Date published: 2025-9-6
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CD3-ε Antibody (UCH-T1) - Western Blotting - Image 354153
CD3-ε 抗体 (UCH-T1): sc-1179. Jurkat (A), SUP-T1 (B) 和 CCRF-HSB-2 (C) 全细胞裂解液中 CD3-ε 表达的 Western 印迹分析.
CD3-ε 抗体 (UCH-T1): sc-1179. Jurkat (A), SUP-T1 (B) 和 CCRF-HSB-2 (C) 全细胞裂解液中 CD3-ε 表达的 Western 印迹分析.

CD3-ε 抗体 (UCH-T1): sc-1179

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说明书
  • CD3-epsilon 抗体 UCH-T1 是小鼠单克隆 IgG1 κ，CD3-ε抗体, 在31篇文献中引用，规格为200 µg/ml
  • 针对 human 婴儿胸腺细胞和塞萨里综合征患者淋巴细胞
  • CD3-epsilon 抗体 (UCH-T1) 推荐用于WB, IP, IF, IHC(P) 和 FCM，检测human 来源的 CD3-ε
  • 抗CD3-epsilon抗体（UCH-T1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联Alexa Fluor® 405 用于IF, IHC(P) 和FCM
  • 关于如何获取CD3-ε (UCH-T1): sc-1179的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 CD3-ε 抗体 (UCH-T1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD3-ε 抗体 (UCH-T1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 CD3 抗体 包括 CD3-δ, CD3-ε, CD3-γ和CD3-ζ

CD3-ε抗体（UCH-T1）是一种小鼠单克隆IgG1κ轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）检测人类CD3-epsilon蛋白。抗CD3-ε抗体（UCH-T1）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。CD3-epsilon蛋白作为T细胞受体（TCR）复合物的一个组成部分，在免疫反应中发挥着至关重要的作用，而T细胞受体复合物对于T细胞的活化和信号传导至关重要。CD3-epsilon位于T细胞表面，是一个多亚基复合物的一部分，该复合物在识别外来抗原时传递信号，从而激活和增殖T细胞。这一过程对于适应性免疫反应至关重要，使人体能够有效应对病原体。CD3复合物由多个不变的多肽链组成，包括CD3-γ、CD3-δ和CD3-ζ，它们共同促进TCR结合引发的信号级联。值得注意的是，这些链中免疫受体酪氨酸基激活基序（ITAM）的存在对于招募信号分子（如ZAP-70）至关重要，后者进一步传播激活信号。了解CD3-epsilon的功能和重要性对于制定针对包括癌症和自身免疫性疾病在内的各种疾病中T细胞反应的治疗策略至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD3-ε 抗体 (UCH-T1) 参考文献:

  1. TCR的CD3ε亚基包含内吞信号。  |  Borroto, A., et al. 1999. J Immunol. 163: 25-31. PMID: 10384095
  2. T 细胞抗原受体的结构, 组装和细胞内转运。  |  Exley, M., et al. 1991. Semin Immunol. 3: 283-97. PMID: 1686832
  3. T 细胞抗原受体的信号转导。  |  Weiss, A., et al. 1991. Semin Immunol. 3: 313-24. PMID: 1839225
  4. 体外制备和表征人类CD3 epsilon epsilon同源二聚体和CD3 epsilon gamma和CD3 epsilon delta异源二聚体。  |  Su, Z., et al. 2009. Int J Mol Med. 24: 437-44. PMID: 19724882
  5. 信号转导。Zapping tandem SH2 domains.  |  Weiss, A. 1995. Nature. 377: 17-8. PMID: 7659151
  6. 蛋白酪氨酸激酶 ZAP-70 与 T 细胞受体相互作用的分子基础。  |  Hatada, MH., et al. 1995. Nature. 377: 32-8. PMID: 7659156
  7. ZAP-70 与磷酸化的 T 细胞受体 zeta 和 eta 结合可增强其自身磷酸化，并为含 SH2 结构域的蛋白质生成特异性结合位点。  |  Neumeister, EN., et al. 1995. Mol Cell Biol. 15: 3171-8. PMID: 7760813
  8. CD3 zeta 家族二聚体的不同细胞质结构可调节 T 细胞的激活信号和功能。  |  Aoe, T., et al. 1994. Int Immunol. 6: 1671-9. PMID: 7865460
  9. 蛋白酪氨酸激酶和蛋白酪氨酸磷酸酶在 T 细胞抗原受体信号转导中的作用。  |  Chan, AC., et al. 1994. Annu Rev Immunol. 12: 555-92. PMID: 8011291
  10. 靶向干扰 CD3 eta 基因座导致小鼠高致死率：Oct-1 转录在反向链上的调节。  |  Ohno, H., et al. 1994. EMBO J. 13: 1157-65. PMID: 8131747
  11. TCR/CD3复合物的组装：CD3 epsilon/delta和CD3 epsilon/gamma二聚体与TCR alpha和TCR beta链结合不明确。双TCR异二聚体模型的证据。  |  San José, E., et al. 1998. Eur J Immunol. 28: 12-21. PMID: 9485181
  12. FOXM1参与滋养层细胞迁移和早期滋养层细胞侵袭：在胚泡着床中的潜在作用。  |  Wang, N., et al. 1998. Int Immunol. 10: 1777-88. PMID: 9885898

