Date published: 2025-9-7
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CD34 Antibody (ICO115) - Western Blotting - Image 41666
CD34抗体(ICO115):sc-7324。TF-1全细胞裂解液中CD34表达的Western印迹分析。
CD34抗体(ICO115):sc-7324。TF-1全细胞裂解液中CD34表达的Western印迹分析。

CD34 抗体 (ICO115): sc-7324

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说明书
  • CD34 抗体 ICO115 是小鼠单克隆 IgG1 κ，CD34抗体, 在234篇文献中引用，规格为200 µg/ml
  • 针对慢性髓细胞白血病患者的原代细胞
  • CD34 抗体 (ICO115) 推荐用于 WB, IP, IF 和 FCM，检测mouse, rat 和human 来源的 CD34
  • CD34抗体（ICO115）可与琼脂糖结合用于IP，与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联Alexa Fluor® 405 用于IF, IHC(P) 和FCM
  • 另提供PerCP或Alexa Fluor® 405偶联抗体，规格为 可用于IF、IHC(P)和FCM实验
  • 关于如何获取CD34 (ICO115): sc-7324的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP是CD34 Antibody (ICO115) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与CD34 Antibody (ICO115) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CD34抗体（ICO115）是一种小鼠单克隆IgG1κ轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和流式细胞术（FCM）检测小鼠、大鼠和人类来源的CD34。CD34 (ICO115) 抗体有非结合型和多种结合型，包括琼脂糖、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合型。CD34 是一种高度糖基化的跨膜糖蛋白，在识别和分离造血干细胞和祖细胞方面发挥着关键作用，因为 CD34 主要表达在它们的表面。这种表达尤为重要，因为CD34是大多数原始干细胞的标志物，而原始干细胞对于维持造血和组织再生至关重要。当这些干细胞分化成特定谱系的祖细胞时，CD34的表达逐渐减弱，这凸显了CD34在干细胞生物学和潜在治疗应用中的重要性。CD34存在于小血管内皮细胞以及胎儿和成人神经组织中的某些细胞上，进一步强调了CD34在各种生物学环境中的不同作用。CD34的细胞质结构域包含激活的蛋白激酶C和其他激酶的磷酸化共定位点，这些磷酸化可能影响细胞粘附和迁移的关键信号通路。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD34 抗体 (ICO115) 参考文献:

  1. 癌症患者动员外周血中 CD34 和单核细胞衍生树突状细胞的比较。  |  Syme, R., et al. 2005. Stem Cells. 23: 74-81. PMID: 15625124
  2. CD34 和 CD43 可抑制肥大细胞粘附，是肥大细胞最佳重组所必需的。  |  Drew, E., et al. 2005. Immunity. 22: 43-57. PMID: 15664158
  3. 肿瘤将 CD34+ 细胞从树突状细胞途径分化为内皮细胞。  |  Young, MR. and Cigal, M. 2006. Cancer Immunol Immunother. 55: 558-68. PMID: 16025265
  4. 小鼠 CD34（造血祖细胞和干细胞的标记）的特征。  |  Krause, DS., et al. 1994. Blood. 84: 691-701. PMID: 7519070
  5. CD34+ 阳性造血细胞：生物学和临床应用。  |  Holyoake, TL. and Alcorn, MJ. 1994. Blood Rev. 8: 113-24. PMID: 7524841
  6. CD34 在小鼠胎儿和成年组织的内皮细胞, 造血祖细胞和神经细胞中的表达。  |  Lin, G., et al. 1995. Eur J Immunol. 25: 1508-16. PMID: 7542195
  7. 选择素与碳水化合物的相互作用以及炎症反应的启动。  |  Lasky, LA. 1995. Annu Rev Biochem. 64: 113-39. PMID: 7574477
  8. 细胞粘附受体与哺乳动物早期心脏发育：综述。  |  Buck, CA., et al. 1993. C R Acad Sci III. 316: 838-59. PMID: 8076214
  9. 血小板内皮细胞粘附分子（CD31）。  |  DeLisser, HM., et al. 1993. Curr Top Microbiol Immunol. 184: 37-45. PMID: 8313722

订购信息

产品名称产品编号规格价格数量收藏夹

CD34 抗体 (ICO115)

sc-7324
200 µg/ml
RMB2377.00

CD34 (ICO115): m-IgG1 BP-HRP 套装

sc-531628
200 µg Ab; 20 µg BP
RMB2662.00

CD34 抗体 (ICO115) AC

sc-7324 AC
500 µg/ml, 25% agarose
RMB3129.00

CD34 抗体 (ICO115) FITC

sc-7324 FITC
200 µg/ml
RMB2482.00

CD34 抗体 (ICO115) PE

sc-7324 PE
200 µg/ml
RMB2580.00

CD34 抗体 (ICO115) Alexa Fluor® 488

sc-7324 AF488
200 µg/ml
RMB2685.00

CD34 抗体 (ICO115) Alexa Fluor® 546

sc-7324 AF546
200 µg/ml
RMB2685.00

CD34 抗体 (ICO115) Alexa Fluor® 594

sc-7324 AF594
200 µg/ml
RMB2685.00

CD34 抗体 (ICO115) Alexa Fluor® 647

sc-7324 AF647
200 µg/ml
RMB2685.00

CD34 抗体 (ICO115) Alexa Fluor® 680

sc-7324 AF680
200 µg/ml
RMB2685.00

CD34 抗体 (ICO115) Alexa Fluor® 790

sc-7324 AF790
200 µg/ml
RMB2685.00

CD34 抗体 (ICO115) Alexa Fluor® 405

sc-7324 AF405
200 µg/ml
RMB2685.00

Good morning, I would like to confirm whether the ICO115 clone of CD34 has been published for use in dogs, like the previous C-18 clone.

