Date published: 2025-9-6

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CaMKIV 抗体 (H-5): sc-55501

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说明书
  • CaMKIV 抗体 H-5 是小鼠单克隆 IgG1 κ,CaMKIV抗体, 在13篇文献中引用,规格为200 µg/ml
  • 免疫human来源CaMKIV位于C-terminus 的328-473氨基酸
  • CaMKIV 抗体 (H-5) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 CaMKIV
  • 抗CaMKIV抗体(H-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CaMKIV (H-5): sc-55501免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是CaMKIV Antibody (H-5) for WB applications. 的首选辅助检测试剂,这些试剂现与CaMKIV Antibody (H-5) 打包提供(请参阅下面的订购信息)。

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関連項目

CaMKIV 抗体(H-5)是一种小鼠单克隆 IgG1 kappa 轻链抗体,可通过免疫印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附试验(ELISA)检测人源 CaMKIV 蛋白。抗 CaMKIV 抗体 (H-5) 有非共轭型和多种共轭型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。CaMKIV 在细胞信号通路中发挥着至关重要的作用,尤其是在基因表达和 T 细胞活化的调控中。CaMKIV 主要位于细胞核中,在细胞核中,CaMKIV 磷酸化转录因子,从而影响参与免疫反应和神经元功能的基因转录。CaMKIV 的核定位对其功能至关重要,因为这种定位允许它与调控基因表达的靶蛋白直接相互作用并对其进行修饰。CaMKIV 由钙离子和钙调素激活,CaMKIV 的活性可通过其他激酶(如 CaMKK)的磷酸化而显著增强。这种错综复杂的调控强调了 CaMKIV 在介导细胞对钙信号反应中的重要性,使 CaMKIV 成为免疫和神经元信号通路中的一个重要组成部分。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CaMKIV 抗体 (H-5) 参考文献:

  1. 导入蛋白α将 CaMKIV 运送到细胞核,而不需要利用导入蛋白β。  |  Kotera, I., et al. 2005. EMBO J. 24: 942-51. PMID: 15719015
  2. CaMKIV KO 小鼠的眼动条件反射记忆受损  |  Lee, KH., et al. 2009. Behav Neurosci. 123: 438-42. PMID: 19331466
  3. 抑制 CaMKIV 可通过调节 HMGB1 缓解链脲佐菌素诱导的糖尿病神经病理性疼痛  |  Zhao, X., et al. 2016. BMC Anesthesiol. 16: 27. PMID: 27216039
  4. 镁通过增加 OPN 的表达和激活 CaM/CaMKIV/CREB1 通路促进成骨。  |  Hou, P., et al. 2022. J Biomed Mater Res B Appl Biomater. 110: 1594-1603. PMID: 35106922
  5. 链脲佐菌素诱导的糖尿病小鼠前额叶皮层、杏仁核、海马和下丘脑中 CaMKIV/CREB/BDNF 信号通路的表达与焦虑样行为。  |  Espasandín, C., et al. 2022. Exp Brain Res. 240: 2687-2699. PMID: 35984483
  6. 对精子中与 CaMKIV 共定位的 CaMKKβ/2 剪接变体进行转录物、生物化学和免疫组织化学分析。  |  Ohtsuka, S., et al. 2024. Cell Calcium. 117: 102820. PMID: 37979343
  7. 心脏肌浆网磷酰班激酶是一种独特的 delta-CaM 激酶同工酶。  |  Baltas, LG., et al. 1995. FEBS Lett. 373: 71-5. PMID: 7589437
  8. 钙离子/钙调蛋白依赖性蛋白激酶级联的特征。钙/钙调蛋白依赖性蛋白激酶激酶的分子克隆和表达。  |  Tokumitsu, H., et al. 1995. J Biol Chem. 270: 19320-4. PMID: 7642608
  9. 钙/钙调蛋白依赖性蛋白激酶II能下调人类T细胞中钙调素和蛋白激酶C介导的细胞因子基因转录途径。  |  Hama, N., et al. 1995. J Exp Med. 181: 1217-22. PMID: 7869038
  10. KN-93 是 CaMK-II(多功能 Ca2+/CaM 激酶)的一种阻抑剂,可诱导 NIH 3T3 细胞的 G1 细胞周期停滞和细胞凋亡。  |  Tombes, RM., et al. 1995. Cell Growth Differ. 6: 1063-70. PMID: 8519682
  11. 过表达 Ca2+/calmodulin 依赖性蛋白激酶 II 可抑制 PC12 细胞的神经元生长。  |  Tashima, K., et al. 1996. J Neurochem. 66: 57-64. PMID: 8522989
  12. Jurkat T 淋巴细胞中 CaM 激酶对 Ca2+/calmodulin 依赖性蛋白激酶(CaM-激酶)IV 的激活。  |  Park, IK. and Soderling, TR. 1995. J Biol Chem. 270: 30464-9. PMID: 8530476

