Date published: 2025-9-6
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Myc Antibody (C-33) - Western Blotting - Image 349919
c-Myc 抗体 (C-33): sc-42. HL-60 全细胞裂解液中 c-Myc 表达的 Western 印迹分析.
c-Myc 抗体 (C-33): sc-42. HL-60 全细胞裂解液中 c-Myc 表达的 Western 印迹分析.

Myc 抗体 (C-33): sc-42

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说明书
  • Myc抗体(C-33)是小鼠单克隆IgG1 κ, 在346篇文献中引用，规格为200 µg/ml
  • 针对全长c-Myc，human起源
  • 抗-Myc 抗体 (C-33) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat, human 和avian 来源的 c-Myc p67 and c-Myc tagged fusion proteins
  • 抗Myc抗体（C-33）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-42 X, 200 µg/0.1 ml)
  • 关于如何获取Myc (C-33): sc-42的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Myc Antibody (C-33) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Myc Antibody (C-33) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Myc抗体，包括c-Myc和N-Myc

c-Myc 抗体（C-33）是一种针对人类 c-Myc 的小鼠单克隆 IgG1 kappa 轻链抗体。c-Myc 小鼠单克隆抗体（C-33）可识别小鼠、大鼠和人类 c-Myc p67 蛋白和 c-Myc 标记融合蛋白中的表位。抗c-Myc单克隆抗体（C-33）在蛋白质印迹、免疫沉淀、免疫荧光和酶联免疫吸附试验（ELISA）应用中表现出很高的有效性。c-Myc（C-33）抗体有非结合型和多种结合型，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®结合型，可满足各种检测需求。c-Myc蛋白由8q24染色体上的MYC原癌基因编码，在细胞周期进展、细胞凋亡和细胞转化中发挥关键转录因子的作用。作为Myc家族转录因子的成员，c-Myc包含多个保守结构域，包括位于C端的碱性螺旋-环-螺旋-亮氨酸拉链（bHLH-LZ）结构域，该结构域对于DNA结合和与Max蛋白的二聚化至关重要。c-Myc可调控参与核糖体生物合成、代谢、蛋白质合成和细胞生长的众多靶基因。c-Myc基因的过度表达或扩增通常发生在各种人类癌症中，包括伯基特淋巴瘤、神经母细胞瘤以及肺癌、乳腺癌、结肠癌和宫颈癌。c-Myc的失调会破坏正常的细胞稳态，导致细胞无序增殖和分化抑制。在某些条件下，c-Myc可以诱导细胞凋亡，作为防止肿瘤形成的故障安全机制；然而，额外的致癌信号或肿瘤抑制因子功能的丧失可以绕过这种凋亡途径。c-Myc与另一个bHLH-LZ蛋白Max形成异源二聚体，结合靶基因启动子中的特定E-box DNA序列（CACGTG），调节其转录。c-Myc/Max复合物作为转录激活因子发挥作用，而Max也可以与Mad1和Mxi1等其他蛋白形成异源二聚体，从而抑制转录。这种激活与抑制之间的动态相互作用维持了正常的细胞功能。鉴于c-Myc在细胞生物学和癌症中的核心作用，c-Myc仍然是癌症研究的关键目标。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Myc 抗体 (C-33) 参考文献:

  1. c-Myc上调的肿瘤细胞中的转录扩增。  |  Lin, CY., et al. 2012. Cell. 151: 56-67. PMID: 23021215
  2. c-MYC诱导的基因组不稳定性。  |  Kuzyk, A. and Mai, S. 2014. Cold Spring Harb Perspect Med. 4: a014373. PMID: 24692190
  3. c-MYC和上皮性卵巢癌。  |  Reyes-González, JM. and Vivas-Mejía, PE. 2021. Front Oncol. 11: 601512. PMID: 33718147
  4. 一种选择性小分子 c-Myc 降解剂能有效抑制致命的 c-Myc 过表达肿瘤  |  Xu, Y., et al. 2022. Adv Sci (Weinh). 9: e2104344. PMID: 35048559
  5. c-Myc-IMPDH1/2轴通过调节GTP代谢重编程促进肿瘤发生。  |  Zhang, Q., et al. 2023. Clin Transl Med. 13: e1164. PMID: 36629054
  6. 铜蓝蛋白病与c-Myc介导的乳腺癌干细胞性有关。  |  Wang, R., et al. 2023. J Transl Med. 21: 409. PMID: 37353799
  7. DUBA-SLC7A11-c-Myc轴对干细胞和铁死亡至关重要。  |  Wang, Z., et al. 2023. Oncogene. 42: 2688-2700. PMID: 37537342
  8. c-Myc抑制B细胞淋巴瘤中的LAPTM5表达。  |  Zhang, Y., et al. 2023. Ann Hematol. 102: 3499-3513. PMID: 37713124
  9. USP43稳定c-Myc以促进膀胱癌的糖酵解和转移。  |  Li, M., et al. 2024. Cell Death Dis. 15: 44. PMID: 38218970
  10. 仅c-Myc就足以将成纤维细胞重编程为功能性巨噬细胞。  |  Li, S., et al. 2024. J Hematol Oncol. 17: 83. PMID: 39267119

