BACE HDR Plasmid (h): sc-401500-HDR. Western blot analysis of BACE expression in non-transfected control(A) and puromycin treated, BACE CRISPR/Cas9 KO Plasmid: sc-401500 plus BACE HDR Plasmid co-transfected (B) HEK 293T whole cell lysates. Blot probed with BACE (A-12): sc-365948. β-Actin (C4): sc-47778 used as specificity and loading control.
BACE HDR Plasmid (h): sc-401500-HDR. Western blot analysis of BACE expression in non-transfected control(A) and puromycin treated, BACE CRISPR/Cas9 KO Plasmid: sc-401500 plus BACE HDR Plasmid co-transfected (B) HEK 293T whole cell lysates. Blot probed with BACE (A-12): sc-365948. β-Actin (C4): sc-47778 used as specificity and loading control.