Date published: 2025-9-7
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Bax Antibody (2D2) - Western Blotting - Image 390868
Bax 抗体 (2D2) Alexa Fluor® 647: sc-20067 AF647. 直接荧光西部印迹分析 MCF7 (A), SW480 (B), HT-1080 (C), HeLa (D), Ramos (E) 和 BJAB (F) 全细胞裂解液中 Bax 的表达. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
Bax 抗体 (2D2) Alexa Fluor® 647: sc-20067 AF647. 直接荧光西部印迹分析 MCF7 (A), SW480 (B), HT-1080 (C), HeLa (D), Ramos (E) 和 BJAB (F) 全细胞裂解液中 Bax 的表达. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

Bax 抗体 (2D2): sc-20067

4.9(29)
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说明书
  • Bax抗体(2D2)是小鼠单克隆IgG1 κ, 在505篇文献中引用，规格为200 µg/ml
  • 与human的Bax的氨基酸3-16对应的合成肽杂交
  • 抗-Bax 抗体 (2D2) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 Bax
  • 抗Bax抗体（2D2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Bax (2D2): sc-20067的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Bax 抗体 (2D2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Bax 抗体 (2D2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Bax 抗体包括 Bax β

Bax抗体（2D2）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和流式细胞术（FCM）等方法检测小鼠、大鼠和人类样本中的Bax。Bax (2D2) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物，可满足不同实验需求。Bax 在细胞凋亡调节中起着关键作用，是 Bcl-2 蛋白家族中促凋亡的成员。Bax在响应各种应激信号时促进细胞死亡，从而维持细胞内环境平衡并防止不受控制的细胞增殖，这在癌症中尤为重要。Bax主要定位于线粒体外膜，在那里Bax促进细胞色素c和其他促凋亡因子从线粒体中释放，从而触发凋亡级联反应。Bax可以与Bcl-2形成异二聚体，抵消Bcl-2的抗凋亡作用，从而影响细胞命运的决定。了解Bax的动态和相互作用对于制定治疗策略以调节癌症等疾病中的细胞凋亡至关重要，因为癌症中细胞存活和死亡之间的平衡经常被打破。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Bax 抗体 (2D2)

sc-20067
200 µg/ml
$316.00

Bax (2D2): m-IgG Fc BP-HRP 套装

sc-526598
200 µg Ab; 10 µg BP
$354.00

Bax (2D2): m-IgG1 BP-HRP 套装

sc-531971
200 µg Ab; 20 µg BP
$354.00

Bax 抗体 (2D2) AC

sc-20067 AC
500 µg/ml, 25% agarose
$416.00

Bax 抗体 (2D2) HRP

sc-20067 HRP
200 µg/ml
$316.00

Bax 抗体 (2D2) FITC

sc-20067 FITC
200 µg/ml
$330.00

Bax 抗体 (2D2) PE

sc-20067 PE
200 µg/ml
$343.00

Bax 抗体 (2D2) Alexa Fluor® 488

sc-20067 AF488
200 µg/ml
$357.00

Bax 抗体 (2D2) Alexa Fluor® 546

sc-20067 AF546
200 µg/ml
$357.00

Bax 抗体 (2D2) Alexa Fluor® 594

sc-20067 AF594
200 µg/ml
$357.00

Bax 抗体 (2D2) Alexa Fluor® 647

sc-20067 AF647
200 µg/ml
$357.00

Bax 抗体 (2D2) Alexa Fluor® 680

sc-20067 AF680
200 µg/ml
$357.00

Bax 抗体 (2D2) Alexa Fluor® 790

sc-20067 AF790
200 µg/ml
$357.00

why am i not getting any band in IP of Bax. I have tried for 4 times.

Asked by: celesta
Thank you for your message. Please contact our technical service team directly for troubleshooting advice on your experiment.
Answered by: Tech Support Europe
Date published: 2023-09-18

I am going to be performing a double stain on my samples. How can I do this with only mouse monoclonal antibodies?

Asked by: Cweed
We recommend using directly conjugated primary antibodies. This product, sc-20067, is available directly conjugated to AF488, AF647, and FITC.
Answered by: TechService7
Date published: 2017-01-04
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Rated 5 out of 5 by from Excellent product for flow cytometryI used this antibody for flow cytometry studies with different human cell lines and it worked perfectly all the times. I would recommend this antibody!
Date published: 2023-04-05
Rated 5 out of 5 by from western blotThe antibody can gave a nice clean band. It is a good antibody.
Date published: 2023-03-10
Rated 5 out of 5 by from Excellent results in WBVery strong band in human cancer primary cultures (1:1000 dilution)
Date published: 2019-03-02
Rated 5 out of 5 by from Work wellIt is working at the dilution of 1:500,the band is quite clear
Date published: 2018-04-04
Rated 5 out of 5 by from Quite good antibody!1:500 diluted for wb of rat protein ,the band is quite strong and special!
Date published: 2017-12-30
Rated 3 out of 5 by from AverageI get it over a month ago, but I did not get a good band for bax.
Date published: 2017-10-30
Rated 5 out of 5 by from Bax antibodyWe've been using anti-Bax-PE for several years in flow cytometry studies and it has been working very well.
Date published: 2017-10-27
Rated 5 out of 5 by from Great antibodyDetect a single band from a hASMC extract on Western blot!
Date published: 2017-10-18
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Bax 抗体 (2D2) is rated 4.9 out of 5 by 29.
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