Date published: 2025-9-8

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Bcl-xL 抗体 (H-5): sc-8392

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说明书
  • Bcl-xL 抗体 H-5 是小鼠单克隆 IgG1 κ,Bcl-xL抗体, 在784篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的Bcl-xSL的C-terminus的氨基酸165-190之间
  • Bcl-xL 抗体 (H-5) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA,检测mouse, rat 和human 来源的 Bcl-xL; 不会与 Bcl-xS产生交联反应
  • Bcl-xL抗体(H-5)可与琼脂糖结合用于IP,与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 以及 Alexa Fluor® 405 用于IF, IHC(P) 和 FCM
  • 关于如何获取Bcl-xL (H-5): sc-8392免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 Bcl-xL 抗体 (H-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Bcl-xL 抗体 (H-5) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Bcl-xL抗体(H-5)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过多种应用检测小鼠、大鼠和人类来源的Bcl-xL,包括蛋白质印迹、免疫沉淀、免疫荧光、免疫组织化学、流式细胞术(FCM)和酶联免疫吸附测定。Bcl-xL单克隆抗体(H-5)有非结合型和多种结合型,如琼脂糖、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®结合物。Bcl-xL是Bcl-2家族的一员,在调节细胞凋亡方面发挥着关键作用,而细胞凋亡对于维持细胞内环境稳定和防止细胞过度死亡至关重要。Bcl-xL通过抑制细胞凋亡途径,有助于细胞在应对各种压力时存活,因此其在癌症治疗中尤为重要,因为Bcl-xL的过度表达会导致肿瘤形成和耐药性。Bcl-xL是一种233个氨基酸的蛋白质,与Bcl-2有43%的序列相似性,通过与促凋亡蛋白(如Bax和Bad)形成异二聚体来发挥作用,从而调节其活性并影响凋亡过程。了解Bcl-xL的相互作用和调节机制对于开发癌症治疗中的靶向疗法至关重要,因为操纵其表达或功能可以恢复癌细胞的凋亡反应。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Bcl-xL 抗体 (H-5) 参考文献:

  1. Bcl-xL 定时器设定了血小板的寿命。  |  Qi, B. and Hardwick, JM. 2007. Cell. 128: 1035-6. PMID: 17382877
  2. 在以细胞凋亡为特征的组织中,BCL2 蛋白的拓扑结构受到限制。  |  Hockenbery, DM., et al. 1991. Proc Natl Acad Sci U S A. 88: 6961-5. PMID: 1871110
  3. Bcl-2 基因表达失调可选择性地延长缺乏生长因子的造血细胞系的存活时间。  |  Nuñez, G., et al. 1990. J Immunol. 144: 3602-10. PMID: 2184193
  4. Bcl-xL 可作为 IP3R 通道的抑制剂,从而拮抗 Ca2+ 驱动的细胞凋亡。  |  Rosa, N., et al. 2022. Cell Death Differ. 29: 788-805. PMID: 34750538
  5. BCL-XL的过度表达可保护胰腺β细胞免受细胞因子和棕榈酸诱导的细胞凋亡。  |  Perez-Serna, AA., et al. 2023. Int J Mol Sci. 24: PMID: 36982731
  6. Bcl-xL通过CtBP2易位到细胞核,以表观遗传学的方式促进转移。  |  Zhang, T., et al. 2023. bioRxiv.. PMID: 37163116
  7. 鉴定小鼠 B 细胞系中免疫抑制剂诱导的细胞凋亡,以及 bcl-x 而非 bcl-2 对其的预防作用。  |  Gottschalk, AR., et al. 1994. Proc Natl Acad Sci U S A. 91: 7350-4. PMID: 7518929
  8. Bad是Bcl-XL和Bcl-2的异源二聚体伙伴,能取代Bax并促进细胞死亡。  |  Yang, E., et al. 1995. Cell. 80: 285-91. PMID: 7834748
  9. 用酵母双杂交系统分析 Bcl-2 蛋白家族成员之间的相互作用。  |  Sato, T., et al. 1994. Proc Natl Acad Sci U S A. 91: 9238-42. PMID: 7937747
  10. Bcl-2 的 BH1 和 BH2 结构域是抑制细胞凋亡和与 Bax 异源二聚化所必需的。  |  Yin, XM., et al. 1994. Nature. 369: 321-3. PMID: 8183370
  11. bcl-x,一种与 bcl-2 相关的基因,是细胞凋亡的主要调节因子。  |  Boise, LH., et al. 1993. Cell. 74: 597-608. PMID: 8358789
  12. Bcl-2 在体内与保守的同源物 Bax 异源二聚体,从而加速细胞的程序性死亡。  |  Oltvai, ZN., et al. 1993. Cell. 74: 609-19. PMID: 8358790

