Date published: 2025-9-6

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CD163 抗体 (GHI/61): sc-20066

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说明书
  • CD163 抗体 GHI/61 是小鼠单克隆 IgG1 κ,CD163抗体, 在48篇文献中引用,规格为200 µg/ml
  • 针对毛细胞白血病
  • CD163 抗体 (GHI/61) 推荐用于WB, IP, IF, IHC(P) 和 FCM,检测human 来源的 CD163
  • 抗CD163抗体(GHI/61)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD163 (GHI/61): sc-20066免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 CD163 抗体 (GHI/61) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD163 抗体 (GHI/61) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

CD163抗体(GHI/61)是一种小鼠单克隆IgG1 kappa轻链抗体,靶向人类CD163蛋白,因此抗CD163抗体(GHI/61)成为研究巨噬细胞生物学和相关途径的研究人员的重要工具。CD163,也称为M130或SCARI1,是富含半胱氨酸的清除受体(SRCR)超家族的一员,是巨噬细胞的重要标志物,在较小程度上也是单核细胞的标志物。CD163在清除血红蛋白-结合珠蛋白复合物方面发挥着重要作用,通过钙依赖性高亲和力相互作用介导其内吞作用,确保有效清除游离血红蛋白,从而防止氧化应激和炎症。CD163受体不仅参与血红蛋白清除,还参与抗炎反应,使CD163成为免疫调节的重要参与者,以及各种炎症疾病的潜在治疗靶点。CD163单克隆抗体(GHI/61)推荐用于多种应用,包括蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和流式细胞术(FCM),确保在各种实验设置中的通用性。抗CD163抗体(GHI/61)有非结合型和一系列结合型,如琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和各种Alexa Fluor®染料,有助于在不同检测条件下精确检测和可视化CD163。多种CD163同种型在细胞质域和磷酸化位点方面存在差异,表明存在不同的信号传导机制,这进一步强调了准确检测和研究CD163的重要性。CD163由位于人类12p13.31号染色体上的基因编码,其表达和调节对于维持免疫稳态至关重要,而了解CD163的作用有助于深入了解健康和疾病中巨噬细胞介导的进程。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD163 抗体 (GHI/61) 参考文献:

  1. 人类 CD163 (M130) 基因的基因组结构和染色体定位:清道夫受体富半胱氨酸超家族的成员。  |  Ritter, M., et al. 1999. Biochem Biophys Res Commun. 260: 466-74. PMID: 10403791
  2. 人类巨噬细胞上 CD 163 的调控:CD163 的交联诱导信号传递和活化。  |  Van den Heuvel, MM., et al. 1999. J Leukoc Biol. 66: 858-66. PMID: 10577520
  3. 促炎和抗炎刺激对人类单核细胞和巨噬细胞中清道夫受体 CD163 表达的调控。  |  Buechler, C., et al. 2000. J Leukoc Biol. 67: 97-103. PMID: 10648003
  4. 人类单核细胞表达 CD163,它由 IL-10 上调,与 p155 相同。  |  Sulahian, TH., et al. 2000. Cytokine. 12: 1312-21. PMID: 10975989
  5. 通过原位杂交和体细胞杂交分析,将编码新型清道夫受体 M160 的基因 CD163B 定位于人类染色体 12p13.3。  |  Stover, CM., et al. 2000. Cytogenet Cell Genet. 90: 246-7. PMID: 11124526
  6. 鉴定血红蛋白清道夫受体。  |  Kristiansen, M., et al. 2001. Nature. 409: 198-201. PMID: 11196644
  7. CD163 与酪蛋白激酶 II(CKII)调节亚基的相互作用以及 CD163 信号传导对 CKII 和蛋白激酶 C 的依赖性。  |  Ritter, M., et al. 2001. Eur J Immunol. 31: 999-1009. PMID: 11298324
  8. 一种新的巨噬细胞分化抗原,属于清道夫受体超家族。  |  Law, SK., et al. 1993. Eur J Immunol. 23: 2320-5. PMID: 8370408

