Date published: 2025-9-6

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caspase-7 p20 抗体 (B-5): sc-28295

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说明书
  • caspase-7 p20抗体(B-5)是小鼠单克隆IgG1 κ, 在16篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的caspase-7 p20的N-terminus附近的氨基酸15-50之间
  • 抗-caspase-7 p20 抗体 (B-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 p20 subunit and precursor of caspase-7; 也和以下物种反应,包括: equine
  • 抗caspase-7 p20抗体(B-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取caspase-7 p20 (B-5): sc-28295免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 caspase-7 p20 抗体 (B-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与caspase-7 p20 抗体 (B-5) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

caspase-7 p20抗体(B-5)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过应用诸如蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫 酶联免疫吸附试验(ELISA)。抗半胱天冬酶-7抗体(B-5)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。半胱氨酸蛋白酶-7是半胱氨酸蛋白酶-3/半胱氨酸蛋白酶-1家族的重要成员,该家族是细胞凋亡过程中不可或缺的,通过切割特定的靶蛋白来促进细胞凋亡,这些靶蛋白调节细胞的寿命。半胱氨酸蛋白酶-7的p20亚基在切割聚(ADP-核糖)聚合酶(PARP)中起作用,后者是一种参与DNA修复和细胞应激反应的核蛋白。Caspase-7能够将PARP切割成凋亡片段,这凸显了其在凋亡过程中的重要性,因为这一过程对于维持细胞稳态和防止受损细胞扩散至关重要。Caspase-7由上游caspase(如caspase-10)激活,以响应凋亡信号,这进一步凸显了其在细胞信号通路这一复杂网络中的作用,该网络控制着细胞的死亡和存活。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

caspase-7 p20 抗体 (B-5) 参考文献:

  1. 细胞凋亡过程中自动修饰的聚(ADP-核糖)聚合酶的裂解。Caspase-7 参与其中的证据。  |  Germain, M., et al. 1999. J Biol Chem. 274: 28379-84. PMID: 10497198
  2. 以组成型活性 caspase-7 为诱饵进行酵母双杂交筛选,鉴定 PA28gamma 为效应 caspase 底物。  |  Araya, R., et al. 2002. Cell Death Differ. 9: 322-8. PMID: 11859414
  3. 人类癌症中 CASPASE-7 基因的失活突变。  |  Soung, YH., et al. 2003. Oncogene. 22: 8048-52. PMID: 12970753
  4. Caspase-7 基因干扰揭示了该酶在细胞凋亡早期阶段的参与情况。  |  Korfali, N., et al. 2004. J Biol Chem. 279: 1030-9. PMID: 14583630
  5. 参与细胞凋亡的人类基因 CPP32, Mch2, Mch3 和 Ich-1 的染色体定位。  |  Tiso, N., et al. 1996. Biochem Biophys Res Commun. 225: 983-9. PMID: 8780721
  6. Caspases:细胞凋亡的刽子手。  |  Cohen, GM. 1997. Biochem J. 326 (Pt 1): 1-16. PMID: 9337844
  7. Fas 诱导小鼠肝脏细胞凋亡后,caspase-3 和 caspase-7 的亚细胞分布不同。  |  Chandler, JM., et al. 1998. J Biol Chem. 273: 10815-8. PMID: 9556551
  8. 前列腺癌细胞系 LNCaP 细胞凋亡过程中激活的信号通路:过表达 caspase-7 作为前列腺癌的一种新基因治疗策略。  |  Marcelli, M., et al. 1999. Cancer Res. 59: 382-90. PMID: 9927051

订购信息

产品名称产品编号规格价格数量收藏夹

caspase-7 p20 抗体 (B-5)

sc-28295
200 µg/ml
RMB2377.00

caspase-7 p20 (B-5): m-IgG Fc BP-HRP 套装

sc-526655
200 µg Ab; 10 µg BP
RMB2662.00

caspase-7 p20 (B-5): m-IgG1 BP-HRP 套装

sc-532028
200 µg Ab; 20 µg BP
RMB2662.00

caspase-7 p20 抗体 (B-5) AC

sc-28295 AC
500 µg/ml, 25% agarose
RMB3129.00

caspase-7 p20 抗体 (B-5) HRP

sc-28295 HRP
200 µg/ml
RMB2377.00

caspase-7 p20 抗体 (B-5) FITC

sc-28295 FITC
200 µg/ml
RMB2482.00

caspase-7 p20 抗体 (B-5) PE

sc-28295 PE
200 µg/ml
RMB2580.00

caspase-7 p20 抗体 (B-5) Alexa Fluor® 488

sc-28295 AF488
200 µg/ml
RMB2685.00

caspase-7 p20 抗体 (B-5) Alexa Fluor® 546

sc-28295 AF546
200 µg/ml
RMB2685.00

caspase-7 p20 抗体 (B-5) Alexa Fluor® 594

sc-28295 AF594
200 µg/ml
RMB2685.00

caspase-7 p20 抗体 (B-5) Alexa Fluor® 647

sc-28295 AF647
200 µg/ml
RMB2685.00

caspase-7 p20 抗体 (B-5) Alexa Fluor® 680

sc-28295 AF680
200 µg/ml
RMB2685.00

caspase-7 p20 抗体 (B-5) Alexa Fluor® 790

sc-28295 AF790
200 µg/ml
RMB2685.00

caspase-7 p20 (B-5) 中和勝肽

sc-28295 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-28295 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Western blotThis antibody work well in western blot (gastric cancer cell line: AGS, MKN45, dilute 1:1000)
Date published: 2017-11-14
Rated 5 out of 5 by from high sensitivityThe first thing to strike us was the amazing high specificity, sensitivity, convenience and quickness of the caspase-7 p20 antibody . It is really excellent.
Date published: 2017-10-18
Rated 5 out of 5 by from target signalI used this antibody for WB analysis,I analysis the mouse colon tissue expression of the target protein,I got the target band and no addtional bands seen.
Date published: 2017-07-29
Rated 5 out of 5 by from importThe cysteine protease family is a specific target protein that is critical for the destruction of cell life. And we usually know that polymerase in DNA mutation and double-strand breaks in the measurement plays an indispensable role. This is our test in the oxygen-sliding process important test items
Date published: 2017-02-06
Rated 5 out of 5 by from Amazing IHC(P) stainingcaspase-7 p20 (B-5) gave beautiful IHC(P) staining in human colon tissue showing cytoplasmic and nuclear staining of glandular cells. Also works really well for Western blot (WB).
Date published: 2017-02-03
Rated 5 out of 5 by from Good specificity for IHC(P) stainingGood specificity for IHC(P) staining. -SCBT QC
Date published: 2015-02-04
Rated 5 out of 5 by from Great detection of the p20 cleavage productGreat detection of the p20 cleavage product when HeLa or Jurkat are treated with UV or Staurosporine. -SCBT QC
Date published: 2014-11-12
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caspase-7 p20 抗体 (B-5) is rated 5.0 out of 5 by 7.
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