Date published: 2025-9-9
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Calnexin Antibody (E-10) - Western Blotting - Image 352813
Calnexin抗体 (E-10): sc-46669. NCI-H1299 (A), A549 (B), K-562 (C), MCF7 (D) 和 HeLa (E) 全细胞裂解液中 Calnexin 表达的 Western 印迹分析.
Calnexin抗体 (E-10): sc-46669. NCI-H1299 (A), A549 (B), K-562 (C), MCF7 (D) 和 HeLa (E) 全细胞裂解液中 Calnexin 表达的 Western 印迹分析.

Calnexin 抗体 (E-10): sc-46669

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寫評論提問

说明书
  • Calnexin抗体(E-10)是小鼠单克隆IgG2a κ, 在81篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Calnexin的氨基酸1-70
  • 抗-Calnexin 抗体 (E-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Calnexin
  • 抗Calnexin抗体（E-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Calnexin (E-10): sc-46669的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 Calnexin 抗体 (E-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Calnexin 抗体 (E-10) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Calnexin抗体（E-10）是一种IgG2a κ小鼠单克隆Calnexin抗体（也称为CANX抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的Calnexin。Calnexin抗体（E-10）有非偶联的抗Calnexin抗体形式，以及多种偶联形式的抗Calnexin抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Calnexin和Calregulin（也称为calreticulin）是定位于内质网的钙结合蛋白，其中Calnexin定位于膜上，而Calregulin定位于内腔。Calnexin是一种I型膜蛋白，在内质网中与新合成的糖蛋白相互作用。它可能在协助蛋白质组装和将未组装的蛋白质亚基保留在内质网中发挥作用。Calregulin具有低亲和力和高亲和力的钙结合位点。Calnexin和Calregulin都不含在钙调蛋白中发现的钙结合"E-F手"基序。Calnexin和Calregulin对于内质网中糖蛋白的成熟非常重要，并且似乎与许多相同的蛋白质结合。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Calnexin 抗体 (E-10)

sc-46669
200 µg/ml
RMB2377.00

Calnexin (E-10): m-IgG Fc BP-HRP 套装

sc-528496
200 µg Ab; 10 µg BP
RMB2662.00

Calnexin (E-10): m-IgGκ BP-HRP 套装

sc-520888
200 µg Ab, 40 µg BP
RMB2662.00

Calnexin (E-10): m-IgG2a BP-HRP 套装

sc-547108
200 µg Ab; 10 µg BP
RMB2662.00

Calnexin 抗体 (E-10) AC

sc-46669 AC
500 µg/ml, 25% agarose
RMB3129.00

Calnexin 抗体 (E-10) HRP

sc-46669 HRP
200 µg/ml
RMB2377.00

Calnexin 抗体 (E-10) FITC

sc-46669 FITC
200 µg/ml
RMB2482.00

Calnexin 抗体 (E-10) PE

sc-46669 PE
200 µg/ml
RMB2580.00

Calnexin 抗体 (E-10) Alexa Fluor® 488

sc-46669 AF488
200 µg/ml
RMB2685.00

Calnexin 抗体 (E-10) Alexa Fluor® 546

sc-46669 AF546
200 µg/ml
RMB2685.00

Calnexin 抗体 (E-10) Alexa Fluor® 594

sc-46669 AF594
200 µg/ml
RMB2685.00

Calnexin 抗体 (E-10) Alexa Fluor® 647

sc-46669 AF647
200 µg/ml
RMB2685.00

Calnexin 抗体 (E-10) Alexa Fluor® 680

sc-46669 AF680
200 µg/ml
RMB2685.00

Calnexin 抗体 (E-10) Alexa Fluor® 790

sc-46669 AF790
200 µg/ml
RMB2685.00

tsg101(C-2):sc7964和Calnexin(E10):sc-46669，这两个抗体跑western blot的时候一定需要用配套来的marker吗？cruz marker TM MW Tag和Cruz Marker TM Molecular Weight Standards如何使用？说明书看得有点蒙

Asked by: dengdeng
感谢您的提问。您可以拨打电话：021-60936350，发送邮件到[email protected]，或者我们官网上的在线聊天，联系我们的亚洲技术支持团队，了解更多信息。 Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Service SH
Date published: 2022-12-02

Can Calnexin (E-10): sc-46669 be frozen?

Asked by: jenniferc
No. The antibody solution contains gelatin. When the gelatin is frozen the antibody precipitates out of solution. However, if an antibody’s datasheet says that the antibody should be stored frozen, then the antibody can be frozen, as these antibodies do not contain gelatin.
Answered by: Technical Support 9
Date published: 2017-01-24
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Rated 4 out of 5 by from Good for identifying the endogenous protein.Although not strong enough to be used as a loading control, it was good for detecting endogenous proteins. I used it on H9c2, a rat cardiomyoblast, at 1:1000.
Date published: 2024-02-06
Rated 5 out of 5 by from IF so clear to show ER spaceI tried stain ER space by calnexin, and it works well. The clear figure is in Supplemental Fig 2 (PMID: 36548354).
Date published: 2022-12-26
Rated 5 out of 5 by from Good for IFworked very good and showed specificity in immunofluorescent staining (x400 RT 1hr, HCHO fix, HeLa S3)
Date published: 2022-08-07
Rated 5 out of 5 by from 1:1000 IF stainingworked very good and showed specificity in immunofluorescent staining
Date published: 2021-06-25
Rated 5 out of 5 by from Excellent in WB used as loading controlWestern Blottin Dilution 1:1000 Hunam cell lysate (CFBE) Stron and specific band at teh expected molecular weight
Date published: 2021-04-16
Rated 5 out of 5 by from Wonderful antibody, won't give you any troubleExcellent antibody, if you do not get your band/result then it's your fault. Highly recommended.
Date published: 2020-09-04
Rated 5 out of 5 by from This antibody very work with western blotThis antibody is very specific with western blot with SAS cell lysate.
Date published: 2020-06-12
Rated 5 out of 5 by from worked great for IFworked great for IF at 1:200 for murine macrophages.
Date published: 2020-03-12
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Calnexin 抗体 (E-10) is rated 4.9 out of 5 by 18.
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