Date published: 2025-9-8
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BAP1 Antibody (C-4) - Western Blotting - Image 380967
BAP1 抗体 (C-4) HRP: sc-28383 HRP. KNRK (A), PC-3 (B), A-431 (C), MCF7 (D), Jurkat (E) 和 THP-1 (F) 全细胞裂解液中 BAP1 表达的直接 Western 印迹分析.
BAP1 抗体 (C-4) HRP: sc-28383 HRP. KNRK (A), PC-3 (B), A-431 (C), MCF7 (D), Jurkat (E) 和 THP-1 (F) 全细胞裂解液中 BAP1 表达的直接 Western 印迹分析.

BAP1 抗体 (C-4): sc-28383

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寫評論提問

说明书
  • BAP1 抗体 C-4 是小鼠单克隆 IgG1 κ，BAP1抗体, 在337篇文献中引用，规格为200 µg/ml
  • 抗-BAP1 抗体 (C-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 BAP 1
  • BAP1抗体（C-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取BAP1 (C-4): sc-28383的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 BAP1 抗体 (C-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与BAP1 抗体 (C-4) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

BAP1抗体（C-4）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的BAP1蛋白。BAP1抗体（C-4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。BAP1，又称BRCA1相关蛋白1，是一种泛素水解酶，对调节蛋白质降解和维持细胞内环境平衡至关重要。抗BAP1抗体（C-4）可增强BRCA1介导的细胞生长抑制作用，有助于预防癌症。位于17q染色体上的BRCA1基因突变与约45%的早发性乳腺癌和卵巢癌高发家庭有关。BAP1由729个氨基酸组成，并与其他蛋白质相互作用，例如BARD1，后者在肿瘤抑制中也起着重要作用。BAP1 (C-4)抗体与BRCA1结合并参与关键的细胞通路，这使得该产品对于癌症研究和治疗开发具有价值。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

BAP1 抗体 (C-4) 参考文献:

  1. 位于染色体 17q12-q23 上的家族性乳腺癌-卵巢癌基因座。  |  Narod, SA., et al. 1991. Lancet. 338: 82-3. PMID: 1676470
  2. 早发家族性乳腺癌与染色体 17q21 的联系。  |  Hall, JM., et al. 1990. Science. 250: 1684-9. PMID: 2270482
  3. BAP1的药剂抑制作用可招募HERC2，从而竞争性地分离BRCA1-BARD1，抑制DNA修复，并使CRC对放射治疗敏感。  |  Yue, X., et al. 2023. Acta Pharm Sin B. 13: 3382-3399. PMID: 37655321
  4. 超越BAP1失活黑色素瘤的典型组织学。  |  Donati, M. and Kazakov, DV. 2024. Pathol Res Pract. 259: 155162. PMID: 38326181
  5. ATF2/BAP1 轴通过 P53 通路介导蛛网膜下腔出血后的神经元凋亡  |  Tian, Q., et al. 2024. Stroke. 55: 2113-2125. PMID: 38965653
  6. 肿瘤抑制因子BAP1通过调节SLC7A11和NADPH水平抑制二硫键断裂。  |  Wang, J., et al. 2024. Oncogenesis. 13: 31. PMID: 39266549
  7. 原发性乳腺癌和卵巢癌中的 BRCA1 基因突变。  |  Futreal, PA., et al. 1994. Science. 266: 120-2. PMID: 7939630
  8. 乳腺癌基因带来惊喜  |  Nowak, R. 1994. Science. 265: 1796-9. PMID: 8091205
  9. 鉴定可在体内与 BRCA1 基因产物相互作用的 RING 蛋白。  |  Wu, LC., et al. 1996. Nat Genet. 14: 430-40. PMID: 8944023
  10. 细胞周期依赖于 BARD1 和 BRCA1 蛋白在离散核域中的共定位。  |  Jin, Y., et al. 1997. Proc Natl Acad Sci U S A. 94: 12075-80. PMID: 9342365
  11. 原发性乳腺癌, 卵巢癌和子宫癌中 BRCA1 相关 RING 结构域 (BARD1) 基因的突变。  |  Thai, TH., et al. 1998. Hum Mol Genet. 7: 195-202. PMID: 9425226
  12. BAP1：一种新型泛素水解酶，可与 BRCA1 RING 手指结合并增强 BRCA1 介导的细胞生长抑制作用。  |  Jensen, DE., et al. 1998. Oncogene. 16: 1097-112. PMID: 9528852

