Date published: 2025-9-8
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caspase-1 Antibody (14F468) - Western Blotting - Image 145055
caspase-1 抗体 (14F468): sc-56036. 未经处理 (A), PMA (sc-3576) 和 LPS (sc-3535) 处理 (B) 的 THP-1 全细胞裂解液中 caspase-1 的 Western 印迹分析. 注意 B 道中 caspase-1 的裂解.
caspase-1 Antibody (14F468) - Western Blotting - Image 145055
caspase-1 抗体 (14F468): sc-56036. 未经处理 (A), PMA (sc-3576) 和 LPS (sc-3535) 处理 (B) 的 THP-1 全细胞裂解液中 caspase-1 的 Western 印迹分析. 注意 B 道中 caspase-1 的裂解.
caspase-1 Antibody (14F468) - Western Blotting - Image 17324
procaspase-1 抗体 (14F468): sc-56036. 对 HL-60 (A), LPS 处理的 HL-60 (B) 和 THP-1 (C) 全细胞裂解液中 procaspase-1 的表达进行 Western 印迹分析.
caspase-1 Antibody (14F468) - Western Blotting - Image 395695
caspase-1 抗体 (14F468): sc-56036. 全长人重组 procaspase-1 融合蛋白的 Western 印迹分析.
caspase-1 抗体 (14F468): sc-56036. 未经处理 (A), PMA (sc-3576) 和 LPS (sc-3535) 处理 (B) 的 THP-1 全细胞裂解液中 caspase-1 的 Western 印迹分析. 注意 B 道中 caspase-1 的裂解.

caspase-1 抗体 (14F468): sc-56036

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说明书
  • caspase-1抗体(14F468)是小鼠单克隆IgG1 κ, 在364篇文献中引用，规格为50 µg/0.5 ml
  • 免疫human物种的caspase-1的氨基酸371-390
  • 推荐用于 mouse, rat, human 和 porcine 来源的full-length caspase-1 and cleaved caspase-1 forms that retain amino acids 371-390 of the caspase-1 protein WB, IP, IF 和 IHC(P)检测
  • 看 caspase-1 (D-3): sc-392736，可查看有标记的 caspase-1 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 caspase-1 抗体 (14F468) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与caspase-1 抗体 (14F468) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Caspase 抗体 包括 caspase-1, caspase-10, caspase-11, caspase-12, caspase-14, caspase-2, caspase-3, caspase-4, caspase-5和caspase-6

caspase-1抗体（14F468）是一种小鼠单克隆IgG1 kappa轻链抗体，专门用于检测caspase-1（也称为IL-1转化酶（ICE）），可用于多种应用，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光 （IF）和免疫组织化学。caspase-1 (14F468) 抗体针对人caspase-1的氨基酸区域371-390，能够识别包括小鼠、大鼠、人和猪在内的多种物种的全长和裂解形式。Caspase-1通过蛋白水解作用，催化促炎细胞因子白细胞介素-1β（IL-1β）和白细胞介素-18（IL-18）的非活性前体的成熟，在炎症反应中起着关键作用。此外，caspase-1还参与诱导细胞凋亡，在细胞程序性死亡过程中促进细胞结构解体。活性形式的caspase-1是由p20和p10亚基组成的四聚体，对酶活性至关重要。人类CASP1基因位于2q14染色体上，在肝脏、心脏、骨骼肌、肾脏和睾丸等关键组织中表达。Caspase-1失调与多种病理状况有关，包括炎症、败血性休克、伤口愈合障碍和某些白血病，这凸显了它在正常生理和疾病状态中的重要性。抗Caspase-1抗体（14F468）能够可靠、特异性地检测Caspase-1，有助于研究其在疾病机制中的各种生物学功能和作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

caspase-1 抗体 (14F468) 参考文献:

