Date published: 2025-9-7

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caspase-1 p20 抗体 (D-4): sc-398715

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说明书
  • caspase-1 p20抗体(D-4)是小鼠单克隆IgG2b κ, 在119篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于mouse物种的caspase-1 p20的C-terminus的氨基酸259-294之间
  • 抗-caspase-1 p20 抗体 (D-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse 和rat 来源的 caspase-1 precursor and p20 subunit
  • 抗caspase-1 p20抗体(D-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取caspase-1 p20 (D-4): sc-398715免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 是 caspase-1 p20 抗体 (D-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与caspase-1 p20 抗体 (D-4) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

caspase-1 p20 抗体(D-4)是一种 IgG2b κ 小鼠单克隆 caspase-1 p20 抗体,可通过 WB、IP、IF、IHC(P) 和 ELISA 检测到小鼠和大鼠来源的 caspase-1 前体和 p20 亚基。caspase-1 p20 抗体(D-4)既可以是非偶联抗 caspase-1 p20 抗体形式,也可以是多种偶联形式的抗 caspase-1 p20 抗体,包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。Caspase-1 最初被命名为 ICE(IL-1 转换酶),是具有大前结构域的 caspase 家族成员之一。Caspase-1 通过蛋白水解切割前体形式,促进白细胞介素 IL-1β 和白细胞介素 18(IL-18)的成熟,将其转化为具有生物活性的促炎细胞因子。Caspase-1 的前结构域(也称为 Pro-C1)代表 caspase-1 前体的氨基酸末端部分。Pro-C1 是前体蛋白水解切割后产生的残余组分,生成具有功能的 caspase-1 亚基,即 p20 和 p10 亚基。活性 caspase-1 是一种 (p20/p10)2 四聚体,对于前体 IL-1 的切割以及在某些细胞系中诱导凋亡是必要且充分的。高度保守的 caspase 家族介导凋亡的许多形态学和生化特征,包括细胞体和细胞核的结构解体、基因组 DNA 的片段化、调节蛋白的降解以及其他促凋亡分子的传播。人类 Caspase-1 基因位于 2 号染色体 2q14 区域,编码一种在肝脏、心脏、骨骼肌肾脏和睾丸中表达的细胞质蛋白。Caspase-1 涉及炎症、感染性休克等多种生理病理过程,如伤口愈合和某些白血病的生长。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

caspase-1 p20 抗体 (D-4) 参考文献:

  1. 猫科动物和犬科动物冰相关 cDNA 的克隆和测序,编码混合型 caspase-1/caspase-13 样肽。  |  Taylor, S., et al. 2000. DNA Seq. 10: 387-94. PMID: 10826695
  2. 最残忍的一刀:哺乳动物 Caspases 的多重作用。  |  Fadeel, B., et al. 2000. Leukemia. 14: 1514-25. PMID: 10942252
  3. 细胞凋亡中的非天冬酶蛋白酶  |  Johnson, DE. 2000. Leukemia. 14: 1695-703. PMID: 10995018
  4. Caspase 途径, 神经细胞凋亡和中枢神经系统损伤。  |  Eldadah, BA. and Faden, AI. 2000. J Neurotrauma. 17: 811-29. PMID: 11063050
  5. 细胞自杀的蛋白酶:caspases 的功能和调控。  |  Chang, HY. and Yang, X. 2000. Microbiol Mol Biol Rev. 64: 821-46. PMID: 11104820
  6. Caspases:细胞程序性死亡的关键角色。  |  Grütter, MG. 2000. Curr Opin Struct Biol. 10: 649-55. PMID: 11114501
  7. Caspases 的信号:它们在前列腺细胞凋亡中的作用。  |  Coffey, RN., et al. 2001. J Urol. 165: 5-14. PMID: 11125352
  8. 胰腺腺癌和慢性胰腺炎中 Caspase-1 的过度表达。  |  Yang, YM., et al. 2003. World J Gastroenterol. 9: 2828-31. PMID: 14669344
  9. 白细胞介素-1β转换酶前体的表达, 重折叠和自催化蛋白水解处理。  |  Ramage, P., et al. 1995. J Biol Chem. 270: 9378-83. PMID: 7721861

