Date published: 2025-9-8

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Bmi-1 抗体 (1F4): sc-13519

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说明书
  • Bmi-1抗体(1F4)是小鼠单克隆IgG2a κ, 在4篇文献中引用,规格为200 µg/ml
  • 针对Bmi-1融合蛋白的Drosophila melanogaster来源
  • 建议使用Bmi-1抗体(1F4)通过WB, IP和IF检测Bmi-1 of mouse and Drosophila origin, and PSC of Drosophila origin的mouse和Drosophila来源
  • 抗Bmi-1抗体(1F4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-13519 X, 200 µg/0.1 ml)
  • 关于如何获取Bmi-1 (1F4): sc-13519免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRP是Bmi-1 Antibody (1F4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Bmi-1 Antibody (1F4) 打包提供(请参阅下面的订购信息)。

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関連項目

Bmi-1抗体(1F4)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过应用诸如蛋白质印迹(WB)、免疫沉淀(IP)和免疫荧光(IF)等方法检测小鼠样品中的Bmi-1。Bmi-1 (1F4) 单克隆抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®结合物。Bmi-1在调节基因表达方面发挥着关键作用,是Polycomb组蛋白的关键成员,在胚胎发育过程中维持同源基因的抑制作用。Bmi-1主要位于细胞核中,与染色质相互作用,调节转录活性。Bmi-1的核定位对于基因沉默和细胞增殖功能至关重要,因为它直接影响染色质结构和靶基因的可及性。值得注意的是,Bmi-1与干细胞的自我更新和分化以及多种癌症的发生发展有关,因此成为肿瘤学和发育生物学研究的重要焦点。Bmi-1与其他蛋白质(如c-Myc和Pim)的相互作用进一步强调了其在致癌途径和淋巴瘤发生中的重要性,凸显了使用Bmi-1 (1F4)抗体等有效工具研究其在细胞过程中的不同作用的需求。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Bmi-1 抗体 (1F4)

sc-13519
200 µg/ml
RMB2377.00

Bmi-1 (1F4): m-IgG Fc BP-HRP 套装

sc-525473
200 µg Ab; 10 µg BP
RMB2662.00

Bmi-1 (1F4): m-IgG2a BP-HRP 套装

sc-546274
200 µg Ab; 10 µg BP
RMB2662.00

Bmi-1 抗体 (1F4) X

sc-13519 X
200 µg/0.1 ml
RMB2377.00

Bmi-1 抗体 (1F4) AC

sc-13519 AC
500 µg/ml, 25% agarose
RMB3129.00

Bmi-1 抗体 (1F4) HRP

sc-13519 HRP
200 µg/ml
RMB2377.00

Bmi-1 抗体 (1F4) FITC

sc-13519 FITC
200 µg/ml
RMB2482.00

Bmi-1 抗体 (1F4) PE

sc-13519 PE
200 µg/ml
RMB2580.00

Bmi-1 抗体 (1F4) Alexa Fluor® 488

sc-13519 AF488
200 µg/ml
RMB2685.00

Bmi-1 抗体 (1F4) Alexa Fluor® 546

sc-13519 AF546
200 µg/ml
RMB2685.00

Bmi-1 抗体 (1F4) Alexa Fluor® 594

sc-13519 AF594
200 µg/ml
RMB2685.00

Bmi-1 抗体 (1F4) Alexa Fluor® 647

sc-13519 AF647
200 µg/ml
RMB2685.00

Bmi-1 抗体 (1F4) Alexa Fluor® 680

sc-13519 AF680
200 µg/ml
RMB2685.00

Bmi-1 抗体 (1F4) Alexa Fluor® 790

sc-13519 AF790
200 µg/ml
RMB2685.00

When should the TransCruz reagent sc-13519 X be used instead of the standard form sc-13519?

Asked by: Trav11
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_9, d_7, h_4CST
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Rated 4 out of 5 by from Good antibodiesI get a trial package from company. I used this antibodies for WB in dilution 1:1000 in cell line. The detection was good.
Date published: 2018-06-10
Rated 4 out of 5 by from Good antibodiesI used this antibodies (dilution 1:1000) in detection BMI1 in cell lines and tissue sample. It works good.
Date published: 2018-04-29
Rated 1 out of 5 by from donot work in mouse ES cellsthis antibody do not work in mouse ES cells lysis.
Date published: 2017-03-20
Rated 5 out of 5 by from worked wellfirst try - worked well without much optimisation procedures neccessary
Date published: 2017-02-02
Rated 4 out of 5 by from Clean band in western blot analysis of DrosophilaClean band in western blot analysis of Drosophila PSC expression in 0-4 hour embryos and 12-24 hour embryos. Kindly provided by Paul Adler, University of Virginia. -SCBT QC
Date published: 2015-05-15
Rated 3 out of 5 by from Did not work in our ChIP experimentDid not work in our ChIP experiment- we are not sure if it is the antibody or our cell line.
Date published: 2015-03-19
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Bmi-1 抗体 (1F4) is rated 3.5 out of 5 by 6.
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