Date published: 2025-9-7
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brachyury Antibody (A-4) - Western Blotting - Image 398888
brachyury 抗体 (A-4) HRP: sc-374321 HRP. A549 (A), HeLa (B), Raji (C), NCI-H292 (D), Jurkat (E) 和 A-431 (F) 全细胞裂解液中 brachyury 表达的直接 Western 印迹分析.
brachyury 抗体 (A-4) HRP: sc-374321 HRP. A549 (A), HeLa (B), Raji (C), NCI-H292 (D), Jurkat (E) 和 A-431 (F) 全细胞裂解液中 brachyury 表达的直接 Western 印迹分析.

brachyury 抗体 (A-4): sc-374321

5.0(4)
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说明书
  • brachyury抗体(A-4)是小鼠单克隆IgG2b κ, 在18篇文献中引用，规格为200 µg/ml
  • 免疫human物种的brachyury的氨基酸226-43
  • 抗-brachyury 抗体 (A-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 brachyury
  • 抗brachyury抗体（A-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-374321 X, 200 µg/0.1 ml)
  • 关于如何获取brachyury (A-4): sc-374321的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 brachyury 抗体 (A-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与brachyury 抗体 (A-4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Brachyury抗体（A-4）是一种IgG2b κ小鼠单克隆Brachyury抗体，可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的Brachyury。Brachyury抗体（A-4）既可用作非偶联形式的抗Brachyury抗体，也可用作多种偶联形式的抗Brachyury抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。T-box基因家族由具有独特DNA结合域的成员组成。特征最明显的T-box（Tbx）基因，Brachyury或T，编码一种在早期脊椎动物发育中发挥重要作用的转录因子。Tbx基因是一个发育调控因子家族，最近在无脊椎动物和脊椎动物中发现了20多个成员。已发现Tbx基因的突变会导致多种人类疾病。对Tbx产物功能机制的理解主要来自于原型T/Brachyury蛋白，它是一种转录激活因子。T结构域是一个高度保守的DNA结合基序，最初在Brachyury中定义，是Tbx转录因子家族的特征。小鼠的Brachyury（T）基因是后中胚层形成和轴向发育所必需的。缺乏T基因功能的突变胚胎在脊索分化和后中胚层形成方面存在缺陷，但可以发育成前中胚层。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

brachyury 抗体 (A-4) 参考文献:

  1. Brachyury 是 Wnt/beta-catenin 信号通路的一个靶基因。  |  Arnold, SJ., et al. 2000. Mech Dev. 91: 249-58. PMID: 10704849
  2. Brachyury无突变体诱导的幼年海鞘内胚层器官缺陷。  |  Chiba, S., et al. 2009. Development. 136: 35-9. PMID: 19019990
  3. Brachyury和相关的Tbx蛋白与Mixl1同源结构域蛋白相互作用，并对Mixl1的转录活性进行负向调节。  |  Pereira, LA., et al. 2011. PLoS One. 6: e28394. PMID: 22164283
  4. 上皮-间质转化癌疫苗：癌细胞间质样表型的驱动因子Brachyury的组织分布。  |  Hamilton, DH., et al. 2012. Semin Oncol. 39: 358-66. PMID: 22595058
  5. Brachyury通过p27Kip1依赖性途径调节癌细胞的增殖。  |  Jezkova, J., et al. 2014. Oncotarget. 5: 3813-22. PMID: 25003467
  6. Brachyury在正常人类肠道隐窝和结肠直肠癌中识别一类肠内分泌细胞。  |  Jezkova, J., et al. 2016. Oncotarget. 7: 11478-86. PMID: 26862851
  7. Brachyury是疫苗靶标，在三阴性乳腺癌中过度表达。  |  Hamilton, DH., et al. 2016. Endocr Relat Cancer. 23: 783-796. PMID: 27580659
  8. Brachyury通过靶向SIRT1促进乳腺癌对他莫昔芬的耐药性。  |  Li, K., et al. 2016. Biomed Pharmacother. 84: 28-33. PMID: 27621036
  9. Brachyury癌基因是高危睾丸生殖细胞肿瘤的预后因素。  |  Pinto, F., et al. 2018. Andrology. 6: 597-604. PMID: 29749711
  10. 利用 CRISPR/CAS9 核酸酶生成 Brachyury-mCherry 基因敲入报告人多能干细胞系（SNUe003-A-2）。  |  Jung, J., et al. 2021. Stem Cell Res. 53: 102321. PMID: 33878708

