Date published: 2026-11-5

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CD3-ε Antibody (48-2B) - Flow Cytometry - Image 143271
CD3-e (48-2B): sc-1174. 用 CD3-e (48-2B) 染色的 TK-1 细胞的间接 FCM 分析, 然后用 FITC 结合的山羊抗亚美尼亚仓鼠 IgG: sc-2446 进行染色. 黑线直方图代表同型对照, 正常亚美尼亚仓鼠: sc-3886.
CD3-e (48-2B): sc-1174. 用 CD3-e (48-2B) 染色的 TK-1 细胞的间接 FCM 分析, 然后用 FITC 结合的山羊抗亚美尼亚仓鼠 IgG: sc-2446 进行染色. 黑线直方图代表同型对照, 正常亚美尼亚仓鼠: sc-3886.

CD3-ε 抗体 (48-2B): sc-1174

4.5(8)
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说明书
  • CD3-ε Antibody (48-2B) is a Armenian hamster monoclonal IgG1 provided at 200 µg/ml
  • 免疫mouse物种的CD3-ε
  • 推荐用于 mouse 和 rat 来源的CD3-ε IF, IHC(P) 和 FCM检测
  • 还可偶联藻红蛋白 以及 FITC 用于IF, IHC(P) 和 FCM
  • 看 CD3-ε (145-2C11): sc-18871，可查看有标记的 CD3-ε 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前，我们还没有完成CD3-ε 抗体 (48-2B)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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研究信息

関連項目

额外CD3抗体，包括CD3-δ, CD3-ε, CD3-γ和CD3-ζ

CD3-ε抗体（48-2B）是一种仓鼠单克隆IgG抗体，可通过免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）等方法检测小鼠和大鼠样本中的CD3-ε。CD3-ε在T细胞受体（TCR）复合物中起着至关重要的作用，而TCR复合物对于T细胞活化和免疫反应至关重要。CD3-ε位于T细胞表面，是构成CD3复合物的五个不变的多肽链之一，在抗原识别时，CD3复合物对于传递来自TCR的信号至关重要。CD3-ε及其相关链的存在有助于TCR聚集，并随后激活细胞内信号通路，从而促进T细胞增殖和分化。重要的是，CD3-ε包含免疫受体酪氨酸基激活基序（ITAM），该基序对于信号分子（如ZAP-70）的募集至关重要，后者进一步传播激活信号。CD3-ε能够与信号级联中的其他蛋白相互作用，这凸显了它在适应性免疫反应中的重要性，使CD3-ε成为免疫学和治疗应用研究的重要靶标。抗CD3-ε抗体（48-2B）是研究T细胞生物学和免疫反应机制的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD3-ε 抗体 (48-2B) 参考文献:

  1. CD3 epsilon介导的信号在T细胞发育中的作用。  |  Sommers, CL., et al. 2000. J Exp Med. 192: 913-19. PMID: 10993922
  2. 在T细胞中，N端和C端CD3-epsilon-ITAM酪氨酸的磷酸化受到不同调节。  |  Guirado, M., et al. 2002. Biochem Biophys Res Commun. 291: 574-81. PMID: 11855827
  3. CD3 epsilon招募Nck揭示了配体诱导的构象变化，这对于T细胞受体信号传导和突触形成至关重要。  |  Gil, D., et al. 2002. Cell. 109: 901-12. PMID: 12110186
  4. Nck-CD3 epsilon相互作用与体内T细胞活化的相关性。  |  Borroto, A., et al. 2014. J Immunol. 192: 2042-53. PMID: 24470497
  5. 在NOD小鼠和CD3 epsilon链转基因临床前小鼠模型中，粘膜注射CD3特异性单克隆抗体可抑制糖尿病。  |  Kuhn, C., et al. 2017. J Autoimmun. 76: 115-122. PMID: 27745778
  6. 小鼠CD3-epsilon基因的表征和表达。  |  Clevers, H., et al. 1988. Proc Natl Acad Sci U S A. 85: 8623-7. PMID: 2973066
  7. 锦鲤肾脏中CD3-Epsilon的特异性表达。  |  Miyazawa, R., et al. 2018. Front Immunol. 9: 1321. PMID: 29951063
  8. CD3 epsilon和CD3 zeta细胞质结构域可独立产生T细胞发育和功能的信号。  |  Shinkai, Y., et al. 1995. Immunity. 2: 401-11. PMID: 7719942
  9. CD3 epsilon转基因的过度表达会阻碍T淋巴细胞发育。  |  Wang, B., et al. 1995. Int Immunol. 7: 435-48. PMID: 7794823
  10. 原祖细胞中CD3-epsilon的过度表达可作为癌基因。  |  Wang, B., et al. 1997. Mol Med. 3: 72-81. PMID: 9132282

订购信息

产品名称产品编号规格价格数量收藏夹

CD3-ε 抗体 (48-2B)

sc-1174
200 µg/ml
CNY2422.00

CD3-ε 抗体 (48-2B) FITC

sc-1174 FITC
200 µg/ml
CNY2527.00

CD3-ε 抗体 (48-2B) L

sc-1174 L
200 µg/0.1 ml
CNY2422.00

CD3-ε 抗体 (48-2B) PE

sc-1174 PE
200 µg/ml
CNY2625.00

Can't make this antibody work on mouse FFPE spleen tissue. Have tried various pre-treatment (EDTA, citrate) but no signal so far using 1/30 dilution and 1hour incubation.Can someone recommand a protocol that works? Thank you.

Asked by: Clement_ipsen
Thank you for your question. Please contact our technical service directly so we can assist with this issue and resolve the problem.
Answered by: Tech Support Europe
Date published: 2019-11-22

Can CD3-ε (48-2B): sc-1174 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD3-ε (48-2B): sc-1174 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by Clement_ipsen from No working on FFPE mouse spleenCan't make this antibody work on FFPE mouse spleen under various pre-treatment.
Date published: 2019-11-21
Rated 5 out of 5 by SS from Produced excellent FCM data for mouse peripheralProduced excellent FCM data for mouse peripheral blood leukocytes. -SCBT QC
Date published: 2015-06-01
Rated 5 out of 5 by NZ from Superb immunoperoxidase membrane and cytoplasmicSuperb immunoperoxidase membrane and cytoplasmic staining in formalin-fixed, paraffin-embedded normal mouse lymph node. -SCBT QC
Date published: 2015-05-15
Rated 5 out of 5 by CJ from Published IHC(P) data for use in mouse kidneyPublished IHC(P) data for use in mouse kidney (infiltrating T-cells / macrophages). -SCBT Publication Review
Date published: 2015-04-03
Rated 5 out of 5 by TS from Published data for IF in mouse carotid arteriesPublished data for IF in mouse carotid arteries. -SCBT Publication Review
Date published: 2015-03-26
Rated 5 out of 5 by HF from Published IHC(P) data for use in rat lung samplePublished IHC(P) data for use in rat lung sample. -SCBT Publication Review
Date published: 2015-03-25
Rated 5 out of 5 by TO from Published IHC(P) data for use in mouse embryonicPublished IHC(P) data for use in mouse embryonic stem (ES) cells. -SCBT Publication Review
Date published: 2015-01-06
Rated 5 out of 5 by TO from Nice indirect FCM data of TKNice indirect FCM data of TK-1 cells stained with CD3-e . -SCBT QC
Date published: 2013-12-30
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CD3-ε抗体 (48-2B) is rated 4.5 out of 5 by 8.
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