Asked by: CD34 canine
Thank you for your question. Based on our tests with the antibody, we only recommend CD34 (ICO115): sc-7324 for detection of CD34 of mouse, rat and human origin by WB, IP, IF and FCM. We do not have any internal or customer data on viability in canine cell or tissue samples.
Answered by: Tech Support Europe
Date published: 2025-02-04

I would like to know if this antibody (anti-CD34 sc-7324) was used or tested on dermal vascular tumors in canines. Thank you. 

Asked by: Belen42
Thank you for your question. Anti-CD34 Antibody (ICO115) is only recommended for detection of CD34 of mouse, rat and human origin by WB, IP, IF and FCM. We do not have any data concerning the antibody's viability in canine cell or tissue samples.
Answered by: Tech Support Europe
Date published: 2022-02-07

I would like to have a test sample. Under which email could I contact you?

Asked by: Germán87
Thank you for your question. You can simply click the link above to get in touch with our technical service department.
Answered by: Technical Support Europe
Date published: 2020-10-08

Does this antibodies works with Bovine cells ? we need to isolate mesenchymal cells from bovine adipose tissue.

Asked by: Isma
Thank you for your question. We do not have any data or product citations indicating that this antibody would work with bovine cells.
Answered by: Technical Support
Date published: 2019-01-26

I am looking for a good antibody for hematopoietic cells in dogs, has been used for it?

Asked by: Asela
Thank you for your question. We are not aware of any citations using this CD34 Antibody (ICO115): sc-7324 in samples of canine, or dog origin. Therefore, we do not know if it will react with CD34 in dog. We do offer an alternative antibody that is recommended for detection of CD34 of canine (dog) origin by WB and FCM. That is our mouse monoclonal, CD34 Antibody (1H6): sc-53511. A link to the product information is provided below. This antibody has been cited in 5 publications in samples of dog origin. https://www.scbt.com/scbt/product/cd34-antibody-1h6
Answered by: Technical Support
Date published: 2017-05-17

Someone has tested the antibody on live cell culture without fixation? I have embryoid body cultures to stain and I'm looking for the right antibody which would penetrate deep in the bodies and not require fixation/permeabilization. Thanks

Asked by: SarahIPS
Thank you for your question. We have not tested this CD34 Antibody (ICO115): sc-7324 on live cell culture without fixation or permeabilization. This antibody is very well cited in the primary literature, with many citations using the antibody for immunofluorescent staining or immunhistochemistry. We would recommend consulting with the primary literature to see if anyone else has used this clone for your same intended research application.
Answered by: Technical Support
Date published: 2023-07-25

What negative control should be used when using the conjugated version of CD34 Antibody (ICO115): sc-7324 in flow cytometry?

Asked by: jenni
We recommend using the isotype specific normal mouse IgG conjugated to the same fluorophore. For example, if you are using sc-7324 PE, then you would want to use normal mouse IgG1 PE, sc-2866.
Answered by: Technical Support 2
Date published: 2017-01-09
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Rated 4 out of 5 by from Good signal in WBProvided a good signal in WB (tested in human PBMC samples)
Date published: 2023-10-15
Rated 1 out of 5 by from 蛍光免疫染色4%PFAで固定したラット組織の凍結切片、パラフィン切片を使って蛍光免疫染色を行ってみましたが、特異的なシグナルは検出できませんでした。
Date published: 2018-11-26
Rated 5 out of 5 by from Good antibodyThe antibody works well for IHC with our tested samples.
Date published: 2018-04-25
Rated 4 out of 5 by from gooduse dilution 1: 150 over night for immunofluoresce
Date published: 2018-04-10
Rated 1 out of 5 by from not workAntibody sample, 1:200 dilution for immunostaining of paraffin-embedded sections from a xenograft model of oral cancer, which showing negative staining for endothelial cells.
Date published: 2017-08-18
Rated 5 out of 5 by from Great resultsSuccessfully used in WB applications with rat uterus tissue.
Date published: 2017-08-11
Rated 5 out of 5 by from IF analysisThis is a a directly conjugated primary antibody(sc-7324 FITC ) ,it can easily be used for analysis cd34 expression.the IF protocol is very simple since the secondary antibody no longer needs to be taken in to account.I often use 1:150 for IF analysis use this antibody.
Date published: 2017-07-15
Rated 5 out of 5 by from function goodwe applicated this antibody into the experiment of IHC in hPDLCs and got the negative results which accorded with our predicted results
Date published: 2017-05-31
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CD34 抗体 (ICO115) is rated 4.5 out of 5 by 21.
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