Date published: 2025-9-8
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CD81 Antibody (5A6) - Western Blotting - Image 352731
CD81 抗体 (5A6): sc-23962. 对 U-698-M (A), K-562 (B), Jurkat (C) 和 MOLT-4 (D) 全细胞裂解液及人睾丸组织提取物 (E) 中 CD81 表达的 Western 印迹分析.
CD81 抗体 (5A6): sc-23962. 对 U-698-M (A), K-562 (B), Jurkat (C) 和 MOLT-4 (D) 全细胞裂解液及人睾丸组织提取物 (E) 中 CD81 表达的 Western 印迹分析.

CD81 抗体 (5A6): sc-23962

4.1(14)
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说明书
  • CD81抗体(5A6)是小鼠单克隆IgG1 κ, 在127篇文献中引用，规格为200 µg/ml
  • 针对OCI-LY8细胞
  • 抗-CD81 抗体 (5A6) 推荐用于 WB, IP, IF 和 FCM，检测mouse, rat 和human 来源的 CD81
  • 抗CD81抗体（5A6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD81 (5A6): sc-23962的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是CD81 Antibody (5A6) for WB applications. 的首选辅助检测试剂，这些试剂现与CD81 Antibody (5A6) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CD81抗体（5A6）是一种小鼠单克隆IgG1 kappa轻链抗体，专门用于靶向CD81蛋白，也称为TAPA-1，是跨膜蛋白家族的重要成员，具有四个跨膜结构域。CD81广泛表达于造血细胞、神经外胚层细胞和间充质细胞，在细胞生长、信号传导和膜微区组织中发挥重要作用。CD81经历重要的翻译后修饰，包括棕榈酰化和糖基化，这对于其定位、稳定性以及与其他膜蛋白（如CD37、CD53、CD19、CD21和MHC II类抗原）的相互作用至关重要。抗CD81抗体（5A6）是利用OCI-LY8 B淋巴瘤细胞系开发的，可确保通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和流式细胞术（FCM）等方法在小鼠、大鼠和人类物种中有效检测CD81。CD81单克隆抗体（5A6）有多种形式，包括辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物，以及非偶联物，可满足各种实验需求。通过靶向CD81，研究人员可以研究免疫细胞激活、病毒入侵机制以及控制细胞功能的复杂信号通路等关键过程。CD81的翻译后修饰对于理解CD81在癌症转移和免疫失调等病理条件下的作用尤为重要，这使得抗CD81抗体（5A6）成为基础和应用研究的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CD81 抗体 (5A6)

sc-23962
200 µg/ml
RMB2377.00

CD81 (5A6): m-IgG Fc BP-HRP 套装

sc-526643
200 µg Ab; 10 µg BP
RMB2662.00

CD81 (5A6): m-IgG1 BP-HRP 套装

sc-532016
200 µg Ab; 20 µg BP
RMB2662.00

CD81 抗体 (5A6) AC

sc-23962 AC
500 µg/ml, 25% agarose
RMB3129.00

CD81 抗体 (5A6) HRP

sc-23962 HRP
200 µg/ml
RMB2377.00

CD81 抗体 (5A6) FITC

sc-23962 FITC
200 µg/ml
RMB2482.00

CD81 抗体 (5A6) PE

sc-23962 PE
200 µg/ml
RMB2580.00

CD81 抗体 (5A6) Alexa Fluor® 488

sc-23962 AF488
200 µg/ml
RMB2685.00

CD81 抗体 (5A6) Alexa Fluor® 546

sc-23962 AF546
200 µg/ml
RMB2685.00

CD81 抗体 (5A6) Alexa Fluor® 594

sc-23962 AF594
200 µg/ml
RMB2685.00

CD81 抗体 (5A6) Alexa Fluor® 647

sc-23962 AF647
200 µg/ml
RMB2685.00

CD81 抗体 (5A6) Alexa Fluor® 680

sc-23962 AF680
200 µg/ml
RMB2685.00

CD81 抗体 (5A6) Alexa Fluor® 790

sc-23962 AF790
200 µg/ml
RMB2685.00

What is the isoelectric point for CD-81 antibody?

Asked by: Sumita
Thank you for your question. We do not characterize the isoelectric point of our antibodies.
Answered by: BlakeJ
Date published: 2025-07-23

Hello, I have used the CD81 antibody for western blot at 1:1500 in 5% MILK on exosomes from human plasma. Do you know why I get two bands, one at 23 kDa and another one at 48 kDa? Many thanks Juliana

Asked by: Juliana Redondo
Thank you for your question. The expected molecular weight for CD81 is ~ 22-26 kDa. We have not tested this antibody specifically in human plasma, and have not observed a higher molecular weight signal at 48 kDa in the cell lines tested. CD81 has been reported to dimerize (PMID: 20375010) and that may be a consideration. We would also recommend including a secondary antibody control, where you omit the primary antibody from the incubation, to confirm if the higher molecular weight band might be due to non-specific binding of the secondary antibody alone.
Answered by: Technical Support
Date published: 2017-10-16

Hi, I have used this antibody at 1:100 concentration on a western blot (10% SDS-PAGE gel) loading some exosomal protein. Could you explain please why I get not only the 26 kDa band, but also a band at about 50 kDa? Thank you.

