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CaMKIV Antibody (A-3) - Western Blotting - Image 73140
CaMKIV 抗体 (A-3): sc-166156. 拉莫斯 (A) 和 Jurkat (B) 全细胞裂解液中 CaMKIV 表达的 Western 印迹分析.
CaMKIV 抗体 (A-3): sc-166156. 拉莫斯 (A) 和 Jurkat (B) 全细胞裂解液中 CaMKIV 表达的 Western 印迹分析.

CaMKIV 抗体 (A-3): sc-166156

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说明书
  • CaMKIV抗体(A-3)是小鼠单克隆IgG2b κ, 在5篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的CaMKIV的C-terminus的氨基酸423-461之间
  • CaMKIV 抗体 (A-3) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 CaMKIV and Calspermin
  • 抗CaMKIV抗体（A-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CaMKIV (A-3): sc-166156的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 CaMKIV 抗体 (A-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CaMKIV 抗体 (A-3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 CaMK 抗体 包括 CaMKI, CaMKI δ, CaMKI γ, CaMKII, CaMKII α, CaMKII β, CaMKII δ, CaMKII γ, CaMKIV和CaMKK α

CaMKIV抗体（A-3）是一种IgG2b κ小鼠单克隆CaMKIV抗体（也称为CaMKIV抗体或CAMK4抗体），可通过WB、IP、IF、IHC(P)和ELISA检测人类来源的CaMKIV和Calspermin。CaMKIV抗体（A-3）既有非偶联形式的抗CaMKIV抗体，也有多种偶联形式的抗CaMKIV抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。钙/钙调蛋白依赖性蛋白激酶（CaM激酶）是结构相关的丝氨酸/苏氨酸激酶亚家族，包括CaMKI、CaMKII和CaMKIV。CaMKII是一种普遍表达的丝氨酸/苏氨酸蛋白激酶，由钙离子和钙调蛋白（CaM）激活，参与细胞周期和转录的调节。CaMKII有四种同功酶，分别为α、β、γ和δ，它们可能在同一组织类型中共同表达，也可能不共同表达。CaMKIV由钙离子和钙调蛋白刺激，但也需要CaMK的磷酸化才能完全激活。用抗CD3单克隆抗体刺激T细胞受体CD3信号复合物，可使CaMKIV活性增加10-40倍。此外，还有一种激酶CaMKK，它通过特异性磷酸化位于177位的调节性苏氨酸残基来激活CaMKI。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CaMKIV 抗体 (A-3) 参考文献:

  1. CaMKIV 对成年小鼠受伤和恐惧诱发的超声波发声的贡献  |  Ko, SW., et al. 2005. Mol Pain. 1: 10. PMID: 15813959
  2. ER81和CaMKIV确定了小鼠嗅球中间神经元的解剖学和表型。  |  Saino-Saito, S., et al. 2007. J Comp Neurol. 502: 485-96. PMID: 17394138
  3. CaMKIV参与慢性氟西汀治疗的神经源效应  |  Song, N., et al. 2013. Int J Neuropsychopharmacol. 16: 803-12. PMID: 22695008
  4. 刺激 Sigma-1 受体可改善 CaMKIV 缺失小鼠的抑郁样行为  |  Moriguchi, S., et al. 2015. Mol Neurobiol. 52: 1210-1222. PMID: 25316382
  5. camKK2/camKIV 中继是肝癌的重要调节因子。  |  Lin, F., et al. 2015. Hepatology. 62: 505-20. PMID: 25847065
  6. 探索 CaMKIV 在同态可塑性中的作用  |  Bleier, J. and Toliver, A. 2017. J Neurosci. 37: 11520-11522. PMID: 29187612
  7. 钙/钙调蛋白依赖性蛋白激酶 IV（CaMKIV）的活化通过抑制肠上皮细胞增殖而促进了实验性结肠炎的发病机制。  |  Cunningham, KE., et al. 2019. FASEB J. 33: 1330-1346. PMID: 30113881
  8. 七氟烷通过抑制Wnt/β-Catenin/CaMKIV通路诱导新生大鼠海马小胶质细胞发炎  |  Wang, F., et al. 2021. J Pharmacol Sci. 146: 105-115. PMID: 33941321
  9. Nrf2通过circ-Vps41/miR-26a-5p/CaMKIV调控网络改善海马突触可塑性、学习和记忆。  |  Zhang, R., et al. 2022. Exp Neurol. 351: 113998. PMID: 35143833
  10. 在先天性甲状腺功能减退症中，CaMKIV通过调节EGR3/BDNF信号轴介导海马神经元脊柱生长缺陷。  |  Wu, H., et al. 2022. Cell Death Discov. 8: 482. PMID: 36473844

