Date published: 2025-9-7

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caspase-7 抗体 (10-1-62): sc-56063

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说明书
  • caspase-7 抗体 10-1-62 是小鼠单克隆 IgG1 κ,caspase-7抗体, 在34篇文献中引用,规格为200 µg/ml
  • 针对全长caspase-7,human起源
  • caspase-7 抗体 (10-1-62) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 caspase-7, recombinant caspase-7 as well as full length procaspase-7 and processed enzyme
  • 抗caspase-7抗体(10-1-62)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取caspase-7 (10-1-62): sc-56063免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 caspase-7 抗体 (10-1-62) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与caspase-7 抗体 (10-1-62) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

caspase-7抗体(10-1-62)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人源的caspase-7蛋白。 酶联免疫吸附试验(ELISA)。caspase-7抗体(10-1-62)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。半胱氨酸蛋白酶-7(Caspase-7)又称Mch3、ICE-LAP3或CMH-1,是半胱氨酸蛋白酶家族Ced-3/caspase-1的重要成员,该家族是细胞凋亡过程不可或缺的。该家族具有独特的序列、结构和底物特异性,使半胱氨酸蛋白酶-7有别于其他蛋白酶家族。半胱氨酸蛋白酶-7通过切割细胞存活所必需的特定目标蛋白,在细胞凋亡中发挥重要作用,包括参与DNA修复和细胞应激反应的多聚(ADP-核糖)聚合酶(PARP)。半胱氨酸蛋白酶-7切割PARP会产生凋亡片段,促进程序性细胞死亡的进展。Caspase-7由上游caspases(如caspase-10)激活,以响应凋亡信号,这凸显了caspase-7在调节细胞凋亡和细胞稳态中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

caspase-7 抗体 (10-1-62) 参考文献:

  1. 细胞凋亡过程中自动修饰的聚(ADP-核糖)聚合酶的裂解。Caspase-7 参与其中的证据。  |  Germain, M., et al. 1999. J Biol Chem. 274: 28379-84. PMID: 10497198
  2. 以组成型活性 caspase-7 为诱饵进行酵母双杂交筛选,鉴定 PA28gamma 为效应 caspase 底物。  |  Araya, R., et al. 2002. Cell Death Differ. 9: 322-8. PMID: 11859414
  3. 人类癌症中 CASPASE-7 基因的失活突变。  |  Soung, YH., et al. 2003. Oncogene. 22: 8048-52. PMID: 12970753
  4. Caspase-7 基因干扰揭示了该酶在细胞凋亡早期阶段的参与情况。  |  Korfali, N., et al. 2004. J Biol Chem. 279: 1030-9. PMID: 14583630
  5. 在所有乳腺癌中,低裂解的caspase-7水平表明结果不佳。  |  Brentnall, M., et al. 2013. BMC Cell Biol. 14: 32. PMID: 23834359
  6. 表征 Caspase-7 与 RNA 的相互作用。  |  Desroches, A. and Denault, JB. 2021. Biochem J. 478: 2681-2696. PMID: 34156061
  7. Caspase-7激活ASM以修复气门蛋白和穿孔蛋白孔。  |  Nozaki, K., et al. 2022. Nature. 606: 960-967. PMID: 35705808
  8. 凋亡性caspase-7激活通过GSDMB裂解抑制非典型热解。  |  Li, X., et al. 2023. Cell Death Differ. 30: 2120-2134. PMID: 37591921
  9. 参与细胞凋亡的人类基因 CPP32, Mch2, Mch3 和 Ich-1 的染色体定位。  |  Tiso, N., et al. 1996. Biochem Biophys Res Commun. 225: 983-9. PMID: 8780721
  10. Caspases:细胞凋亡的刽子手。  |  Cohen, GM. 1997. Biochem J. 326 (Pt 1): 1-16. PMID: 9337844
  11. Fas 诱导小鼠肝脏细胞凋亡后,caspase-3 和 caspase-7 的亚细胞分布不同。  |  Chandler, JM., et al. 1998. J Biol Chem. 273: 10815-8. PMID: 9556551
  12. 前列腺癌细胞系 LNCaP 细胞凋亡过程中激活的信号通路:过表达 caspase-7 作为前列腺癌的一种新基因治疗策略。  |  Marcelli, M., et al. 1999. Cancer Res. 59: 382-90. PMID: 9927051

订购信息

产品名称产品编号规格价格数量收藏夹

caspase-7 抗体 (10-1-62)

sc-56063
200 µg/ml
RMB2377.00

caspase-7 (10-1-62): m-IgG Fc BP-HRP 套装

sc-526872
200 µg Ab; 10 µg BP
RMB2662.00

caspase-7 (10-1-62): m-IgG1 BP-HRP 套装

sc-532245
200 µg Ab; 20 µg BP
RMB2662.00

caspase-7 抗体 (10-1-62) AC

sc-56063 AC
500 µg/ml, 25% agarose
RMB3129.00

caspase-7 抗体 (10-1-62) HRP

sc-56063 HRP
200 µg/ml
RMB2377.00

caspase-7 抗体 (10-1-62) FITC

sc-56063 FITC
200 µg/ml
RMB2482.00

caspase-7 抗体 (10-1-62) PE

sc-56063 PE
200 µg/ml
RMB2580.00

caspase-7 抗体 (10-1-62) Alexa Fluor® 488

sc-56063 AF488
200 µg/ml
RMB2685.00

caspase-7 抗体 (10-1-62) Alexa Fluor® 546

sc-56063 AF546
200 µg/ml
RMB2685.00

caspase-7 抗体 (10-1-62) Alexa Fluor® 594

sc-56063 AF594
200 µg/ml
RMB2685.00

caspase-7 抗体 (10-1-62) Alexa Fluor® 647

sc-56063 AF647
200 µg/ml
RMB2685.00

caspase-7 抗体 (10-1-62) Alexa Fluor® 680

sc-56063 AF680
200 µg/ml
RMB2685.00

caspase-7 抗体 (10-1-62) Alexa Fluor® 790

sc-56063 AF790
200 µg/ml
RMB2685.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-02-04
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Rated 4 out of 5 by from Good antibody for Western blotWe used a free sample and tested for western in breast cancer cells MCF-7 and trophoblast cells BeWo and we got good results with sharp bands and high specificity.
Date published: 2020-02-14
Rated 5 out of 5 by from 条带清晰cas7的条带还是很容易control,几分钟就显影出来了,这个抗体也好用,没什么杂带,比较清楚,下次还会回购
Date published: 2017-09-19
Rated 5 out of 5 by from Great features!I got free sample several months ago, and I used the recommanded dilution rate ,and the western blot was nice, but I didn't use it in immunohistochemical! Thank you!
Date published: 2017-09-04
Rated 5 out of 5 by from Works well for WBWorks well for WB. Clear distinct bands were observed when used at 1:1000 dilution.
Date published: 2017-07-03
Rated 5 out of 5 by from Great for WB!This monoclonal antibody works well for detection of precursor and cleaved caspase-7 in untreated and Staurosporine treated Jurkat whole cell lysates.
Date published: 2017-01-18
Rated 5 out of 5 by from works well in wbSpecificity is very good useing raw264.7 cell in wb
Date published: 2017-01-16
Rated 5 out of 5 by from Works great for WesternWorks great for Western! Publishable results -SCBT Publication Review
Date published: 2015-02-17
Rated 5 out of 5 by from Good antibody for detecting endogenous proteinGood antibody for detecting endogenous protein -SCBT QC
Date published: 2015-01-21
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caspase-7 抗体 (10-1-62) is rated 4.9 out of 5 by 8.
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