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CD34 Antibody (B-6) - Western Blotting - Image 41675
CD34 抗体 (B-6): sc-74499. TF-1 全细胞裂解液中 CD34 表达的 Western 印迹分析.
CD34 抗体 (B-6): sc-74499. TF-1 全细胞裂解液中 CD34 表达的 Western 印迹分析.

CD34 抗体 (B-6): sc-74499

4.7(11)
寫評論提問

说明书
  • CD34抗体(B-6)是小鼠单克隆IgG1 κ, 在68篇文献中引用，规格为200 µg/ml
  • 免疫mouse物种的CD34的氨基酸151-290
  • 抗-CD34 抗体 (B-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 CD34
  • 抗CD34抗体（B-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD34 (B-6): sc-74499的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 CD34 抗体 (B-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD34 抗体 (B-6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CD34抗体（B-6）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类的CD34。CD34（B-6）抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。CD34是一种高度糖基化的跨膜糖蛋白，在识别和分离造血干细胞和祖细胞方面发挥着至关重要的作用，因为CD34出现在它们的表面。CD34表达标志着最原始的干细胞，它们对于血液和免疫系统的发育至关重要。当干细胞分化成谱系祖细胞时，CD34表达逐渐减弱，这使得CD34成为追踪干细胞分化的宝贵标记。CD34出现在小血管内皮细胞以及胎儿和成人神经组织中的某些细胞上，这凸显了CD34在造血和血管生物学中的重要性。CD34细胞质域包含被活化的蛋白激酶C磷酸化的一致性位点，以及丝氨酸、苏氨酸和酪氨酸磷酸化位点，这些位点对于介导影响细胞粘附和迁移的细胞内信号通路至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CD34 抗体 (B-6)

sc-74499
200 µg/ml
RMB2377.00

CD34 (B-6): m-IgG Fc BP-HRP 套装

sc-527031
200 µg Ab; 10 µg BP
RMB2662.00

CD34 (B-6): m-IgG1 BP-HRP 套装

sc-532404
200 µg Ab; 20 µg BP
RMB2662.00

CD34 抗体 (B-6) AC

sc-74499 AC
500 µg/ml, 25% agarose
RMB3129.00

CD34 抗体 (B-6) HRP

sc-74499 HRP
200 µg/ml
RMB2377.00

CD34 抗体 (B-6) FITC

sc-74499 FITC
200 µg/ml
RMB2482.00

CD34 抗体 (B-6) PE

sc-74499 PE
200 µg/ml
RMB2580.00

CD34 抗体 (B-6) Alexa Fluor® 488

sc-74499 AF488
200 µg/ml
RMB2685.00

CD34 抗体 (B-6) Alexa Fluor® 546

sc-74499 AF546
200 µg/ml
RMB2685.00

CD34 抗体 (B-6) Alexa Fluor® 594

sc-74499 AF594
200 µg/ml
RMB2685.00

CD34 抗体 (B-6) Alexa Fluor® 647

sc-74499 AF647
200 µg/ml
RMB2685.00

CD34 抗体 (B-6) Alexa Fluor® 680

sc-74499 AF680
200 µg/ml
RMB2685.00

CD34 抗体 (B-6) Alexa Fluor® 790

sc-74499 AF790
200 µg/ml
RMB2685.00

I wonder what concentration should I use for flow cytometry staining.

Asked by: isaac14
Thank you for your question. We recommend 1 µg normal immunoglobulins per 1 million cells. https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: BlakeJ
Date published: 2023-09-05

Has this antibody been tried with nonhuman primate bone marrow?

Asked by: LCary
Thank you for your question. sc-74499 was tested for CD34 of mouse, rat and human origin
Answered by: Tech Support Europe
Date published: 2021-08-09

Hello! Which class of CD34 does this clone recognize?

Asked by: BHama1992
Thank you for your question. This antibody will detect CD34 of mouse, rat and human origin. It was raised against amino acids 151-290 of CD34 of human origin, the exact epitope has not been mapped.
Answered by: Tech Service
Date published: 2019-05-02

I want to stain my mouse tissue samples. How can I do this using a mouse monoclonal antibody?

Asked by: TinTin
We recommend using a directly conjugated primary antibody. CD34 Antibody (B-6): sc-74499 is available conjugated to agarose (sc-74499 AC), HRP (sc-74499 HRP), phycoerythrin (sc-74499 PE), fluorescein (sc-74499 FITC), Alexa Fluor® 488 (sc-74499 AF488), and Alexa Fluor® 647 (sc-74499 AF647).
Answered by: Technical Service 11
Date published: 2017-01-09
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Rated 4 out of 5 by from good in IHCTried on a mouse kidney, good for immunohistochemical labeling.
Date published: 2022-10-04
Rated 5 out of 5 by from 感觉挺不错的准备购买santa的手套，同个实验室的同学说挺好用的，下手试试看再试试抗体好用不，希望可以得到试用装
Date published: 2019-03-23
Rated 4 out of 5 by from Detection of CD34 by Western blotThis antibody worked well for stem cell and progenitor marker CD34 detection by Western blot.
Date published: 2018-09-25
Rated 5 out of 5 by from Good qualityI used it for IHC staining of paraffin tissue, and it works perfect.
Date published: 2018-04-25
Rated 4 out of 5 by from goodI used it to detect the vessel in A549 cell tumor. It marked the vessels, but also some red blood cells. I'm not sure whether because I put the tumor too long.I look forward to it performance the next time.
Date published: 2017-09-14
Rated 5 out of 5 by from Good qualityGood quality for IF staining of endothelial cells. Specific staining is observed at 1:200 dilution.
Date published: 2017-08-26
Rated 5 out of 5 by from Great choice for CD34Antibody produces strong signal for CD34 detection for mouse, rat, and human samples. Works in multiple applications, including WB, IP, IF, IHC(P), and ELISA. Great choice overall.
Date published: 2017-01-16
Rated 5 out of 5 by from WB: robust band in TFWB: robust band in TF-1 whole cell lysates. -SCBT QC
Date published: 2015-03-15
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CD34 抗体 (B-6) is rated 4.7 out of 5 by 11.
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