Date published: 2025-9-6

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CD3-ε 抗体 (48-2B): sc-1174

4.5(8)
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说明书
  • CD3-ε Antibody (48-2B) is a Armenian hamster monoclonal IgG1 provided at 200 µg/ml
  • 免疫mouse物种的CD3-ε
  • 推荐用于 mouse 和 rat 来源的CD3-ε IF, IHC(P) 和 FCM检测
  • 还可偶联藻红蛋白 以及 FITC 用于IF, IHC(P) 和 FCM
  • 看 CD3-ε (145-2C11): sc-18871,可查看有标记的 CD3-ε 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前,我们还没有完成CD3-ε 抗体 (48-2B)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

CD3-ε抗体(48-2B)是一种仓鼠单克隆IgG抗体,可通过免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和流式细胞术(FCM)等方法检测小鼠和大鼠样本中的CD3-ε。CD3-ε在T细胞受体(TCR)复合物中起着至关重要的作用,而TCR复合物对于T细胞活化和免疫反应至关重要。CD3-ε位于T细胞表面,是构成CD3复合物的五个不变的多肽链之一,在抗原识别时,CD3复合物对于传递来自TCR的信号至关重要。CD3-ε及其相关链的存在有助于TCR聚集,并随后激活细胞内信号通路,从而促进T细胞增殖和分化。重要的是,CD3-ε包含免疫受体酪氨酸基激活基序(ITAM),该基序对于信号分子(如ZAP-70)的募集至关重要,后者进一步传播激活信号。CD3-ε能够与信号级联中的其他蛋白相互作用,这凸显了它在适应性免疫反应中的重要性,使CD3-ε成为免疫学和治疗应用研究的重要靶标。抗CD3-ε抗体(48-2B)是研究T细胞生物学和免疫反应机制的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

CD3-ε 抗体 (48-2B)

sc-1174
200 µg/ml
RMB2377.00

CD3-ε 抗体 (48-2B) FITC

sc-1174 FITC
200 µg/ml
RMB2482.00

CD3-ε 抗体 (48-2B) L

sc-1174 L
200 µg/0.1 ml
RMB2377.00

CD3-ε 抗体 (48-2B) PE

sc-1174 PE
200 µg/ml
RMB2580.00

Can't make this antibody work on mouse FFPE spleen tissue. Have tried various pre-treatment (EDTA, citrate) but no signal so far using 1/30 dilution and 1hour incubation.Can someone recommand a protocol that works? Thank you.

Asked by: Clement_ipsen
Thank you for your question. Please contact our technical service directly so we can assist with this issue and resolve the problem.
Answered by: Tech Support Europe
Date published: 2019-11-22

Can CD3-ε (48-2B): sc-1174 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD3-ε (48-2B): sc-1174 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from No working on FFPE mouse spleenCan't make this antibody work on FFPE mouse spleen under various pre-treatment.
Date published: 2019-11-21
Rated 5 out of 5 by from Produced excellent FCM data for mouse peripheralProduced excellent FCM data for mouse peripheral blood leukocytes. -SCBT QC
Date published: 2015-06-01
Rated 5 out of 5 by from Superb immunoperoxidase membrane and cytoplasmicSuperb immunoperoxidase membrane and cytoplasmic staining in formalin-fixed, paraffin-embedded normal mouse lymph node. -SCBT QC
Date published: 2015-05-15
Rated 5 out of 5 by from Published IHC(P) data for use in mouse kidneyPublished IHC(P) data for use in mouse kidney (infiltrating T-cells / macrophages). -SCBT Publication Review
Date published: 2015-04-03
Rated 5 out of 5 by from Published data for IF in mouse carotid arteriesPublished data for IF in mouse carotid arteries. -SCBT Publication Review
Date published: 2015-03-26
Rated 5 out of 5 by from Published IHC(P) data for use in rat lung samplePublished IHC(P) data for use in rat lung sample. -SCBT Publication Review
Date published: 2015-03-25
Rated 5 out of 5 by from Published IHC(P) data for use in mouse embryonicPublished IHC(P) data for use in mouse embryonic stem (ES) cells. -SCBT Publication Review
Date published: 2015-01-06
Rated 5 out of 5 by from Nice indirect FCM data of TKNice indirect FCM data of TK-1 cells stained with CD3-e . -SCBT QC
Date published: 2013-12-30
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CD3-ε 抗体 (48-2B) is rated 4.5 out of 5 by 8.
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