Date published: 2025-9-7

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CD163 抗体 (ED2): sc-58965

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说明书
  • CD163抗体(ED2)是小鼠单克隆IgG1 κ, 在57篇文献中引用,规格为100 µg/ml
  • 与rat来源的脾细胞匀浆进行比对
  • 推荐用于 mouse 和 rat 来源的the CD163 cell surface glycoprotein WB, IP, IF, IHC(P) 和 FCM检测
  • 目前,我们还没有完成CD163 抗体 (ED2)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

CD163 抗体(ED2)是一种小鼠单克隆 IgG1 kappa 轻链抗体,通过免疫印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和流式细胞术(FCM)等方法证明,该抗体能在小鼠和大鼠体内准确靶向 CD163。CD163 又称 M130 或 SCARI1,是一种重要的巨噬细胞相关抗原,属于富含半胱氨酸的清道夫受体(SCR)超家族,其特征是具有多个 SRCR 结构域,可促进与血红蛋白-血红蛋白复合物的结合。CD163 的结构配置具有一系列胞外 SRCR 结构域、一个跨膜区和一个胞质尾部,对于介导血红蛋白-aptoglobin 复合物的内吞和清除至关重要,在抗炎反应和铁代谢中发挥着重要作用。这种精确的结构排列使 CD163 能以钙依赖方式有效识别并高亲和力地结合配体,确保有效清除游离血红蛋白,防止溶血过程中的氧化应激。此外,CD163 的各种异构体在胞质结构域和潜在的磷酸化位点上存在差异,这表明它们具有不同的信号机制,可调节巨噬细胞的功能和对炎症刺激的反应。CD163 定位于巨噬细胞膜上,其次是单核细胞膜上,这对于与细胞外配体相互作用、促进细胞平衡和免疫调节至关重要。抗 CD163 抗体 (ED2) 有非共轭型和多种共轭型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和各种 Alexa Fluor® 共轭物,为各种研究应用提供了多种选择。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

CD163 抗体 (ED2)

sc-58965
100 µg/ml
RMB2504.00

CD163 Antibody (ED2): sc-58965染细胞膜是否需要用triton透化

Asked by: lingyu
感谢您的提问。CD163 Antibody (ED2): sc-58965染细胞膜不需要用triton透化。 Thank you for your question. There is no need to proceed permeabilization treatment when using CD163 Antibody (ED2): sc-58965 to stain cytomembrane.
Answered by: Technical Service SH
Date published: 2022-08-25

cd163 (sc-58965) is not explicitly stated to provide a 10ug sample. Can I apply for a sample of this product as well?

Asked by: minyoung
Thank you for your question. This antibody is currently not available in sample format.
Answered by: Tech Support Europe
Date published: 2021-03-03

What protocol do you recommend for antigen retrieval for this antibody for FFPE IHC-P?

Asked by: livlab
Thank you for your question. You can find our recommended method for antigen retrieval in our standard protocol, which is available under this link: https://www.scbt.com/resources/protocols/immunoperoxidase-staining.
Answered by: Technical Support Europe
Date published: 2020-10-21

Is CD163 Antibody (ED2) sc-58965 appropriate for use in FACS Analysis of mouse tissue?

Asked by: Anonymous
Thank you for your question. This antibody has been validated for flow cytometry (FCM) in rat tissues. The antibody is currently recommended and warrantied for this application in mouse and rat tissues. We are still generating data to show on our website. Please check back for updates!
Answered by: Technical Support
Date published: 2020-05-04

Is CD163 Antibody (ED2): sc-58965 appropriate for use in IHC with paraffin-embedded sections?

Asked by: jenni
Thank you for your question. While this antibody has not been validated for use with paraffin-embedded samples by our labs, there are publications citing this antibody for use in IHC(P). Please contact our Technical Service Department for further details and recommendations.
Answered by: Technical Support 15
Date published: 2017-01-09

Is there a positive control recommended for CD163 Antibody (ED2): sc-58965? Thanks.

Asked by: Dyana
Thank you for your question. We have not characterized a recommended positive control for this CD163 antibody, sc-58965. Please contact Technical Service if you require any further assistance at 800-457-3801 or [email protected]
Answered by: Technical Service
Date published: 2016-11-01
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Rated 5 out of 5 by from good antibodyMouse cell tissue CD163 WB dilution 1:500 appeared strong signal.
Date published: 2017-05-07
Rated 5 out of 5 by from Achieve the expected molecular weightCD163 antibody provides clear and specific results, got strong bands for WB
Date published: 2017-04-24
Rated 5 out of 5 by from good antibodyWell-cited antibody that works well to detect the rat protein in many applications.
Date published: 2017-01-12
Rated 5 out of 5 by from Successfully used in IHC with rat boneSuccessfully used in IHC with rat bone - SCBT Publication Review
Date published: 2015-02-21
Rated 5 out of 5 by from Publishable WB data with rat ED1Publishable WB data with rat ED1-stained cells - SCBT Publication Review
Date published: 2015-02-08
Rated 5 out of 5 by from IHCP publishable data on rat bone tissueIHCP publishable data on rat bone tissue - SCBT Publication Review
Date published: 2015-01-20
Rated 5 out of 5 by from This antibody was used for IHC with rat spinalThis antibody was used for IHC with rat spinal cord - SCBT Publication Review
Date published: 2014-01-17
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CD163 抗体 (ED2) is rated 5.0 out of 5 by 7.
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