订购信息

产品名称产品编号规格价格数量收藏夹

CaMKIV 抗体 (A-3)

sc-166156
200 µg/ml
RMB2377.00

CaMKIV (A-3): m-IgGκ BP-HRP 套装

sc-521524
200 µg Ab, 40 µg BP
RMB2662.00

CaMKIV (A-3): m-IgG2b BP-HRP 套装

sc-548920
200 µg Ab; 10 µg BP
RMB2662.00

CaMKIV 抗体 (A-3) AC

sc-166156 AC
500 µg/ml, 25% agarose
RMB3129.00

CaMKIV 抗体 (A-3) HRP

sc-166156 HRP
200 µg/ml
RMB2377.00

CaMKIV 抗体 (A-3) FITC

sc-166156 FITC
200 µg/ml
RMB2482.00

CaMKIV 抗体 (A-3) PE

sc-166156 PE
200 µg/ml
RMB2580.00

CaMKIV 抗体 (A-3) Alexa Fluor® 488

sc-166156 AF488
200 µg/ml
RMB2685.00

CaMKIV 抗体 (A-3) Alexa Fluor® 546

sc-166156 AF546
200 µg/ml
RMB2685.00

CaMKIV 抗体 (A-3) Alexa Fluor® 594

sc-166156 AF594
200 µg/ml
RMB2685.00

CaMKIV 抗体 (A-3) Alexa Fluor® 647

sc-166156 AF647
200 µg/ml
RMB2685.00

CaMKIV 抗体 (A-3) Alexa Fluor® 680

sc-166156 AF680
200 µg/ml
RMB2685.00

CaMKIV 抗体 (A-3) Alexa Fluor® 790

sc-166156 AF790
200 µg/ml
RMB2685.00

CaMKIV (A-3) 中和勝肽

sc-166156 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-166156 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Excellent antibody: All in One!CaMK-IV (SC166156) is an excellent antibody and it will help you to paint your blot with clear band as well as spot the protein in the crowded intra-cellular environment (Immuno-fluorescence detection). I just purchased this antibody to use it for immunoblot and for Immuno-fluorescence study in human cell line. In Immunoblot it gives a clear band at 55kDa which is the right molecular weight and match perfectly with theoretical mol. weight prediction (see the attached blots). However, do not get disappointed if you see occasional high mol. weight band (as in the 2nd blot), which definitely is post translational modification (can't disclose further) and if you explore it may lead to great discovery. In immuno-fluorescence it showed clear localization in the cytoplasm, as well as in the nucleus and nucleolus, the nucleolar localization is very distinct and other scientist reported this. So if you are looking for a multipurpose CaMK-IV antibody then this is the ONE. Go for it and do great science.
Date published: 2017-05-05
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmicProduced beautiful immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human duodenum tissue. -SCBT QC
Date published: 2015-03-13
Rated 5 out of 5 by from Good specificity in WB in 293T whole cell lysateGood specificity in WB in 293T whole cell lysate. -SCBT QC
Date published: 2015-03-07
Rated 5 out of 5 by from Strong band in WB in human CaMKIV transfectedStrong band in WB in human CaMKIV transfected 293 whole cell lysate. -SCBT QC
Date published: 2015-03-03
Rated 5 out of 5 by from GoodGood, specific band seen in WB in Ramos and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-03-02
Rated 5 out of 5 by from Strong band in WB in human CaMKIV transfectedStrong band in WB in human CaMKIV transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-02-17
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CaMKIV 抗体 (A-3) is rated 5.0 out of 5 by 6.
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