Date published: 2026-10-24

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CD47 抗体 (BRIC 126): sc-59079

4.5(6)
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说明书
  • CD47抗体(BRIC 126)是小鼠单克隆IgG2b κ, 在8篇文献中引用,规格为200 µg/ml
  • 针对human来源的红细胞
  • 抗-CD47 抗体 (BRIC 126) 推荐用于 WB, IP, IF 和 FCM,检测human, bovine, porcine 和canine 来源的 CD47
  • 抗CD47抗体(BRIC 126)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP和 2b BP-HRP">m-IgG2b BP-HRP是CD47 Antibody (BRIC 126) 适用于 WB 应用。 的首选辅助检测试剂,这些试剂现与CD47 Antibody (BRIC 126) 打包提供(请参阅下面的订购信息)。
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関連項目

CD47抗体(BRIC 126)是一种小鼠单克隆IgG2b kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和流式细胞术(FCM)检测人、牛、猪和犬源的CD47蛋白。CD47抗体(BRIC 126)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。CD47是一种膜蛋白,在调节细胞膜上的阳离子通量方面起着至关重要的作用,特别是在细胞与细胞外基质粘附时影响细胞内钙浓度。这种功能对于各种细胞过程至关重要,包括迁移、增殖和存活,因为CD47有助于细胞对其环境做出适当反应。此外,CD47还是血栓素C端细胞结合域的受体,而后者对于细胞信号传导和交流非常重要。值得注意的是,Rh-null红细胞不含CD47,但CD47在非红细胞中显著促进细胞粘附,并可能防止红细胞过早被清除。此外,CD47在多种细胞类型中表达,包括造血细胞、上皮细胞、内皮细胞和成纤维细胞,在脑细胞和间充质细胞中的表达尤为强烈,这凸显了CD47在正常生理和潜在病理条件下的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD47 抗体 (BRIC 126) 参考文献:

  1. 神经疾病中的SIRP/CD47信号传导。  |  Zhang, H., et al. 2015. Brain Res. 1623: 74-80. PMID: 25795378
  2. CD47在血液系统恶性肿瘤中的作用。  |  Eladl, E., et al. 2020. J Hematol Oncol. 13: 96. PMID: 32677994
  3. 大脑中的 CD47 与神经变性: 神经炎症通路作用的最新进展。  |  Gheibihayat, SM., et al. 2021. Molecules. 26: PMID: 34203368
  4. TIGIT阻断使AML相关的TIGIT+ M2巨噬细胞重新极化为M1表型,并增加CD47介导的吞噬作用。  |  Brauneck, F., et al. 2022. J Immunother Cancer. 10: PMID: 36549780
  5. CD47-SIRPα轴是癌症免疫疗法的一个很有前景的目标。  |  Hao, Y., et al. 2023. Int Immunopharmacol. 120: 110255. PMID: 37187126
  6. SIRPα控制小鼠和人类CD47依赖性血小板清除。  |  Shoham, M., et al. 2023. bioRxiv.. PMID: 38106070
  7. PBC患者中干扰素-α对CD14+单核细胞上CD47表达的调节。  |  Su, X., et al. 2023. Front Immunol. 14: 1256995. PMID: 38111586
  8. CD47靶向治疗在癌症免疫治疗中的机遇和挑战。  |  Chen, Q., et al. 2023. Oncol Res. 32: 49-60. PMID: 38188674
  9. 基于仿生纳米降解剂的CD47-SIRPα免疫检查点抑制可促进巨噬细胞外泌体对心脏的修复。  |  Gao, J., et al. 2024. Adv Sci (Weinh). 11: e2306388. PMID: 38477522
  10. 解读CD47在癌症免疫治疗中的作用。  |  Liu, Y., et al. 2024. J Adv Res. 63: 129-158. PMID: 39167629