订购信息

产品名称产品编号规格价格数量收藏夹

caspase-1 p20 抗体 (D-4)

sc-398715
200 µg/ml
RMB2377.00

caspase-1 p20 (D-4): m-IgG Fc BP-HRP 套装

sc-526321
200 µg Ab; 10 µg BP
RMB2662.00

caspase-1 p20 抗体 (D-4) AC

sc-398715 AC
500 µg/ml, 25% agarose
RMB3129.00

caspase-1 p20 抗体 (D-4) HRP

sc-398715 HRP
200 µg/ml
RMB2377.00

caspase-1 p20 抗体 (D-4) FITC

sc-398715 FITC
200 µg/ml
RMB2482.00

caspase-1 p20 抗体 (D-4) PE

sc-398715 PE
200 µg/ml
RMB2580.00

caspase-1 p20 抗体 (D-4) Alexa Fluor® 488

sc-398715 AF488
200 µg/ml
RMB2685.00

caspase-1 p20 抗体 (D-4) Alexa Fluor® 546

sc-398715 AF546
200 µg/ml
RMB2685.00

caspase-1 p20 抗体 (D-4) Alexa Fluor® 594

sc-398715 AF594
200 µg/ml
RMB2685.00

caspase-1 p20 抗体 (D-4) Alexa Fluor® 647

sc-398715 AF647
200 µg/ml
RMB2685.00

caspase-1 p20 抗体 (D-4) Alexa Fluor® 680

sc-398715 AF680
200 µg/ml
RMB2685.00

caspase-1 p20 抗体 (D-4) Alexa Fluor® 790

sc-398715 AF790
200 µg/ml
RMB2685.00

caspase-1 p20 (D-4) 中和勝肽

sc-398715 P
100 µg/0.5 ml
RMB511.00

What is the expected molecular weight of the protein detected by this antibody, caspase-1 p20 (D-4): sc-398715?

Asked by: TinTin
This antibody, caspase-1 p20: sc-398715, is recommended for the detection of caspase-1 precursor and p20 subunit. The expected molecular weights of these caspase forms are ~45kDa and ~20kDa, respectively, however detection of the p20 subunit can be difficult to observe by Western blot, so it is more common to only see the precursor band.
Answered by: Technical Support 12
Date published: 2023-12-04

What application is the blocking peptide sc-398715 P appropriate for?

Asked by: jenni
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Service 11
Date published: 2019-05-25
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Rated 5 out of 5 by from 条带清晰,背景底我对这个抗体感到非常满意,价格优惠,希望santa cruz公司推出更多优秀的抗体,为全球科学家提供更多有价值的产品,做出更多的贡献。
Date published: 2021-12-22
Rated 5 out of 5 by from 功能出色同事订购过此产品caspase-1 p20抗体 (D-4),使用效果不错,条带清晰,我希望可以获取小样试用,将会非常高兴。
Date published: 2018-05-11
Rated 5 out of 5 by from IHCUsed it for IHC got very perfect staining for mouse colon section.1:150 very good concetration.
Date published: 2018-01-27
Rated 1 out of 5 by from Caspase-1 p20 antibodyI received a sample size to test for active caspase-1 in the medium of BMDCs stimulated in various conditions. I wasn't able to detect any bands at all. :(
Date published: 2018-01-06
Rated 5 out of 5 by from Good!Caspase 1 p-20(d-4) protein: from mouse liver extracted with RIPA Loading 20ug/well
Date published: 2017-12-12
Rated 5 out of 5 by from excellent qualityA good staining was obtained in rat colon tissue in IHC(1:100 dilution) and very good antibodies were obtained.
Date published: 2017-10-26
Rated 5 out of 5 by from 功能很好上次申请的小样试用装非常好用,它不仅能曝出全长的caspase 1蛋白条带,还能曝出caspase-1 p20条带。但是就是还有些扎蛋白条带。不过都不影响条带。
Date published: 2017-10-15
Rated 3 out of 5 by from Trial Report sc-398715I bought this antibody(sc-398715) a month ago and it's pity that there was no flaser. Maybe the protein I dectected was not form the model organism-mouse so there are many different amino acid sequences in this protein.
Date published: 2017-09-25
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caspase-1 p20 抗体 (D-4) is rated 4.0 out of 5 by 29.
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