订购信息

产品名称产品编号规格价格数量收藏夹

CaMKIV 抗体 (H-5)

sc-55501
200 µg/ml
RMB2377.00

CaMKIV (H-5): m-IgG Fc BP-HRP 套装

sc-526858
200 µg Ab; 10 µg BP
RMB2662.00

CaMKIV (H-5): m-IgG1 BP-HRP 套装

sc-532231
200 µg Ab; 20 µg BP
RMB2662.00

CaMKIV 抗体 (H-5) AC

sc-55501 AC
500 µg/ml, 25% agarose
RMB3129.00

CaMKIV 抗体 (H-5) HRP

sc-55501 HRP
200 µg/ml
RMB2377.00

CaMKIV 抗体 (H-5) FITC

sc-55501 FITC
200 µg/ml
RMB2482.00

CaMKIV 抗体 (H-5) PE

sc-55501 PE
200 µg/ml
RMB2580.00

CaMKIV 抗体 (H-5) Alexa Fluor® 488

sc-55501 AF488
200 µg/ml
RMB2685.00

CaMKIV 抗体 (H-5) Alexa Fluor® 546

sc-55501 AF546
200 µg/ml
RMB2685.00

CaMKIV 抗体 (H-5) Alexa Fluor® 594

sc-55501 AF594
200 µg/ml
RMB2685.00

CaMKIV 抗体 (H-5) Alexa Fluor® 647

sc-55501 AF647
200 µg/ml
RMB2685.00

CaMKIV 抗体 (H-5) Alexa Fluor® 680

sc-55501 AF680
200 µg/ml
RMB2685.00

CaMKIV 抗体 (H-5) Alexa Fluor® 790

sc-55501 AF790
200 µg/ml
RMB2685.00

What is the recommended fixation method for immunofluorescence staining with CaMKIV (H-5): sc-55501 monoclonal antibody?

Asked by: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from 免疫染色マウス単離細胞に対して蛍光免疫染色を行いました。多少ノンスペがありましたが、良好な染色画像を得ました。
Date published: 2018-11-12
Rated 4 out of 5 by from Good AntibodyI tested this antibody on mouse brain lysate, detected single protein band in WB (1:200 dilution).
Date published: 2018-07-09
Rated 5 out of 5 by from what we needThe main ingredient of this product, CaMKII, is a widely expressed serine / threonine protein kinase, which is involved in the regulation of cell cycle and transcription by calmodulin activation. We think that this feature is what we need
Date published: 2017-02-09
Rated 5 out of 5 by from Great antibodyCaMKIV Antibody (H-5) produced nice clear bands and beautiful staining by IHCP and IF
Date published: 2017-01-25
Rated 5 out of 5 by from Good band in WB in human CaMKIV transfectedGood band in WB in human CaMKIV transfected 293 whole cell lysate. -SCBT QC
Date published: 2015-03-11
Rated 4 out of 5 by from Large band seen in WB in human CaMKIV transfectedLarge band seen in WB in human CaMKIV transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-01-17
Rated 5 out of 5 by from Great specificity in WB in Jurkat whole cellGreat specificity in WB in Jurkat whole cell lysate. -SCBT QC
Date published: 2013-05-09
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CaMKIV 抗体 (H-5) is rated 4.6 out of 5 by 7.
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