Date published: 2025-9-8
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B7-H3 Antibody (F-11) - Western Blotting - Image 159000
B7-H3 抗体 (F-11): sc-376769. 在 JAR (A) 和 JEG-3 (B) 全细胞裂解液中 B7-H3 表达的 Western 印迹分析.
B7-H3 抗体 (F-11): sc-376769. 在 JAR (A) 和 JEG-3 (B) 全细胞裂解液中 B7-H3 表达的 Western 印迹分析.

B7-H3 抗体 (F-11): sc-376769

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寫評論提問

说明书
  • B7-H3 抗体 F-11 是小鼠单克隆 IgG2a κ，B7-H3抗体, 在18篇文献中引用，规格为200 µg/ml
  • 针对B7-H3的human起源的N-terminal细胞外域内的氨基酸166-465
  • B7-H3 抗体 (F-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 B7-H3
  • 抗B7-H3抗体（F-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取B7-H3 (F-11): sc-376769的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成B7-H3 抗体 (F-11)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

B7-H3抗体（F-11）是一种IgG2a κ小鼠单克隆B7-H3抗体（也称为B7-H3抗体），它通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的B7-H3蛋白。B7-H3抗体（F-11）既可作为非偶联抗B7-H3抗体形式提供，也可作为多种偶联形式的抗B7-H3抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。T细胞的活化和免疫功能受到先天免疫系统通过正负共刺激分子的调节。其中一种分子B7-H3（B7同源物3，也称为B7RP-2）属于B7免疫球蛋白超家族。可溶性B7-H3与活化T细胞上的一个假定受体结合，该受体与CD28、CTLA-4、ICOS和PD-1不同。B7-H3在非淋巴组织上广泛表达，共同刺激CD4+和CD8+ T细胞的增殖。B7-H3刺激Th1和细胞毒性T细胞反应的能力表明，它可能具有抗肿瘤活性。B7-H3的相互作用可能在调节细胞介导的抗肿瘤免疫反应中发挥作用，这表明B7-H3可能是一种潜在的治疗工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

B7-H3 抗体 (F-11) 参考文献:

  1. B7-H3：T 细胞活化和产生 IFN-gamma 的激动分子。  |  Chapoval, AI., et al. 2001. Nat Immunol. 2: 269-74. PMID: 11224528
  2. 小鼠和人类 B7-H3 基因的特征。  |  Sun, M., et al. 2002. J Immunol. 168: 6294-7. PMID: 12055244
  3. T 淋巴细胞在与树突状细胞相互作用后会表达 B7 家族分子，并获得旁观者成本刺激特性。  |  Ferlazzo, G., et al. 2002. Eur J Immunol. 32: 3092-101. PMID: 12385029
  4. B7家族成员B7-H3优先下调T辅助1型介导的免疫反应。  |  Suh, WK., et al. 2003. Nat Immunol. 4: 899-906. PMID: 12925852
  5. 小鼠 B7-H3 可诱导抗肿瘤免疫。  |  Sun, X., et al. 2003. Gene Ther. 10: 1728-34. PMID: 12939639
  6. 小鼠 B7-H3 是 T 细胞的负调控因子。  |  Prasad, DV., et al. 2004. J Immunol. 173: 2500-6. PMID: 15294965
  7. 免疫调节蛋白 B7-H3 可促进成骨细胞分化和骨矿化。  |  Suh, WK., et al. 2004. Proc Natl Acad Sci U S A. 101: 12969-73. PMID: 15317945
  8. B7-H3 促进急性和慢性异体移植排斥反应。  |  Wang, L., et al. 2005. Eur J Immunol. 35: 428-38. PMID: 15682454
  9. B7-H3：Mo-DC 的另一个分子标记？  |  Zhang, G., et al. 2005. Cell Mol Immunol. 2: 307-11. PMID: 16274630
  10. mTORC1 上调 B7-H3/CD276 以抑制抗肿瘤 T 细胞并驱动肿瘤免疫逃避。  |  Liu, HJ., et al. 2023. Nat Commun. 14: 1214. PMID: 36869048
  11. 免疫检查点 B7-H3 是携带 PTEN 和 TP53 缺陷的前列腺癌的治疗漏洞。  |  Shi, W., et al. 2023. Sci Transl Med. 15: eadf6724. PMID: 37163614
  12. 肿瘤临床试验中的 B7-H3 抑制剂：综述。  |  Feustel, K., et al. 2024. J Immunother Precis Oncol. 7: 53-66. PMID: 38327753

