Date published: 2025-9-7
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Bax Antibody (B-9) - Western Blotting - Image 389997
Bax 抗体 (B-9) Alexa Fluor® 790: sc-7480 AF790. 对 Ramos (A), CCRF-CEM (B), COLO 320DM (C) 和 RAW 264. 7 (D) 全细胞裂解液中 Bax 的表达进行直接近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
Bax 抗体 (B-9) Alexa Fluor® 790: sc-7480 AF790. 对 Ramos (A), CCRF-CEM (B), COLO 320DM (C) 和 RAW 264. 7 (D) 全细胞裂解液中 Bax 的表达进行直接近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

Bax 抗体 (B-9): sc-7480

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说明书
  • Bax 抗体 B-9 是小鼠单克隆 IgG2b κ，Bax抗体, 在1,528篇文献中引用，规格为200 µg/ml
  • 免疫mouse物种的Baxα的氨基酸1-171
  • Bax 抗体 (B-9) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 Baxα and Baxβ
  • Bax抗体（B-9）可与琼脂糖结合用于IP，与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 以及 Alexa Fluor® 405 用于IF, IHC(P) 和 FCM
  • 关于如何获取Bax (B-9): sc-7480的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是Bax Antibody (B-9) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Bax Antibody (B-9) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Bax 抗体包括 Bax β

Bax抗体（B-9）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹、免疫沉淀、免疫荧光、免疫组织化学和流式细胞术等方法检测小鼠、大鼠和人源Bax蛋白。Bax (B-9) 抗体有非结合型和多种结合型，包括琼脂糖、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合型。Bax 蛋白在细胞凋亡调节中起着关键作用，是 Bcl-2 蛋白家族中促凋亡的成员。Bax通过促进细胞色素c从线粒体中释放，促进细胞凋亡酶的激活，而细胞凋亡酶对于执行程序性细胞死亡至关重要。这一功能对于维持细胞稳态和防止细胞无序增殖（可能导致癌症）尤为重要。值得注意的是，Bax可以与Bcl-2（一种广为人知的抗凋亡蛋白）形成同源二聚体和异源二聚体，从而影响细胞存活和死亡之间的平衡。Bax (B-9)抗体的特异性可确保检测Bax，而不会与Bcl-2产生交叉反应，因此该试剂是研究细胞凋亡途径的重要工具。实验表明，Bax的过度表达会加速细胞因细胞因子缺失而凋亡，这凸显了其在细胞应激反应和生长因子缺失中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Bax 抗体 (B-9) 参考文献:

  1. 醛糖还原酶介导内毒素诱导小鼠巨噬细胞产生一氧化氮和细胞毒性。  |  Ramana, KV., et al. 2007. Free Radic Biol Med. 42: 1290-302. PMID: 17382209
  2. M11L的结构：一种凋亡抑制因子Bcl-2的肌瘤病毒结构同源物。  |  Douglas, AE., et al. 2007. Protein Sci. 16: 695-703. PMID: 17384234
  3. 口腔鳞状细胞癌原发肿瘤和淋巴节面转移瘤中 Bcl-xL, Bax 和 p53 的表达。  |  Baltaziak, M., et al. 2006. Ann N Y Acad Sci. 1090: 18-25. PMID: 17384243
  4. 用抗肿瘤药物依托泊苷处理人类白血病细胞株 HL60 后，凋亡相关基因 BCL2, BAX, FAS, caspase-3 和新成员 BCL2L12 的 mRNA 表达发生变化。  |  Floros, KV., et al. 2006. Ann N Y Acad Sci. 1090: 89-97. PMID: 17384250
  5. 在以细胞凋亡为特征的组织中，BCL2 蛋白的拓扑结构受到限制。  |  Hockenbery, DM., et al. 1991. Proc Natl Acad Sci U S A. 88: 6961-5. PMID: 1871110
  6. Bcl-2 基因表达失调可选择性地延长缺乏生长因子的造血细胞系的存活时间。  |  Nuñez, G., et al. 1990. J Immunol. 144: 3602-10. PMID: 2184193
  7. bcl-2 候选原癌基因产物是一种 24 千道尔顿的完整膜蛋白，在携带 t（14;18）易位的淋巴细胞系和淋巴瘤中高度表达。  |  Chen-Levy, Z., et al. 1989. Mol Cell Biol. 9: 701-10. PMID: 2651903
  8. Bcl-2 基因促进造血细胞存活，并与 c-myc 合作使前 B 细胞永生。  |  Vaux, DL., et al. 1988. Nature. 335: 440-2. PMID: 3262202
  9. BAX的生理学和药理学调节。  |  Spitz, AZ. and Gavathiotis, E. 2022. Trends Pharmacol Sci. 43: 206-220. PMID: 34848097
  10. 小鼠视网膜神经节细胞中的 BAX 激活分为两个时间和机制上截然不同的步骤。  |  Maes, ME., et al. 2023. Mol Neurodegener. 18: 67. PMID: 37752598
  11. 克隆 t(14;18)人类淋巴瘤的染色体断点：聚集在 14 号染色体上的 JH 周围和 18 号染色体上的一个转录单元附近。  |  Bakhshi, A., et al. 1985. Cell. 41: 899-906. PMID: 3924412
  12. Bcl-2 在体内与保守的同源物 Bax 异源二聚体，从而加速细胞的程序性死亡。  |  Oltvai, ZN., et al. 1993. Cell. 74: 609-19. PMID: 8358790