订购信息

产品名称产品编号规格价格数量收藏夹

Myc 抗体 (C-33)

sc-42
200 µg/ml
RMB2377.00

Myc (C-33): m-IgG Fc BP-HRP 套装

sc-528091
200 µg Ab; 10 µg BP
RMB2662.00

Myc (C-33): m-IgGκ BP-HRP 套装

sc-520389
200 µg Ab, 40 µg BP
RMB2662.00

Myc (C-33): m-IgG1 BP-HRP 套装

sc-542755
200 µg Ab; 20 µg BP
RMB2662.00

Myc 抗体 (C-33) X

sc-42 X
200 µg/0.1 ml
RMB2377.00

Myc 抗体 (C-33) AC

sc-42 AC
500 µg/ml, 25% agarose
RMB3129.00

Myc 抗体 (C-33) HRP

sc-42 HRP
200 µg/ml
RMB2377.00

Myc 抗体 (C-33) FITC

sc-42 FITC
200 µg/ml
RMB2482.00

Myc 抗体 (C-33) PE

sc-42 PE
200 µg/ml
RMB2580.00

Myc 抗体 (C-33) Alexa Fluor® 488

sc-42 AF488
200 µg/ml
RMB2685.00

Myc 抗体 (C-33) Alexa Fluor® 546

sc-42 AF546
200 µg/ml
RMB2685.00

Myc 抗体 (C-33) Alexa Fluor® 594

sc-42 AF594
200 µg/ml
RMB2685.00

Myc 抗体 (C-33) Alexa Fluor® 647

sc-42 AF647
200 µg/ml
RMB2685.00

Myc 抗体 (C-33) Alexa Fluor® 680

sc-42 AF680
200 µg/ml
RMB2685.00

Myc 抗体 (C-33) Alexa Fluor® 790

sc-42 AF790
200 µg/ml
RMB2685.00

What is the difference between anti-Myc (9E10) SC-40 and (C-33) SC-42? I have ordered C-33 SC-42 HRP and used it to detect proteins fused with a Myc tag, but I was unable to detect any bands on the Western blot. Do you think it is worth buying SC-40?

Asked by: Abdo
Thank you for your question. The difference is the amino acid sequence and epitope. sc-42 was raised against the full length and sc-40 has an epitope range of 408-439. If you would like to trouble shoot the antibody we have a 1 year warranty from date of purchase . Please contact technical service by emailing [email protected] or call (800)-457-3801.
Answered by: BlakeJ
Date published: 2024-07-15

Could I get a quotation of 2mg of Myc Antibody (C-33): sc-42?

Asked by: Jinxiang
Thank you for your question. For accurate availability, shipping and pricing information regarding our antibodies, or to receive a quote, please contact Technical Service.
Answered by: Technical Service
Date published: 2024-05-23

What is the exact epitope for this antibody? I would need it to bind the sequence of chicken Myc and I am not sure if this antibody is suitable for that. Thanks in advance!

Asked by: Jen23
Thank you for your question. c-Myc (C-33) antibody was not epitope mapped. The antibody was raised against the full length. We would recommend this antibody for chicken there is a 38% homology.
Answered by: BlakeJ
Date published: 2024-03-23

What's the recommended concentration for use in FCM (Myc Antibody (C-33) FITC)?

Asked by: yamila7
Thank you for your question. We recommend 1 µg normal immunoglobulins per 1 million cells. https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: BlakeJ
Date published: 2024-03-23

Is this antibody suitable for detection of mouse c-Myc? I am planning to overexpress mouse c-Myc in mouse embryonic fibroblast cell line.

Asked by: IO11
Thank you for your question. c-Myc Antibody (C-33): sc-42 is recommended for detection of c-Myc p67 and c-Myc tagged fusion proteins of mouse, rat, human and avian origin by WB, IP, IF and ELISA.
Answered by: Tech Support Europe
Date published: 2024-06-03

Can this antibody detect endogenous c-Myc protein as well as fusion proteins tagged with c-Myc?

Asked by: AbPolly
Thank you for your question. Yes, our c-Myc sc-42 is reactive with both endogenous c-Myc in samples, and with c-Myc tagged proteins in multiple applications.
Answered by: Technical Support 12
Date published: 2024-03-23
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Rated 5 out of 5 by from Prodotto eccellenteBuon prodotto, lo comprerò seguramente in futuro.e
Date published: 2024-02-03
Rated 5 out of 5 by from 抗体用了好几家抗体，Santa一如既往的好。这个抗体效果太棒了，WB条带特别漂亮，价格也比较合理，推荐大家使用和购买
Date published: 2020-11-28
Rated 1 out of 5 by from 検出できません。HEK293T細胞に過剰発現したMycタグ付きのタンパク質を検出できませんでした。他社抗体では検出できます。
Date published: 2020-10-15
Rated 5 out of 5 by from Gives a clear band at around 55 kDaGood antibody. We use a 1:1000 dilution for the western blot.
Date published: 2019-03-21
Rated 5 out of 5 by from Great antibody for western blotThe batch of antibody I have worked well on BJAB, HeLa, Jurkat and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 500 times into 5% BSA, TBST and incubated over-night at 4°C.
Date published: 2018-01-04
Rated 1 out of 5 by from knockin mouse embryo IP2ug of Ab from sample failed to IP myc-tag protein. 9E10 and 9E11 worked fine.
Date published: 2017-12-27
Rated 2 out of 5 by from non specific bandsI see lots of non specific bands when I try to detect endogenous Myc in U251 cells(1:200) and do not know which is the right band.
Date published: 2017-11-21
Rated 5 out of 5 by from Works very wellThe antibody worked great for us under the following conditions: 5% milk, 1:500 dilution, HEK whole cell lysates with RIPA lysis buffer. It is recommended for the detection of endogenous c-Myc
Date published: 2017-10-05
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Myc 抗体 (C-33) is rated 4.4 out of 5 by 19.
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