订购信息

产品名称产品编号规格价格数量收藏夹

B7-H3 抗体 (F-11)

sc-376769
200 µg/ml
RMB2377.00

B7-H3 抗体 (F-11) AC

sc-376769 AC
500 µg/ml, 25% agarose
RMB3129.00

B7-H3 抗体 (F-11) HRP

sc-376769 HRP
200 µg/ml
RMB2377.00

B7-H3 抗体 (F-11) FITC

sc-376769 FITC
200 µg/ml
RMB2482.00

B7-H3 抗体 (F-11) PE

sc-376769 PE
200 µg/ml
RMB2580.00

B7-H3 抗体 (F-11) Alexa Fluor® 488

sc-376769 AF488
200 µg/ml
RMB2685.00

B7-H3 抗体 (F-11) Alexa Fluor® 546

sc-376769 AF546
200 µg/ml
RMB2685.00

B7-H3 抗体 (F-11) Alexa Fluor® 594

sc-376769 AF594
200 µg/ml
RMB2685.00

B7-H3 抗体 (F-11) Alexa Fluor® 647

sc-376769 AF647
200 µg/ml
RMB2685.00

B7-H3 抗体 (F-11) Alexa Fluor® 680

sc-376769 AF680
200 µg/ml
RMB2685.00

B7-H3 抗体 (F-11) Alexa Fluor® 790

sc-376769 AF790
200 µg/ml
RMB2685.00

In one of your figures, you show that this antibody works for B7H3 expression on Jurkats, so I'd like to use them as a control for my own blot. What were the conditions that these Jurkat cells were grown in/stimulated to be able to express B7H3?

Asked by: Diana
Thank you for contacting Santa Cruz Biotechnology. For assistance please contact Technical support. Please call 800-457-3801 or email [email protected].
Answered by: BlakeJ
Date published: 2025-07-23

I need to use an antibody to detect the protein in samples of bovine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 15
Date published: 2017-07-19
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Rated 4 out of 5 by from Works fine in WB and IPWorked in WB with 50 µg of CRC cell lines (RIPA cell lysates) (1:500-1:1000 in 5%Milk/TBST). Showing some background but protein band is clear. Worked also in IP (2.5 µg protein and 1 mg HCT116 cell lysate).
Date published: 2021-05-04
Rated 5 out of 5 by from Good antibodyI received a free sample of this antibody and I used it for WB analysis. I got excellent results for the detection of both human and mouse B7H3 isoforms. WB dilution 1:500 works well.
Date published: 2020-10-06
Rated 5 out of 5 by from Good antibodyI bought this antibody for WB ,IP and IHC.always got good results.Westernblot 1:800 dilution is great.
Date published: 2018-09-16
Rated 5 out of 5 by from 抗体不错抗体小样使用，感觉不错！一个月前买的，抗体稀释比例是一比五百，能够做几次wb，感觉不错，抗体特异性还行
Date published: 2017-09-11
Rated 5 out of 5 by from Broad detectionThis antibody will detect several B7-H3 isoforms in mouse, rat, and human samples. The immunostaining results turn out great with clear and specific signal. This product has been published.
Date published: 2017-01-27
Rated 5 out of 5 by from Produced excellent immunoperoxidase cytoplasmicProduced excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human seminal vesicle tissue. -SCBT QC
Date published: 2015-04-19
Rated 5 out of 5 by from Produced positive Western Blot data of B7Produced positive Western Blot data of B7-H3 expression in JAR and JEG-3 whole cell lysates. -SCBT QC
Date published: 2014-07-12
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B7-H3 抗体 (F-11) is rated 4.9 out of 5 by 7.
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