Date published: 2025-9-8

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beta Arrestin 1/2 抗体 (A-1): sc-74591

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说明书
  • beta Arrestin 1/2 抗体 A-1 是小鼠单克隆 IgG2a κ,beta Arrestin 1/2抗体, 在18篇文献中引用,规格为200 µg/ml
  • 免疫human来源β-Arrestin-1近N-terminus的7-290氨基酸
  • beta Arrestin 1/2 抗体 (A-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 β-Arrestin-1 and β-Arrestin-2; 也和以下物种反应,包括: porcine
  • 抗beta Arrestin 1/2抗体(A-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取beta Arrestin 1/2 (A-1): sc-74591免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 beta Arrestin 1/2 抗体 (A-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与beta Arrestin 1/2 抗体 (A-1) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品,请参阅我们的小鼠 IgG 结合蛋白完整列表。

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関連項目

β-Arrestin-1/2抗体(A-1)是一种小鼠单克隆IgG2a kappa轻链抗体,用于检测多种物种(包括小鼠、大鼠、人、牛和猪)的β-Arrestin-1和β-Arrestin-2。β-Arrestin-1/2 单克隆抗体(A-1)针对人类β-Arrestin-1蛋白N端附近的氨基酸7-290进行免疫,确保在应用中可靠检测,如蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)。 石蜡包埋切片(IHCP)和酶联免疫吸附测定(ELISA)等应用中实现可靠的检测。β-Arrestin-1和β-Arrestin-2是G蛋白偶联受体(GPCR)信号传导的关键调节因子,可调节受体的脱敏、内化,并启动独立于G蛋白的替代信号通路。从结构上看,这两种蛋白都由两个不同的结构域组成,即N端结构域和C端结构域,它们形成了一个双瓣结构,对于与磷酸化GPCR和各种信号蛋白的相互作用至关重要。翻译后修饰(如磷酸化、泛素化)通过调节β-Arrestin的相互作用、稳定性和细胞定位,在调节其功能方面发挥着重要作用。β-Arrestin-1和β-Arrestin-2与特定蛋白相互作用,包括G蛋白偶联受体激酶(GRKs)、clathrin和AP-2适配器复合物,这些蛋白对于受体内化和信号复合物的形成至关重要。这些相互作用对于维持细胞对激素、神经递质和感觉刺激的精确控制至关重要,从而影响激素信号传导、神经传递和免疫反应等过程。β-Arrestin-1和β-Arrestin-2主要在脾脏和神经组织中表达,对于维持细胞内稳态和促进对细胞外刺激的适当反应至关重要。建议研究β-Arrestin介导的信号传导通路结构和功能动态的研究人员使用抗-β-Arrestin-1/2抗体(A-1)。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

beta Arrestin 1/2 抗体 (A-1)

sc-74591
200 µg/ml
RMB2377.00

beta Arrestin 1/2 (A-1): m-IgGκ BP-HRP 套装

sc-521151
200 µg Ab, 40 µg BP
RMB2662.00

beta Arrestin 1/2 抗体 (A-1) AC

sc-74591 AC
500 µg/ml, 25% agarose
RMB3129.00

beta Arrestin 1/2 抗体 (A-1) HRP

sc-74591 HRP
200 µg/ml
RMB2377.00

beta Arrestin 1/2 抗体 (A-1) FITC

sc-74591 FITC
200 µg/ml
RMB2482.00

beta Arrestin 1/2 抗体 (A-1) PE

sc-74591 PE
200 µg/ml
RMB2580.00

beta Arrestin 1/2 抗体 (A-1) Alexa Fluor® 488

sc-74591 AF488
200 µg/ml
RMB2685.00

beta Arrestin 1/2 抗体 (A-1) Alexa Fluor® 546

sc-74591 AF546
200 µg/ml
RMB2685.00

beta Arrestin 1/2 抗体 (A-1) Alexa Fluor® 594

sc-74591 AF594
200 µg/ml
RMB2685.00

beta Arrestin 1/2 抗体 (A-1) Alexa Fluor® 647

sc-74591 AF647
200 µg/ml
RMB2685.00

beta Arrestin 1/2 抗体 (A-1) Alexa Fluor® 680

sc-74591 AF680
200 µg/ml
RMB2685.00

beta Arrestin 1/2 抗体 (A-1) Alexa Fluor® 790

sc-74591 AF790
200 µg/ml
RMB2685.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-25
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Rated 5 out of 5 by from KO検証しました正常細胞とbeta-arrestin1/2 KO細胞で検証したところ、正常細胞でしかバンドが出ませんでした。特異性が高い抗体だと思います
Date published: 2021-11-30
Rated 5 out of 5 by from This antibody was goodThis antibody was used to detect the ARRB1 protein and ARRB2 protein in ARRB1-overexpressed A549 cells and ARRB2-overexpressed A549 cells
Date published: 2019-12-04
Rated 5 out of 5 by from Endogenous and recombinant beta-arrestin1 in WB/IFProduct sc-74591 detected a unique band in non-transfected HEK293 cells and an additional band corresponding to transfected beta-arrestin1 recombinant proteins when used at 1:600 ratio followed by fluorescent secondary antibody detection IRDye 800CW used at 1:10,000 ratio. It did not detect recombinant beta-arrestin2 overexpressed in HEK293T cells. Immunocytochemistry gave a cytoplasmic staining with a slight nuclear staining when used at 1:200 ratio in HEK293T cells.
Date published: 2019-04-26
Rated 4 out of 5 by from good1/400 dilution was used for WB, specific band was shown.
Date published: 2018-12-24
Rated 5 out of 5 by from Great antibody!It is a very good antibody for both western blots and IP!
Date published: 2018-10-28
Rated 5 out of 5 by from Good for IFThis β-Arrestin-1/2 antibody shows strong specific signal in IF staining in primary rat motor neuron.
Date published: 2017-11-06
Rated 4 out of 5 by from β-Arrestin-1/2 Antibody westernWe have received a free sample of th bArresting 1/2 antibody. I am satisfied with the western blot, it worked the way it is shown in the picture.
Date published: 2017-07-03
Rated 3 out of 5 by from Try and install the responseWB stripe is not clear Difficult to raise,WB strips are less miscellaneous,
Date published: 2017-06-28
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beta Arrestin 1/2 抗体 (A-1) is rated 4.7 out of 5 by 16.
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