PE

sc-166029 PE
200 µg/ml
RMB2580.00

CD81 抗体 (B-11) Alexa Fluor® 488

sc-166029 AF488
200 µg/ml
RMB2685.00

CD81 抗体 (B-11) Alexa Fluor® 546

sc-166029 AF546
200 µg/ml
RMB2685.00

CD81 抗体 (B-11) Alexa Fluor® 594

sc-166029 AF594
200 µg/ml
RMB2685.00

CD81 抗体 (B-11) Alexa Fluor® 647

sc-166029 AF647
200 µg/ml
RMB2685.00

CD81 抗体 (B-11) Alexa Fluor® 680

sc-166029 AF680
200 µg/ml
RMB2685.00

CD81 抗体 (B-11) Alexa Fluor® 790

sc-166029 AF790
200 µg/ml
RMB2685.00

Does CD81 antibody (cat #166029) require non-reducing condition or reducing condition for western blot?

Asked by: dustn
Thank you for your question. This antibody recognizes both native and denatured CD81 protein.
Answered by: Tech Support Europe
Date published: 2022-11-30

Hello I would like to know the ratio of fluorochrom per antibody in the Anticorps CD81 (B-11) Alexa Fluor® 647 ; and also the ratio in his isotype ctrl IG g1 anti mouse AF647. Thanks

Asked by: leacurie
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

What westernblot protocol should I use? reducing or non-reducing? I cannot find the information on the sample data sheet. I tried the reducing protocol and get nice single band, but the size is smaller than 20kDa. Thank you

Asked by: yan DK
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Dallas Tech Service
Date published: 2021-04-20

Bonjour , Avez vous la masse molaire de cet Ac?

Asked by: Barbara B
Merci puor votre question. La masse molaire de cet anticorps est 150.000 g/mol.
Answered by: Tech Support Europe
Date published: 2020-11-27

如何领取试用装

Asked by: Anonymous
感谢您的提问。如何获取免费试用装，请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持"。 Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 9
Date published: 2019-03-26

I plan to buy CD81 antibody sc166029 to detect exosome.Can I have some sample for this?

Asked by: Naoya
Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2019-02-02

What is the primary antibody dilution range that we use for CD81?

Asked by: Deepika
Thank you for your question. The dilution range is dependent on the application. Please find the dilution range in the product datasheet for each application, which can be downloaded from this link: https://datasheets.scbt.com/sc-166029.pdf
Answered by: Technical Support
Date published: 2019-01-06

How many FITC molecules are conjugated to each antibody ? and What is the total molecular weight of CD81 Antibody (B-11) FITC (sc-166029 FITC ) please?

Asked by: AgaS
Thank you for your question. The molecular weight of an antibody is ~150 kDa. The ratio is ~4-7 FITC : antibody. The expected molecular weight of CD81 is ~21-26kDa. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-06-28
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Rated 5 out of 5 by from Very good to detect CD81 in exosome extractsWe used CD81 antibody to detect the protein in exosomes isolated from the Bj5ta cell line and worked very well at 1:200 dilution (western blot).
Date published: 2023-07-17
Rated 5 out of 5 by from Very good to detect CD81 in exosome extractsWe used CD81 antibody to detect the protein in exosomes isolated from the Bj5ta cell line and worked very well at 1:200 dilution (western blot).
Date published: 2023-07-17
Rated 5 out of 5 by from Best CD81 antibodysc-166029 (1:500) is by far the best CD81 antibody
Date published: 2023-07-11
Rated 4 out of 5 by from CD81 in exosomes obtained from plasmaWe used this antibody to detect CD81 protein bu WB in extract obtained from exosomes isolated from plasma samples and it worked at 1:200 dilution.
Date published: 2023-05-18
Rated 4 out of 5 by from It detects CD81 on exosome extracts by WBWe used this Ab to identify the presence of CD81 as a marker of exosomes by Western blot using protein extracts from ultracentrifigation pellets and it worked remarkably well
Date published: 2021-11-19
Rated 3 out of 5 by from HTLV-1感染細胞由来エクソソームのCD81をWBで検出5%スキムミルクを使ってブロッキングを行うとバックグラウンドが高く、バンドを検出できませんでした。市販のブロッキング剤を用いるとバンドが検出できました。
Date published: 2021-09-09
Rated 5 out of 5 by from Works very well on MCF7-derived exosomesI tested this antibody on exosomes purified by ultracentrifugation from MCF7 extracellular medium. It produces a single band at the expected size and normal ECL reagent was sufficient to detect it.
Date published: 2021-03-26
Rated 4 out of 5 by from Validated for equine useI have used this antibody for ICC of equine endothelial cells (4ug/ml) and investigating exosomes from equine plasma in an ELISA (1ug/ml). Worked well in both situations.
Date published: 2020-08-13
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CD81 抗体 (B-11) is rated 4.6 out of 5 by 30.
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