  1. 同基因缺陷揭示了 Caspases 在细胞凋亡, 发育和细胞因子成熟中的作用。  |  Wang, J. and Lenardo, MJ. 2000. J Cell Sci. 113 (Pt 5): 753-7. PMID: 10671365
  2. caspase-1的前域通过促进caspase-8的活化来增强Fas介导的细胞凋亡。  |  Tatsuta, T., et al. 2000. J Biol Chem. 275: 14248-54. PMID: 10799503
  3. Caspase 途径, 神经细胞凋亡和中枢神经系统损伤。  |  Eldadah, BA. and Faden, AI. 2000. J Neurotrauma. 17: 811-29. PMID: 11063050
  4. 滋养细胞缺氧诱导凋亡机制的蛋白质组学分析。  |  Ishioka, S., et al. 2006. Int J Med Sci. 4: 36-44. PMID: 17299580
  5. Toll-IL-1 受体结构域蛋白 MyD88 适配器样的 NF-kappaB 激活受 Caspase-1 调节。  |  Miggin, SM., et al. 2007. Proc Natl Acad Sci U S A. 104: 3372-7. PMID: 17360653
  6. 炭疽致死毒素通过细胞凋亡或 caspase-1 介导的细胞坏死，以菌株特异性的方式杀死巨噬细胞。  |  Muehlbauer, SM., et al. 2007. Cell Cycle. 6: 758-66. PMID: 17374996
  7. 针对败血症中的 caspase-1：解决老问题的新方法。  |  Matute-Bello, G. 2007. Intensive Care Med. 33: 755-757. PMID: 17384934
  8. Caspase-1自我裂解是终止炎性体活性的内在机制。  |  Boucher, D., et al. 2018. J Exp Med. 215: 827-840. PMID: 29432122
  9. 作为脂质代谢调控分子的 Caspase-1。  |  Molla, MD., et al. 2020. Lipids Health Dis. 19: 34. PMID: 32143623
  10. Caspase-1激活的复杂生物物理之谜  |  Makoni, NJ. and Nichols, MR. 2021. Arch Biochem Biophys. 699: 108753. PMID: 33453207
  11. 纳米二氧化硅颗粒通过ROS/NLRP3/Caspase-1途径诱导热蛋白沉积和心肌肥大  |  Wang, F., et al. 2022. Free Radic Biol Med. 182: 171-181. PMID: 35219847
  12. 白细胞介素-1β转换酶在体内的活性需要寡聚化的加工形式。  |  Gu, Y., et al. 1995. EMBO J. 14: 1923-31. PMID: 7743999

订购信息

产品名称产品编号规格价格数量收藏夹

caspase-1 抗体 (14F468)

sc-56036
50 µg/0.5 ml
RMB2377.00

caspase-1 (14F468): m-IgG Fc BP-HRP 套装

sc-539026
50 µg Ab; 10 µg BP
RMB2662.00

caspase-1 (14F468): m-IgG1 BP-HRP 套装

sc-541214
50 µg Ab; 20 µg BP
RMB2662.00

这款抗体在做免疫组化的时候需要使用抗原修复液吗

Asked by: 曹梦梦1314
感谢您的提问，实验流程可以参考官网：https://www.scbt.com/resources/protocols/immunoperoxidase-staining Thank you for your question, please refer to the online protocol:https://www.scbt.com/resources/protocols/immunoperoxidase-staining. Please contact our Technical Service Department if you have any other questions. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Sen Li
Date published: 2025-08-08

Can this antibody be used to detect both procaspase-1 and caspase-1 p10 proteins of different sizes?

Asked by: chulsoo
Thank you for your question. Yes, this antibody can detect both procaspase-1 and caspase-1 p10 proteins of different sizes.Thank you.
Answered by: Jiajun Z
Date published: 2024-08-13

For western blots, should I dilute the primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 or just T-TBS+5%BSA? Thank you

Asked by: tonytony
Thank you for your question. We recommend UltraCruz® Blocking Reagent: sc-516214 for blocking purposes. In general, milk-based blocking buffers are recommended for reducing background while BSA can be used for improving blots with weak signal.
Answered by: Tech Support Europe
Date published: 2023-07-12

I would be interested in receiving FREE 10 µg sample of caspase-1 (14F468): sc-56036. How can I proceed?

