Date published: 2025-9-8

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cathepsin B 抗体 (H-5): sc-365558

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说明书
  • cathepsin B 抗体 H-5 是小鼠单克隆 IgG2b κ,cathepsin B抗体, 在55篇文献中引用,规格为200 µg/ml
  • 免疫human物种的代表全长cathepsin B的氨基酸1-339
  • cathepsin B 抗体 (H-5) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 cathepsin B
  • cathepsin B 抗体 (H-5) 可偶联 琼脂糖 用于 IP; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取cathepsin B (H-5): sc-365558免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是cathepsin B Antibody (H-5) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与cathepsin B Antibody (H-5) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。

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関連項目

Cathepsin B抗体(H-5)是一种IgG2b κ小鼠单克隆Cathepsin B抗体(也称为CTSB抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的Cathepsin B蛋白。Cathepsin B抗体(H-5)既可以是非偶联的抗Cathepsin B抗体形式,也可以是多种偶联形式的抗Cathepsin B抗体,包括琼脂糖、PE、FITC和多种Alexa Fluor®偶联物。Cathepsin蛋白酶家族包含多种不同的蛋白酶类。半胱氨酸蛋白酶类包括Cathepsins B、L、H、K、S和O。天冬氨酸蛋白酶类由Cathepsins D和E组成。Cathepsin G属于丝氨酸蛋白酶类。大多数Cathepsins都是溶酶体的,每一种都参与细胞代谢,参与各种事件,如肽的生物合成和蛋白质降解。Cathepsin B表达于腔上皮细胞,表明Cathepsin B是分泌细胞死亡的标志。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

cathepsin B 抗体 (H-5) 参考文献:

  1. 人肺泡巨噬细胞 cathepsin S(一种弹性蛋白分解半胱氨酸蛋白酶)的分子克隆和表达。  |  Shi, GP., et al. 1992. J Biol Chem. 267: 7258-62. PMID: 1373132
  2. 蛋白酶 B 的抑制剂。  |  Frlan, R. and Gobec, S. 2006. Curr Med Chem. 13: 2309-27. PMID: 16918357
  3. 人类 cathepsin D 基因的分子结构。  |  Redecker, B., et al. 1991. DNA Cell Biol. 10: 423-31. PMID: 2069717
  4. Cathepsin B:利用肽底物和抑制剂绘制活性位点图。  |  Schmitz, J., et al. 2019. Bioorg Med Chem. 27: 1-15. PMID: 30473362
  5. 大鼠 cathepsin L 的 cDNA 分子克隆和测序。  |  Ishidoh, K., et al. 1987. FEBS Lett. 223: 69-73. PMID: 3666143
  6. 大鼠 cathepsin H 的 cDNA 分子克隆和测序。  |  Ishidoh, K., et al. 1987. FEBS Lett. 226: 33-7. PMID: 3691815
  7. 细胞凋亡机制中的组织蛋白酶B:执行机制和调节途径。  |  Xie, Z., et al. 2023. Cell Death Dis. 14: 255. PMID: 37031185
  8. 对编码大鼠脾脏胰蛋白酶 E 的两个 cDNA 克隆进行分离和测序,并对纯化的胰蛋白酶 E 的活化进行分析。  |  Okamoto, K., et al. 1995. Arch Biochem Biophys. 322: 103-11. PMID: 7574663
  9. 人 cathepsin O(一种新型内切蛋白酶和兔 OC2 的同源物)的分子克隆。  |  Shi, GP., et al. 1995. FEBS Lett. 357: 129-34. PMID: 7805878
  10. Cathepsin B 是一种与转移进展有关的半胱氨酸蛋白酶,在大鼠前列腺和乳腺退化过程中也有表达。  |  Guenette, RS., et al. 1994. Eur J Biochem. 226: 311-21. PMID: 8001549
  11. 小鼠 cathepsin G 基因的分子克隆, 染色体位置和组织特异性表达。  |  Heusel, JW., et al. 1993. Blood. 81: 1614-23. PMID: 8453108
  12. 小鼠 cathepsin K:cDNA 克隆以及该基因在小鼠发育过程中破骨细胞和某些肥大软骨细胞中的主要表达。  |  Rantakokko, J., et al. 1996. FEBS Lett. 393: 307-13. PMID: 8814310