订购信息

产品名称产品编号规格价格数量收藏夹

BAP1 抗体 (C-4)

sc-28383
200 µg/ml
$316.00

BAP1 (C-4): m-IgG Fc BP-HRP 套装

sc-528441
200 µg Ab; 10 µg BP
$354.00

BAP1 (C-4): m-IgGκ BP-HRP 套装

sc-520819
200 µg Ab, 40 µg BP
$354.00

BAP1 (C-4): m-IgG1 BP-HRP 套装

sc-542933
200 µg Ab; 20 µg BP
$354.00

BAP1 抗体 (C-4) AC

sc-28383 AC
500 µg/ml, 25% agarose
$416.00

BAP1 抗体 (C-4) HRP

sc-28383 HRP
200 µg/ml
$316.00

BAP1 抗体 (C-4) FITC

sc-28383 FITC
200 µg/ml
$330.00

BAP1 抗体 (C-4) PE

sc-28383 PE
200 µg/ml
$343.00

BAP1 抗体 (C-4) Alexa Fluor® 488

sc-28383 AF488
200 µg/ml
$357.00

BAP1 抗体 (C-4) Alexa Fluor® 546

sc-28383 AF546
200 µg/ml
$357.00

BAP1 抗体 (C-4) Alexa Fluor® 594

sc-28383 AF594
200 µg/ml
$357.00

BAP1 抗体 (C-4) Alexa Fluor® 647

sc-28383 AF647
200 µg/ml
$357.00

BAP1 抗体 (C-4) Alexa Fluor® 680

sc-28383 AF680
200 µg/ml
$357.00

BAP1 抗体 (C-4) Alexa Fluor® 790

sc-28383 AF790
200 µg/ml
$357.00

BAP1- clone C-4 comes with predilute.

Asked by: Ronald Advincula
Thank you for your question. The antibody does not come pre-diluted .
Answered by: BlakeJ
Date published: 2024-04-02

Hei, I need SDS-Sheet for BAP1, C4, SC-28383

Asked by: SSHF KRS
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2024-04-01

Which BAP-1 antibody would be best for IHC-staining in uveal melanoma (human tissue)? Is there a protocol we could use?

Asked by: KHoesel
Thank you for your question. BAP1 Antibody (C-4): sc-28383 is the only antibody we currently offer that is recommended for both IHC applications and human tissues and cells. You can find our recommended protocol for IHC staining by following this link: https://www.scbt.com/scbt/de/resources/protocols/immunoperoxidase-staining Feel free to also contact our technical service team anytime for further assistance.
Answered by: Tech Support Europe
Date published: 2020-06-25

Do you know if this antibody will work for zebrafish?

Asked by: abc123
Thank you for your question. This antibody has not yet been tested in zebrafish and so that is not a recommended species at this time. We are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2020-01-02

What codons of the BAP1 protein is this antibody detecting?

Asked by: Kim Monkhorst
Thank you for your question. The epitope for this antibody maps within amino acids 430-729 of BAP 1 of human origin (accession#Q92560). The exact epitope has not been mapped.
Answered by: Tech Service
Date published: 2023-11-27

What is a good control tissue to use for BAP1  immunohistochemistry optimisation?

Asked by: GWAT1
Thank you for your question. Any tissue that expressed BAP1 would be a good control. We suggest duodenum or testis tissue as suitable controls.
Answered by: Tech Service
Date published: 2019-06-03

Hi, I'm having difficulty optimizing lot H0417. I are experiencing excessive background staining. (We ran our on Ultra Benchmark, CC1 and 64minutue, option 4 at 16 minute, Antibody incubation time is 1 hr, blocker). Please advise.

Asked by: IHC Tech
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-01-26

Dear, Is it possible to receive a free sample of the antibody BAP-1 (clone C4) refn° sc-28383

Asked by: doris peters
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-14
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Rated 2 out of 5 by from Inconsistent staining results of BAP1 AntibodyThis antibody produces high background signal on immunostaining. Although I tried to optimize the conditions, the background was still high. Although the efficiency of antibody varies among different batches. I tried 3 different batches; only one worked but produced high background, while the other two batches did not work at all in if, despite using the same conditions.
Date published: 2025-07-06
Rated 5 out of 5 by from works wellworks well for WB - used human cell lysates (HEK293T cells)
Date published: 2022-12-27
Rated 1 out of 5 by from 抗体染不上色！我买了这个BAP1 C4抗体，然而免疫组化做了好几次都染不上色，已经用了最 大浓度了，技术支持也很差
Date published: 2021-12-18
Rated 2 out of 5 by from 中皮腫症例を染色したものの…Leica BOND Maxで50倍希釈、PAb15分、pH6,pH9のそれぞれで20分賦活しました。結果は芳しくなく、あまりpotentialを感じませんでした。
Date published: 2021-09-28
Rated 5 out of 5 by from Works well for WesternUsed in 1:500 dilution to detect BAP1 in HEK293 whole cell extracts
Date published: 2019-02-01
Rated 1 out of 5 by from Inconstant staining resultsWith one batch (test sample), we obtained plausible staining results (Ventana; CC1/32min, 8' amplification, dilution 1:75/32min; nuclear loss in the tumor cells with mutation; nuclear expression in vascular endothelia and leukocytes as well as in tumors without mutation). With another batch (regularly ordered) identical staining procedures revealed strong background staining, which remained even at much higher dilutions of the antibody even in a mutated case.
Date published: 2018-02-27
Rated 4 out of 5 by from BAP1 trialI optimised this antibody for diagnosis of BAPoma on skin biopsy. I achieved good staining results using the trial sample and the lab will be adding this antibody to our repertoire in the near future.
Date published: 2017-11-24
Rated 5 out of 5 by from good antibodyI got this antibody as an sample to test it in prostate cancer cell line and found the antibody is good for WB 1:2000 dilution.
Date published: 2017-09-04
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BAP1 抗体 (C-4) is rated 4.1 out of 5 by 17.
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