订购信息

产品名称产品编号规格价格数量收藏夹

brachyury 抗体 (A-4)

sc-374321
200 µg/ml
RMB2377.00

brachyury (A-4): m-IgG Fc BP-HRP 套装

sc-529721
200 µg Ab; 10 µg BP
RMB2662.00

brachyury (A-4): m-IgGκ BP-HRP 套装

sc-522701
200 µg Ab, 40 µg BP
RMB2662.00

brachyury (A-4): m-IgG2b BP-HRP 套装

sc-550139
200 µg Ab; 10 µg BP
RMB2662.00

brachyury 抗体 (A-4) X

sc-374321 X
200 µg/0.1 ml
RMB2377.00

brachyury 抗体 (A-4) AC

sc-374321 AC
500 µg/ml, 25% agarose
RMB3129.00

brachyury 抗体 (A-4) HRP

sc-374321 HRP
200 µg/ml
RMB2377.00

brachyury 抗体 (A-4) FITC

sc-374321 FITC
200 µg/ml
RMB2482.00

brachyury 抗体 (A-4) PE

sc-374321 PE
200 µg/ml
RMB2580.00

brachyury 抗体 (A-4) Alexa Fluor® 488

sc-374321 AF488
200 µg/ml
RMB2685.00

brachyury 抗体 (A-4) Alexa Fluor® 546

sc-374321 AF546
200 µg/ml
RMB2685.00

brachyury 抗体 (A-4) Alexa Fluor® 594

sc-374321 AF594
200 µg/ml
RMB2685.00

brachyury 抗体 (A-4) Alexa Fluor® 647

sc-374321 AF647
200 µg/ml
RMB2685.00

brachyury 抗体 (A-4) Alexa Fluor® 680

sc-374321 AF680
200 µg/ml
RMB2685.00

brachyury 抗体 (A-4) Alexa Fluor® 790

sc-374321 AF790
200 µg/ml
RMB2685.00

Hi On IHC(P) tissue, how does your brachyury mab compare with your previous polyclonal antibody which seem not to be in your portofolio any more?

Asked by: hanh123
Thank you for your question. You can find IHC image data for this antibody on its product page and in the publication PMID 24223206, in which this and several other Santa Cruz antibodies have been used for IHC(P) applications.
Answered by: Tech Support Europe
Date published: 2019-08-28

I need to use an antibody to detect the protein in samples of equine origin. Do you know if sc-374321: brachyury (A-4) mouse monoclonal antibody will work?

Asked by: DefinitelyNotMatt
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2024-07-09
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Rated 5 out of 5 by from Good signal in IF on hiPSCsThe antibody was used in immunofluorescence (IF) on human iPS cells differentiated toward the mesoderm with an antibody dilution of 1:300. The staining in IF was good, we observed strong signal and with low background.
Date published: 2023-09-20
Rated 5 out of 5 by from Very good!We tried this antibody in whole-mount immunostaining of mouse embryos and liked it very much.
Date published: 2023-01-10
Rated 5 out of 5 by from Great for Western of Brachyury in mouse ES cellsGood antibody for Western of Brachyury(T) in mouse ES cells. Dilution 1:1000 in 5% Milk/TBST. Great results!
Date published: 2017-09-08
Rated 5 out of 5 by from Produced nice Western blot data of brachyuryProduced nice Western blot data of brachyury expression in A549 whole cell lysate. -SCBT QC
Date published: 2014-07-08
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brachyury 抗体 (A-4) is rated 5.0 out of 5 by 4.
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