Date published: 2025-9-8
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CD47 Antibody (MIAP301) - Western Blotting - Image 361488
CD47 (MIAP301): sc-12731. 小鼠脑组织提取物中 CD47 表达的 Western 印迹分析.
CD47 Antibody (MIAP301) - Western Blotting - Image 361488
CD47 (MIAP301): sc-12731. 小鼠脑组织提取物中 CD47 表达的 Western 印迹分析.
CD47 Antibody (MIAP301) - Flow Cytometry - Image 5872
CD47 (MIAP301) FITC: sc-12731 FITC. 小鼠外周血白细胞的 FCM 分析. 黑线直方图代表同型对照, 正常大鼠 IgG2a: sc-2831.
CD47 Antibody (MIAP301) - Western Blotting - Image 377030
CD47 (MIAP301): sc-12731. 大鼠胸腺组织提取物中 CD47 表达的 Western 印迹分析.
CD47 (MIAP301): sc-12731. 小鼠脑组织提取物中 CD47 表达的 Western 印迹分析.

CD47 抗体 (MIAP301): sc-12731

5.0(7)
寫評論提問

说明书
  • CD47 抗体 MIAP301 是大鼠单克隆 IgG2a, 在22篇文献中引用，提供 200 µg/ml
  • raised against intact CD47 purified from placenta
  • 抗-CD47 抗体 (MIAP301) 推荐用于 WB, IP, IF 和 FCM，检测mouse 和rat 来源的 the immunoglobulin domain of CD47
  • CD47 抗体 (MIAP301) 可偶联 琼脂糖 用于 IP; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD47 (MIAP301): sc-12731的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成CD47 抗体 (MIAP301)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CD47抗体（MIAP301）是一种IgG2a大鼠单克隆CD47抗体，可通过WB、IP、IF和FCM检测来自小鼠和大鼠的CD47免疫球蛋白结构域。CD47抗体（MIAP301）既可以是非偶联的，也可以是多种偶联形式的。CD47是一种细胞膜上的重要蛋白，可调节细胞膜上的离子通量。具体而言，CD47参与细胞粘附到细胞外基质时细胞内钙离子浓度的增加。它还是血小板素（SIRP）C末端细胞结合结构域的受体。Rh-null红细胞中缺乏CD47，但在非红细胞中可参与细胞粘附，可防止红细胞的过早消除。它还可能参与病毒感染后的膜渗透性变化。CD47在造血细胞、上皮细胞、内皮细胞和成纤维细胞上表达，在大脑和间充质细胞中表达强烈。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CD47 抗体 (MIAP301)

sc-12731
200 µg/ml
RMB2377.00

CD47 抗体 (MIAP301) AC

sc-12731 AC
500 µg/ml, 25% agarose
RMB3129.00

CD47 抗体 (MIAP301) FITC

sc-12731 FITC
200 µg/ml
RMB2482.00

CD47 抗体 (MIAP301) PE

sc-12731 PE
200 µg/ml
RMB2580.00

CD47 抗体 (MIAP301) Alexa Fluor® 488

sc-12731 AF488
200 µg/ml
RMB2685.00

CD47 抗体 (MAIP301) Alexa Fluor® 546

sc-12731 AF546
200 µg/ml
RMB2685.00

CD47 抗体 (MIAP301) Alexa Fluor® 594

sc-12731 AF594
200 µg/ml
RMB2685.00

CD47 抗体 (MIAP301) Alexa Fluor® 647

sc-12731 AF647
200 µg/ml
RMB2685.00

CD47 抗体 (MIAP301) Alexa Fluor® 680

sc-12731 AF680
200 µg/ml
RMB2685.00

CD47 抗体 (MIAP301) Alexa Fluor® 790

sc-12731 AF790
200 µg/ml
RMB2685.00

the clone name seems wrong. it is supposed to be MIAP301, not MAIP301.

Asked by: kghimire
Thank you for contacting Santa Cruz Biotechnology. The clone will be updated in 2-3 business days .
Answered by: BlakeJ
Date published: 2023-04-04

先生或女士，您好，请问一下这个CD47抗体 (MAIP301)是否是单克隆阻断抗体，可以用于C57小鼠体内阻断CD47。他的同型匹配抗体那个比较适合。如果买，到货周期多长。

Asked by: count
Thank you for your question. Anti-CD47 Antibody (MAIP301): sc-12731 is designed as a detection antibody for WB, IP, IF and FCM. It has not been tested for any blocking properties and we do not have any internal data that would indicate the possibility of using the antibody for blocking CD47.
Answered by: Tech Support Europe
Date published: 2022-06-23

If I want to use this antibody for Flow Cytometry, what starting dilution do you recommend I use?

Asked by: TinTin
We recommend a starting dilution of 1µg of antibody per million cells. Here is a link to our recommended protocol. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support 9
Date published: 2017-01-18
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Rated 5 out of 5 by from Sryong WBVery nice, clear western blot data; strong signal !!
Date published: 2017-02-01
Rated 5 out of 5 by from Great for Flow CytometryThis antibody is well-cited for a variety of applications and has good data from flow. Strong signal in WB too.
Date published: 2016-12-29
Rated 5 out of 5 by from Publishable data with hamster and mouse transfectedPublishable data with hamster and mouse transfected CHO, Melan-a, and B16F10 - SCBT Publication Review
Date published: 2015-06-04
Rated 5 out of 5 by from Working well in WB with synaptosome fromWorking well in WB with synaptosome from mouse hippocampus
Date published: 2015-04-10
Rated 5 out of 5 by from Produced exceptional FCM data for mouse peripheralProduced exceptional FCM data for mouse peripheral blood leukocytes. -SCBT QC
Date published: 2015-02-16
Rated 5 out of 5 by from This antibody was used in a publication forThis antibody was used in a publication for IP and WB on mice brain tissue - SCBT Publication Review
Date published: 2015-01-05
Rated 5 out of 5 by from Produced exceptional FCM data for mouse peripheralProduced exceptional FCM data for mouse peripheral blood leukocytes. -SCBT QC
Date published: 2013-02-01
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CD47 抗体 (MIAP301) is rated 5.0 out of 5 by 7.
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