订购信息

产品名称产品编号规格价格数量收藏夹

CD47 抗体 (BRIC 126)

sc-59079
200 µg/ml
CNY2422.00

CD47 (BRIC 126): m-IgG Fc BP-HRP 套装

sc-525676
200 µg Ab; 10 µg BP
CNY2715.00

CD47 (BRIC 126): m-IgG2b BP-HRP 套装

sc-549613
200 µg Ab; 10 µg BP
CNY2715.00

CD47 抗体 (BRIC 126) AC

sc-59079 AC
500 µg/ml, 25% agarose
CNY3189.00

CD47 抗体 (BRIC 126) HRP

sc-59079 HRP
200 µg/ml
CNY2422.00

CD47 抗体 (BRIC 126) FITC

sc-59079 FITC
200 µg/ml
CNY2527.00

CD47 抗体 (BRIC 126) PE

sc-59079 PE
200 µg/ml
CNY2625.00

CD47 抗体 (BRIC 126) Alexa Fluor® 488

sc-59079 AF488
200 µg/ml
CNY2738.00

CD47 抗体 (BRIC 126) Alexa Fluor® 546

sc-59079 AF546
200 µg/ml
CNY2738.00

CD47 抗体 (BRIC 126) Alexa Fluor® 594

sc-59079 AF594
200 µg/ml
CNY2738.00

CD47 抗体 (BRIC 126) Alexa Fluor® 647

sc-59079 AF647
200 µg/ml
CNY2738.00

CD47 抗体 (BRIC 126) Alexa Fluor® 680

sc-59079 AF680
200 µg/ml
CNY2738.00

CD47 抗体 (BRIC 126) Alexa Fluor® 790

sc-59079 AF790
200 µg/ml
CNY2738.00

How was the cross-reactivity with the canine antigen assessed?

Asked by: Vale
Thank you for your question. This antibody was tested on a panel of different canine cells in WB, IP, IF and FCM using our standard recommended protocols for these assays.
Answered by: Tech Support Europe
Date published: 2025-11-12

Do you have a version of this antibody ligated to alkaline phosphatase?

Asked by: Sergio
Thank you for your question. At this time we do not have a version for alkaline phosphatase.
Answered by: BlakeJ
Date published: 2025-01-16

What's the recommende dilution of CD47 (BRIC 126) FITC:antibody for cytometry?

Asked by: SDRC
Thank you for contacting Santa Cruz Biotechnology. We recommend 1ug per million cells. https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: BlakeJ
Date published: 2024-12-26

用于猪上的实验目的条带在30-40kDa并未出现说明说所指的条带位置,并且无法用于免疫荧光,求解答和电话联系

Asked by: bobolink
感谢您的提问。您可以拨打电话:021-60936350,发送邮件到[email protected],或者我们官网上的在线聊天,联系我们的亚洲技术支持团队,了解更多信息。 Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Service SH
Date published: 2024-04-07

Is this a blocking antibody?

Asked by: Pellegrini
Thank you for your question. This antibody is not recommended for neutralization experiments.
Answered by: Santa Cruz TS
Date published: 2021-07-10

What is the recommended positive control for CD47 (BRIC 126): sc-59079?

Asked by: Trav11
Thank you for your question. For CD47 (BRIC 126): sc-59079, we have several recommended positive controls, which include: HeLa Whole Cell Lysate (sc-2200), Jurkat Whole Cell Lysate (sc-2204), HISM Whole Cell Lysate (sc-2229), as well as a few others. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 15
Date published: 2017-01-22
  • y_2026, m_10, d_24, h_5CST
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Rated 5 out of 5 by Music94 from WBWe tested this antibody in WB against red blood cell lysate and EVs, we obtained good results and band at 50 KDa
Date published: 2022-06-21
Rated 5 out of 5 by Bill28 from Gr4at productNice FCM data; strong clear signal; also nice WB data
Date published: 2017-02-02
Rated 5 out of 5 by jerojero from Excellent!This antibody works great in western blot analysis of CD47 expression in human platelet extract.
Date published: 2017-01-11
Rated 4 out of 5 by SS from Positive results in western blot analysisPositive results in western blot analysis of CD47 expression in human platelet extract. -SCBT QC
Date published: 2015-01-12
Rated 4 out of 5 by LR from Robust signal observed in western blot usingRobust signal observed in western blot using human CD47 transfected lysates. -SCBT QC
Date published: 2015-01-12
Rated 4 out of 5 by LM from Produced wonderful FCM data for Jurkat cellsProduced wonderful FCM data for Jurkat cells. -SCBT QC
Date published: 2014-06-08
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CD47 抗体 (BRIC 126) is rated 4.5 out of 5 by 6.
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