订购信息

产品名称产品编号规格价格数量收藏夹

cathepsin B 抗体 (H-5)

sc-365558
200 µg/ml
RMB2377.00

cathepsin B (H-5): m-IgGκ BP-HRP 套装

sc-522273
200 µg Ab, 40 µg BP
RMB2662.00

cathepsin B 抗体 (H-5) AC

sc-365558 AC
500 µg/ml, 25% agarose
RMB3129.00

cathepsin B 抗体 (H-5) FITC

sc-365558 FITC
200 µg/ml
RMB2482.00

cathepsin B 抗体 (H-5) PE

sc-365558 PE
200 µg/ml
RMB2580.00

cathepsin B 抗体 (H-5) Alexa Fluor® 488

sc-365558 AF488
200 µg/ml
RMB2685.00

cathepsin B 抗体 (H-5) Alexa Fluor® 546

sc-365558 AF546
200 µg/ml
RMB2685.00

cathepsin B 抗体 (H-5) Alexa Fluor® 594

sc-365558 AF594
200 µg/ml
RMB2685.00

cathepsin B 抗体 (H-5) Alexa Fluor® 647

sc-365558 AF647
200 µg/ml
RMB2685.00

cathepsin B 抗体 (H-5) Alexa Fluor® 680

sc-365558 AF680
200 µg/ml
RMB2685.00

cathepsin B 抗体 (H-5) Alexa Fluor® 790

sc-365558 AF790
200 µg/ml
RMB2685.00

What are the host species?

Asked by: Shirin
Thank you for your question. The host is mouse.
Answered by: BlakeJ
Date published: 2025-04-01

Hello, I would like to use this antibody (cathepsin B antibody (H-5)) for WB and IHC. Can I get a free sample? If so, how long will it take to ship? Now, my research lab is in Toronto. In addition, can this antibody use for IHC (human specimen)? Thanks

Asked by: Ken1228
Thank you for your question. The antibody is recommended for mouse,rat and human samples for WB, IP, IF and ELISA. To request a sample please email [email protected].
Answered by: BlakeJ
Date published: 2024-11-01

To use cathepsin B Antibody for western blot we need to use BSA or milk for dilution?

Asked by: Kanwi
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-12-07

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 13
Date published: 2017-01-17

Hi, I am interested in your new Cathepsin B antibody: sc-365558. Could you please precise the price? Thanks. Sylvie

Asked by: Sylvie31
Thank you for your question. Please contact our European Support office for an accurate quote for this cathepsin B Antibody (H-5): sc-365558. Please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2016-11-30
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Rated 5 out of 5 by from Excellent in WB!Clear and strong signal was detected in WB of SH-SY5Y cells!
Date published: 2024-08-26
Rated 1 out of 5 by from sc-365558 M anti cathepsin B doesn't work in WBRegular WB using rat brain cortex, beta-actin gave a beautiful band with 20 ug protein at 1:1000, cathepsin B in identical blot at 1:1000 didn't work. Tech support on phone was friendly, in email became rude and refuses to provide an antibody replacement despite having provided all employed technical information. Demanding double the protein and a 1:100 antibody application are both completely unreasonable.
Date published: 2023-08-03
Rated 4 out of 5 by from good antibodyThis cathepsin B antibody works great for WB (1/500). 25 kDa band was observed in human liver homogenate .
Date published: 2021-04-19
Rated 5 out of 5 by from Great antibody for human form of Cathepsin-BIt worked great for Human Cathepsin-B bit not for rat.
Date published: 2018-07-09
Rated 4 out of 5 by from 抗体质量很好非常开心得到了cathepsin b抗体的免费试用装,并将它应用到我的实验当中,抗体质量很好,条带很清晰。
Date published: 2018-03-13
Rated 4 out of 5 by from Works for WBIt works for WB 1/200 (overnight 4C in 3%BSA-2%milk). Weak signal for IF (1/50). Hela229 cells.
Date published: 2018-01-12
Rated 5 out of 5 by from Great!I got this free sample and it works very well. I will buy it more.
Date published: 2018-01-02
Rated 2 out of 5 by from It might work with human samples in WBThis Cathepsin B antibody might work with human samples in WB. A 25 kDa band is observed in Hela cells and human liver (not shown in the photo).
Date published: 2017-11-09
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cathepsin B 抗体 (H-5) is rated 4.4 out of 5 by 16.
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