Date published: 2025-9-8

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Brm 抗体 (E-1): sc-17828

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说明书
  • Brm 抗体 E-1 是小鼠单克隆 IgM κ,Brm抗体, 在12篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的Brm的N-terminus的氨基酸2-20之间
  • 推荐用于 mouse, rat, human 和 来源的Brm WB, IP, IF 和 ELISA检测; 也和以下物种反应,包括: equine and avian
  • 另提供琼脂糖偶联抗体,可用于IP实验
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-17828 X, 200 µg/0.1 ml)
  • 关于如何获取Brm (E-1): sc-17828免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成Brm 抗体 (E-1)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

Brm抗体(E-1)是一种IgM κ小鼠单克隆Brm抗体(也称为SWI/SNF相关基质相关肌动蛋白依赖的染色质亚家族A成员2抗体、HBRM抗体、BRM抗体、可能的全局转录激活剂SNF2L2抗体、ATP依赖的解旋酶SMARCA2抗体、BRG1相关因子190B抗体、Brahma同源抗体、SNF2-α抗体、BAF190B抗体、BAF190抗体、SNF2L2抗体、SNF2LA抗体、HSNF2a抗体、Sth1p抗体、SNF2抗体、SWI/SNF相关基质相关肌动蛋白依赖的染色质A2调节因子抗体或NF2/SWI2样蛋白2抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的Brm蛋白。Brm抗体(E-1)既可作为非偶联抗Brm抗体形式,也可作为多种偶联形式的抗Brm抗体,包括琼脂糖。Brahma蛋白(Brm)是果蝇Brm复合物的ATP酶亚基,与哺乳动物SWI/SNF染色质重塑复合物高度相关。Brm是Hox基因的转录激活剂,并与几乎所有转录活性染色质相关联,其模式与Polycomb不重叠,Polycomb是Hox基因转录的阻遏物。Brm复合物是三胸组蛋白Zeste必需的共激活因子,是同源基因的DNA结合激活剂。Brm功能的降低显著减少了RNA聚合酶II与果蝇唾液腺染色体的关联,这表明Brm复合物的染色质重塑活性在促进RNA聚合酶II转录中起着普遍作用。Brm通过抑制细胞周期蛋白E蛋白积累下游的S期进入,作为由果蝇细胞周期蛋白E的低效突变导致的粗糙眼表型的显性抑制因子。Brm复合物与染色质的相互作用可能受到BAP111的调节,BAP111通过HMG结构域与果蝇胚胎中的Brm复合物高度关联。Brm在未受精卵和早期胚胎中高表达。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

Brm 抗体 (E-1)

sc-17828
200 µg/ml
RMB2377.00

Brm 抗体 (E-1) AC

sc-17828 AC
500 µg/ml, 25% agarose
RMB3129.00

Brm (E-1) 中和勝肽

sc-17828 P
100 µg/0.5 ml
RMB511.00

Brm 抗体 (E-1) X

sc-17828 X
200 µg/0.1 ml
RMB2377.00

I am planning to do Immunofluorescence on mouse lung cancer tissues. Will this antibody work on them?

Asked by: Manendra
Thank you for your question. Brm (E-1) is recommend for mouse , rat and human samples for WB, IP, IF and ELISA.
Answered by: BlakeJ
Date published: 2024-11-22

Kindly let me know the compatible secondary for SC-17828 (BRM antibody). Will Anti-rabbit work with this. Also let me know the dilution for western blot

Asked by: priyabrata
Thank you for your question. Brm Antibody (E-1): sc-17828 is a mouse antibody, so an anti-mouse secondary antibody is necessary to detect it; an anti-rabbit antibody will not work. We recommend one of our anti-mouse binding proteins for secondary detection such as m-IgGκ BP-HRP: sc-516102 or m-IgGκ BP-CFL 790: sc-516181. According to this antibody's datasheet its recommended dilution for western blotting is 1:100 - 1:1000 with a starting dilution of 1:200.
Answered by: Tech Support Europe
Date published: 2020-07-01

What application is the blocking peptide sc-17828 P appropriate for?

Asked by: Dr Ninau Qelp
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Publishable EMSA and WB data with mouse MELPublishable EMSA and WB data with mouse MEL (murine erythroleukaemia) cells - SCBT Publication Review
Date published: 2015-04-21
Rated 5 out of 5 by from This antibody was used in a publication forThis antibody was used in a publication for IF on HEp-2 - SCBT Publication Review
Date published: 2015-03-01
Rated 5 out of 5 by from Successful application of this antibody inSuccessful application of this antibody in WB using hamster CHO-K1 -SCBT Publication Review
Date published: 2015-02-26
Rated 3 out of 5 by from Performs well in WesternPerforms well in Western, good signal strength in HeLa, NIH/3T3 and 3611-RF whole cell lysates. Some background detected -SCBT QC
Date published: 2015-01-06
Rated 5 out of 5 by from Clean blot with single strong band at expectedClean blot with single strong band at expected size using K-562 nuclear extract. -SCBT QC
Date published: 2015-01-05
Rated 5 out of 5 by from ChIP and WB publishable data with human MCF7ChIP and WB publishable data with human MCF7 cells - SCBT Publication Review
Date published: 2014-06-06
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Brm 抗体 (E-1) is rated 4.7 out of 5 by 6.
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