Asked by: Ale68
Thank you for your question. Please reach out to our technical service team directly to arrange for a free sample of this antibody.
Answered by: Tech Support Europe
Date published: 2023-05-25

will alexa fluor 488 or alexa fluor 555 work with this antibody while performing immunofluoroscence of mouse brain tissue?

Asked by: Adnan046
Thank you for your question . Both alexa flour 488 and 555 will work for immunofluoroscence with mouse tissue . Here is a list of secondary antibodies we offer : https://www.scbt.com/browse/Anti-Mouse-IgG-Binding-Protein-Secondaries/_/N-130b1ry.
Answered by: BlakeJ
Date published: 2022-11-17

Hi, I have a question, how many band are revealed with Casp1 antibody (sc-56036)? after my treatment I saw  3 bands (45kDa; 35kDa; 25-27kda) best regards Vincenzo

Asked by: vincenzo81
Thank you for your question. Anti-caspase-1 Antibody (14F468): sc-56036 was raised against amino acids 371-390 of caspase-1 of human origin. As such it is able to recognize any of the five described isoforms for this protein. The bands you are detecting correspond to isoforms alpha, gamma, and delta.
Answered by: Tech Support Europe
Date published: 2024-08-13

What concentration of stimulated THP-1 cells were used for the image on the datasheet?

Asked by: AnonymousII
Thank you for your question. Following our standard protocol, 40–60 µg whole cell lysate of THP-1 cells were used for these western blots. You can find our recommended protocol for western blotting by following this link: https://www.scbt.com/de/resources/protocols/western-immuno-blotting
Answered by: Tech Support Europe
Date published: 2020-06-23

What are the conditions to stimulate THP-1 cells with PMA and LPS to detect CASP1 p10, similar the image of the datasheet? What is the PMA and LPS concentration? How much time?

Asked by: Anonymous
Thank you for your question. Here are the conditions we suggest for stimulation using LPS and PMA, respectively: 5 µg per mL of LPS, induction time 2 hours. 40ng/ml of PMA, induction time 2 hours.
Answered by: Tech Service
Date published: 2020-02-05
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Rated 5 out of 5 by from 抗体特别好用！很好跑！！买来两个月了，实验样本一直没处理好，最近打算跑跑试试，结果接着就跑出来了，抗体真的很好用，推荐推荐！！！
Date published: 2025-02-21
Rated 4 out of 5 by from The effect is very good,The pictures are beautifulThe effect is very good,The effect is very good,The pictures are beautiful
Date published: 2024-11-14
Rated 5 out of 5 by from Detects human procaspase-1 by Western BlotAntibody works great by Western Blot in human spleen (1) and human cell lines THP-1 (3), HL-60 (4) and HL-60 treated with LPS (5), detecting human procaspase-1 -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from nicevery nice，it work well in WB.Good specificity,no background
Date published: 2021-07-31
Rated 4 out of 5 by from WB的条带很明显这个抗体的前体条带特别的明显，但是剪切的条带相对来说比较弱，在不同细胞上都有检测，效果还可以！！！！
Date published: 2020-12-31
Rated 5 out of 5 by from Excellent antibodyIt shows excellent results in western blot, with both pro-caspase-1 and cleaved caspase-1 detected.
Date published: 2020-09-01
Rated 5 out of 5 by from wnt-1抗体非常不错通过代理拿到了wnt-1抗体小样，WB非常不错，条带清晰,没有干扰的咋带。考虑下游通路继续用该公司抗体。
Date published: 2020-07-10
Rated 4 out of 5 by from goodI hope that the product will continue to work well, and the experiment will depend on you later.
Date published: 2019-07-09
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caspase-1 抗体 (14F468) is rated 4.3 out of 5 by 45.
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