订购信息

产品名称产品编号规格价格数量收藏夹

Bcl-xL 抗体 (H-5)

sc-8392
200 µg/ml
RMB2377.00

Bcl-xL (H-5): m-IgG Fc BP-HRP 套装

sc-526515
200 µg Ab; 10 µg BP
RMB2662.00

Bcl-xL (H-5): m-IgG1 BP-HRP 套装

sc-531888
200 µg Ab; 20 µg BP
RMB2662.00

Bcl-xL 抗体 (H-5) AC

sc-8392 AC
500 µg/ml, 25% agarose
RMB3129.00

Bcl-xL 抗体 (H-5) FITC

sc-8392 FITC
200 µg/ml
RMB2482.00

Bcl-xL 抗体 (H-5) PE

sc-8392 PE
200 µg/ml
RMB2580.00

Bcl-xL 抗体 (H-5) Alexa Fluor® 488

sc-8392 AF488
200 µg/ml
RMB2685.00

Bcl-xL 抗体 (H-5) Alexa Fluor® 546

sc-8392 AF546
200 µg/ml
RMB2685.00

Bcl-xL 抗体 (H-5) Alexa Fluor® 594

sc-8392 AF594
200 µg/ml
RMB2685.00

Bcl-xL 抗体 (H-5) Alexa Fluor® 647

sc-8392 AF647
200 µg/ml
RMB2685.00

Bcl-xL 抗体 (H-5) Alexa Fluor® 680

sc-8392 AF680
200 µg/ml
RMB2685.00

Bcl-xL 抗体 (H-5) Alexa Fluor® 790

sc-8392 AF790
200 µg/ml
RMB2685.00

Bcl-xL 抗体 (H-5) TRITC

sc-8392 TRITC
200 µg/ml
RMB2407.00

Bcl-xL 抗体 (H-5) Alexa Fluor® 405

sc-8392 AF405
200 µg/ml
RMB2685.00

Bcl-xL (H-5) 阻断肽

sc-8392 P
100 µg/0.5 ml
RMB511.00

I would like to try and use the Bcl-xL Antibody (H-5) for detection in bovine tissue samples. Will it work?

Asked by: Trav11
If you would like to use the Bcl-xL Antibody (H-5): sc-8392 for a species that is not listed under recommended species, please contact our Technical Service department at 800-457-3801 and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Tech Service 8
Date published: 2016-12-16

How should I go about using this antibody for double or triple staining when my other primary antibodies were also raised in mice?

Asked by: Trav11
In this case, we recommend using our directly conjugated mouse monoclonal primary antibodies. The Bcl-xL Antibody (H-5): sc-8392 is currently available conjugated to HRP (sc-8392 HRP), phycoerythrin (sc-8392 PE), fluorescein (sc-8392 FITC), Alexa Fluor® 488 (sc-8392 AF488), Alexa Fluor® 647 (sc-8392 AF647), TRITC (sc-8392 TRITC, 200 µg/ml), PerCP (sc-8392 PerCP) or Alexa Fluor® 405 (sc-8392 AF405).
Answered by: Tech Service 8
Date published: 2016-12-16
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Rated 4 out of 5 by from Great for WBThis primary antibody is useful in western blotting.
Date published: 2024-03-21
Rated 5 out of 5 by from western blotThe antibody can gave a nice clean band. It is a good antibody.
Date published: 2023-03-10
Rated 5 out of 5 by from Bcl-xL Ab in western blotVery good Ab used in human neutrophils (dilution 1/200 in TBST milk5%)
Date published: 2018-03-13
Rated 3 out of 5 by from Weak signal but MW correspondsTested in purified mouse brain mitochondria and there was a band corresponding to the expected MW, although weak
Date published: 2018-01-17
Rated 5 out of 5 by from Great antibodyIt work perfectly WB detection in HCT116 cell lines. Strong and clear band.
Date published: 2017-11-29
Rated 3 out of 5 by from 抗体一般该试用装抗体的使用效果一般,条带有点模糊,背景也不是很好,希望公司继续努力,尽量改进该抗体的质量,谢谢。
Date published: 2017-09-08
Rated 5 out of 5 by from Great features!I got free sample several months ago, and I used the recommanded dilution rate ,and the western blot was nice, but I didn't use it in immunohistochemical! Thank you!
Date published: 2017-09-04
Rated 4 out of 5 by from 还不错一周前拿到此产品,在293T细胞上进行内源性检测,Western Blot结果显示条带大小正确,比较清晰
Date published: 2017-09-01
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Bcl-x L 抗体 (H-5) is rated 4.4 out of 5 by 37.
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