订购信息

产品名称产品编号规格价格数量收藏夹

CD3-ε 抗体 (UCH-T1)

sc-1179
200 µg/ml
RMB2377.00

CD3-ε (UCH-T1): m-IgG Fc BP-HRP 套装

sc-526465
200 µg Ab; 10 µg BP
RMB2662.00

CD3-ε (UCH-T1): m-IgG1 BP-HRP 套装

sc-531838
200 µg Ab; 20 µg BP
RMB2662.00

CD3-ε 抗体 (UCH-T1) AC

sc-1179 AC
500 µg/ml, 25% agarose
RMB3129.00

CD3-ε 抗体 (UCH-T1) HRP

sc-1179 HRP
200 µg/ml
RMB2377.00

CD3-ε 抗体 (UCH-T1) FITC

sc-1179 FITC
200 µg/ml
RMB2482.00

CD3-ε 抗体 (UCH-T1) PE

sc-1179 PE
200 µg/ml
RMB2580.00

CD3-ε 抗体 (UCH-T1) Alexa Fluor® 488

sc-1179 AF488
200 µg/ml
RMB2685.00

CD3-ε 抗体 (UCH-T1) Alexa Fluor® 546

sc-1179 AF546
200 µg/ml
RMB2685.00

CD3-ε 抗体 (UCH-T1) Alexa Fluor® 594

sc-1179 AF594
200 µg/ml
RMB2685.00

CD3-ε 抗体 (UCH-T1) Alexa Fluor® 647

sc-1179 AF647
200 µg/ml
RMB2685.00

CD3-ε 抗体 (UCH-T1) Alexa Fluor® 680

sc-1179 AF680
200 µg/ml
RMB2685.00

CD3-ε 抗体 (UCH-T1) Alexa Fluor® 790

sc-1179 AF790
200 µg/ml
RMB2685.00

CD3-ε 抗体 (UCH-T1) Alexa Fluor® 405

sc-1179 AF405
200 µg/ml
RMB2685.00

I have used your CD3e (sc-1127) for years on various animal tissue. Now I see that it has been discontinued and the recommended replacement is human specific. Can I get a sample of a CD3 antibody that you think might have the best chance of working in dog

Asked by: MMWi
Thank you for your question. We are currently offering a free 10 µg sample of our mouse monoclonal antibody, CD3-ε (UCH-T1): sc-1179, and I would be happy to send it to you to test in your samples. Unfortunately, we do not have any evidence of any of our CD3-ε antibodies working for dog at this time. And we do not provide sample sizes for any of the other CD3-ε antibodies. Please contact our Technical Service Department if you would like help with performing sequence analysis to predict if any of our other antibodies might be expected to cross react with dog. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-07-05

Can CD3-ε (UCH-T1): sc-1179 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD3-ε (UCH-T1): sc-1179 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from it's not suited to IHC-PI tried IHC-P with this antibody, but there is no signal.
Date published: 2016-12-04
Rated 4 out of 5 by from Great FCM data of Jurkat cellsGreat FCM data of Jurkat cells. -SCBT QC
Date published: 2015-06-01
Rated 4 out of 5 by from Strong band in WB in Jurkat whole cell lysateStrong band in WB in Jurkat whole cell lysate. -SCBT QC
Date published: 2015-05-05
Rated 4 out of 5 by from Produced wonderful FCM data for human peripheralProduced wonderful FCM data for human peripheral blood leukocytes. -SCBT QC
Date published: 2015-03-15
Rated 4 out of 5 by from Published IP and WB data for use in mouse WEHIPublished IP and WB data for use in mouse WEHI-231 cell line. -SCBT Publication Review
Date published: 2015-01-24
Rated 4 out of 5 by from Robust immunoperoxidase membrane stainingRobust immunoperoxidase membrane staining in formalin-fixed, paraffin-embedded normal human tonsil. -SCBT QC
Date published: 2015-01-07
Rated 4 out of 5 by from Published data for IP in human Jurkat cellsPublished data for IP in human Jurkat cells. -SCBT Publication Review
Date published: 2015-01-07
Rated 4 out of 5 by from Produced wonderful indirect FCM data forProduced wonderful indirect FCM data for HuT 78 cells stained with CD3-e . -SCBT QC
Date published: 2014-05-06
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CD3-ε 抗体 (UCH-T1) is rated 3.9 out of 5 by 8.
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