订购信息

产品名称产品编号规格价格数量收藏夹

Bax 抗体 (B-9)

sc-7480
200 µg/ml
RMB2377.00

Bax (B-9): m-IgGκ BP-HRP 套装

sc-520502
200 µg Ab, 40 µg BP
RMB2662.00

Bax (B-9): m-IgG2b BP-HRP 套装

sc-548833
200 µg Ab; 10 µg BP
RMB2662.00

Bax 抗体 (B-9) AC

sc-7480 AC
500 µg/ml, 25% agarose
RMB3129.00

Bax 抗体 (B-9) FITC

sc-7480 FITC
200 µg/ml
RMB2482.00

Bax 抗体 (B-9) PE

sc-7480 PE
200 µg/ml
RMB2580.00

Bax 抗体 (B-9) Alexa Fluor® 488

sc-7480 AF488
200 µg/ml
RMB2685.00

Bax 抗体 (B-9) Alexa Fluor® 546

sc-7480 AF546
200 µg/ml
RMB2685.00

Bax 抗体 (B-9) Alexa Fluor® 594

sc-7480 AF594
200 µg/ml
RMB2685.00

Bax 抗体 (B-9) Alexa Fluor® 647

sc-7480 AF647
200 µg/ml
RMB2685.00

Bax 抗体 (B-9) Alexa Fluor® 680

sc-7480 AF680
200 µg/ml
RMB2685.00

Bax 抗体 (B-9) Alexa Fluor® 790

sc-7480 AF790
200 µg/ml
RMB2685.00

Bax 抗体 (B-9) TRITC

sc-7480 TRITC
200 µg/ml
RMB2407.00

Bax 抗体 (B-9) Alexa Fluor® 405

sc-7480 AF405
200 µg/ml
RMB2685.00

For flowcytometry can you share some articles proving BAX antibody and its isotypic control use? Also, can you please share of Flowcytometry staining protocol for this antibody and its isotypic control?

Asked by: RAMYAR
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Hi. May I know if your antibody is recombinant or hybridoma?

Asked by: Nghia
For more information on our Monoclonal Antibodies please contact Technical Service.
Answered by: Tech Sales
Date published: 2021-05-12

 I run my sample on 10 % gel, I used 1/1000 in 5% BSA in TBS with  3h incubation, and I didn’t get any bands. Would you please advice me. 1)  What is the recommended dilution? The  dilute should be milk or BSA?  2) How long should I Incubate?What gel %?

Asked by: ddjoseph
Thank you for your question. We would recommend using the starting dilution for Western blot (1:200) and blocking your membrane with, and incubating the antibodies in 5% nonfat milk in TBST. For this protein we recommend a 12% gel. A link to our recommended protocol is below. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2020-04-21

is there is a gift samples is available for BAX Bcl2?

Asked by: Dhana
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2020-03-10

I have three questions: 1) What gel percentage do you use to separate Baxα and Baxβ? 2) Does Bax Antibody (B-9): sc-7480 works for IF like the (6A7) clone: sc-23959? 3) If so, in human, in mouse cells or both species' cell lines?

Asked by: tiosulphat3
Thank you for your question. We are running a 15% SDS-PAGE gel for detection of Bax with this antibody. Both antibodies are recommended for IF on mouse, rat and human samples.
Answered by: Technical Support
Date published: 2020-03-10

Could you please suggest a suitable secondary antibody kit for this product as I am going to perform IHC (P) in Rat testis?

Asked by: Senthil
Thank you for your question. You can find our secondary reagents here: https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4
Answered by: Technical Support
Date published: 2020-03-10

can i use Bax Antibody (B-9) in goat? if yes what procedure do i follow?

Asked by: yana79
Thank you for the question. We recommended Bax Antibody (B-9) for detection of Baxα and Baxβ of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. Unfortunately, we have not evaluated this antibody on samples derived from other animal species. Therefore, we are unable to predict cross-reactivity with the Bax protein from goat. If you were to try this, you might want to check and follow our protocols: https://www.scbt.com/scbt/resources/protocols
Answered by: Technical Support Europe
Date published: 2020-03-10

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 3
Date published: 2020-03-10
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Rated 1 out of 5 by from This antibody is not specific to BaxThis antibody show false positive detection of proteins at the expected molecular weight between 20 and 25 kDa. This is shown by knock out studies and comparison with other anti-Bax antibodies. Please see a recent article by Entrop, K. et al., 2024, Cell Death & Disease: https://www.nature.com/articles/s41419-024-07273-6 I urge SCBT to stop offering this antibody until it has been verified by knock out studies that the antibody is specific. This information should be made publicly available.
Date published: 2025-01-05
Rated 4 out of 5 by from Great for WBIt is a good antibody in WB. It works in dilution 1:1000.
Date published: 2024-04-01
Rated 5 out of 5 by from GreatI use this antobody for Western Blot. I get a positive result. I would recommend this great antibody to my colleagues.
Date published: 2023-11-20
Rated 4 out of 5 by from Good for WBWork well for Western blot (WB) in rat renal tubular cells
Date published: 2023-08-18
Rated 5 out of 5 by from Bax antibodyThe antibody is very good on western blot. The band is clear .
Date published: 2023-08-06
Rated 5 out of 5 by from It works well!This antibody works well at 1:500 dilution, and no other bands were detected with high specificity.
Date published: 2021-09-17
Rated 5 out of 5 by from Ottimo funzionamentoAnticorpo acquistato un anno fa e conservato correttamente a 4°C. Testato tramite Western Blotting su cellule provenienti da topi CD1. Ottima presenza della banda specifica.
Date published: 2021-05-28
Rated 5 out of 5 by from Great antibody for WBThis primary antibody worked very well in Western blot.
Date published: 2021-03-31
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Bax 抗体 (B-9) is rated 4.6 out of 5 by 86.
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