Asked by: VivSal
Thank you for your question. We have not tested this antibody on exosomal protein, or observed a 50 kDa band in Western blot. We would recommend including a secondary antibody control (omit primary antibody) to identify if the 50 kDa band may be a result of non-specific binding of the secondary antibody alone. It could also be your secondary antibody binding to heavy chain IgG in your samples. Below is a link to our recommended protocol for Western blotting. Please contact our Technical Service Department if for additional support with troubleshooting the antibody. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-10-16

I have tried using this antibody on a western blot (after running an SDS-PAGE gel) with a homemade K562 lysate as control and did not get a signal at 1:100 dilution. I tried it 3 times previously on other cell lines at 1:200 w/no success. Any suggestions?

Asked by: Tali
Thank you for your question. If you have no signal, we would recommend verifying that your anti-mouse secondary is working well. We would also recommend including a loading control antibody (e.g., Actin or GAPDH) to ensure that your transfer conditions are good, as well as your signal detection conditions (e.g., chemiluminescence or other). We have successfully used this antibody for WB at a dilution of 1:200, loading 50 µg of K-562 whole cell lysate. A link to our complete recommended protocol is provided below. If you continue to have negative results, please contact our Technical Service Department so we can provide further troubleshooting support. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase. If we are unable to successfully troubleshoot your application we will send you a replacement antibody free of charge, or issue a credit or refund if that is not possible https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting .
Answered by: Technical Support
Date published: 2017-04-26

What concentration would you recommend using SC-20048 at, to detect a protein in paraffin-embedded human tissue? We have used 1:1000 successfully for western blots. Please can you reply at your earliest convenience.

Asked by: Deborah Goberdhan
Thank you for your question! We recommend a starting dilution of 1:50 for IHC on paraffin embedded tissue sections. This is a starting dilution, so it is possible that you can use it much more diluted! We also recommend performing an antigen retrieval step, ideally sodium citrate with heat. You can find our recommended protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-02-22

1, What concentration do you recommend using SC-23962 on paraffin embedded human tissue sections? We have this antibody, so would like to try. ; 2. Do you think that SC-23962 FITC would work better and at what conc. for same application, ie IHC(P)?

Asked by: Deborah Goberdhan
Thank you for your question! We recommend a starting dilution of 1:50 for IHC on paraffin embedded tissue sections. We do recommend performing an antigen retrieval step. You can find our recommended protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining You can use the FITC conjugated form, although we do not generally use FITC conjugated antibodies for IHC in house, so I do not have test results for this. If you wish to try it, I would stick with the 1:50 starting dilution as well. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-02-22

Can I use this antibody for staining of mouse tissue, since it is a mouse antibody?

Asked by: Anna99
Yes. CD81 Antibody (5A6): sc-23962 is available conjugated to either phycoerythrin (sc-23962 PE) or fluoroscein (sc-23962 FITC), 200 µg/ml, for IF, IHC(P) and FCM.
Answered by: Tech Service 5
Date published: 2017-01-05
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Rated 4 out of 5 by from Great antibodyI request a sample of the antibody and it worked well!
Date published: 2023-07-31
Rated 4 out of 5 by from buon funzionamentoHo effettuato un solo esperimento per verificare la reattività nel cane e ha funzionato
Date published: 2023-07-14
Rated 1 out of 5 by from WBでバンドが検出できませんでした。エクソソームサンプルでCD81のバンドが全く検出できませんでした。同じサンプルで他のCD81抗体を使うとCD81を検出できたのでサンプルは問題ないと考えています。
Date published: 2021-09-09
Rated 2 out of 5 by from No band in Western blotI cannot see any band in Western blot after a nice transfer (all MW completely transfered; blotting using 1:200 of this antibody and 2nd antibody sc-516102 at 1:1000) of exosomes isolated by ultracentrifugation and further separated by density centrifugation. Perhaps I have low concentration of exosomes, but I expected to get at least a faint band.
Date published: 2021-05-25
Rated 5 out of 5 by from Works well in western blotThe antibody detected CD81 in Mesenchymal stem cells at the expected molecular size.
Date published: 2020-09-18
Rated 5 out of 5 by from Tested on HFFF by FACSThis antibody was tested on HFFF cells by FACS and shown a very strong signal, even at dilution 1:100
Date published: 2018-11-19
Rated 5 out of 5 by from Excellent antibody for ICC stainingThis antibody is gorgeous for ICC staining, please see the attached confocal image. Highly recommended. I tried 1:50 dilution for the first time, and you may further dilute it to 1:100 or even 1:200 to decrease the background a little bit, and it should work even better. I feel the overall quality of SCBT antibodies is getting much better,
Date published: 2018-11-08
Rated 5 out of 5 by from likeMembrane localization observed by Immunofluorescence staining of methanol-fixed embedded HeLa cells, also good for staining.
Date published: 2017-05-07
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CD81 抗体 (5A6) is rated 4.1 out of 5 by 14.
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