订购信息

产品名称产品编号规格价格数量收藏夹

CD163 抗体 (GHI/61)

sc-20066
200 µg/ml
RMB2377.00

CD163 (GHI/61): m-IgG Fc BP-HRP 套装

sc-526597
200 µg Ab; 10 µg BP
RMB2662.00

CD163 (GHI/61): m-IgG1 BP-HRP 套装

sc-531970
200 µg Ab; 20 µg BP
RMB2662.00

CD163 抗体 (GHI/61) AC

sc-20066 AC
500 µg/ml, 25% agarose
RMB3129.00

CD163 抗体 (GHI/61) HRP

sc-20066 HRP
200 µg/ml
RMB2377.00

CD163 抗体 (GHI/61) FITC

sc-20066 FITC
200 µg/ml
RMB2482.00

CD163 抗体 (GHI/61) PE

sc-20066 PE
200 µg/ml
RMB2580.00

CD163 抗体 (GHI/61) Alexa Fluor® 488

sc-20066 AF488
200 µg/ml
RMB2685.00

CD163 抗体 (GHI/61) Alexa Fluor® 546

sc-20066 AF546
200 µg/ml
RMB2685.00

CD163 抗体 (GHI/61) Alexa Fluor® 594

sc-20066 AF594
200 µg/ml
RMB2685.00

CD163 抗体 (GHI/61) Alexa Fluor® 647

sc-20066 AF647
200 µg/ml
RMB2685.00

CD163 抗体 (GHI/61) Alexa Fluor® 680

sc-20066 AF680
200 µg/ml
RMB2685.00

CD163 抗体 (GHI/61) Alexa Fluor® 790

sc-20066 AF790
200 µg/ml
RMB2685.00

Does it react with mice sample

Asked by: XJ Lin
感谢您的提问。 我们还没有使用这支抗体在小鼠样本中验证过。 如果您有更多问题,欢迎通过,电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your question. We have not yet tested sc-20066, Anti-CD163 Antibody (GHI/61) on samples from mouse. If you have any further questions, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Ling Chen
Date published: 2022-09-02

Against which amino acid sequence is this antibody raised? 

Asked by: Shreya
Thank you for your question. This CD163 antibody was raised against hairy cell leukaemia. The exact epitope has not been mapped.
Answered by: Tech Service
Date published: 2019-10-08

Which negative control should be used when using the FITC conjugated CD163 (GHI/61): sc-20066 in flow cytometry?

Asked by: jenni
Thank you for your inquiry. We recommend using normal mouse IgG conjugated to FITC, sc-2339 as a negative control
Answered by: Technical Support 3
Date published: 2017-01-09
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Rated 4 out of 5 by from Good antibody for western blotI used this antibody in western blot on human cell. Good specificity of the band and no background.
Date published: 2021-11-23
Rated 4 out of 5 by from good antibody for IFI used this antibody in IF in frozen human colon tissue sections and it works very well.
Date published: 2018-06-20
Rated 4 out of 5 by from welli see, this antibody works well in WB experiment, but not very well in IF
Date published: 2018-01-08
Rated 4 out of 5 by from GOODNice product for HepG2; needs further testing for WB and IHC
Date published: 2017-12-02
Rated 5 out of 5 by from EXCELENTCD168, in the course of the experiment, the 1:100 titer of immunohistochemistry was in line with my experimental requirements
Date published: 2017-09-21
Rated 4 out of 5 by from goodWestern Blot makes a nice look at the strip,The results were satisfactory。
Date published: 2017-09-18
Rated 5 out of 5 by from Excellent antibody for FCMFCM analysis of human peripheral blood leukocytes is perfect.
Date published: 2017-07-14
Rated 1 out of 5 by from not very good in IFI used this antibody for IF in liver tissues, 1:40 diluted. Basically no fluorescence under the fluorescent light
Date published: 2017-05-04
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CD163 抗体 (GHI/61) is rated 4.1 out of 5 by 19.
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