Date published: 2025-9-7
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B23/Nucleophosmin Antibody (NA24) - Western Blotting - Image 16152
B23 抗体 (NA24): sc-53175. B23 在 HEL 92. 1. 7 (A), DU 145 (B), HeLa (C), K-562 (D), CCRF-CEM (E) 和 MCF7 (F) 全细胞裂解液中的表达的 Western 印迹分析.
B23 抗体 (NA24): sc-53175. B23 在 HEL 92. 1. 7 (A), DU 145 (B), HeLa (C), K-562 (D), CCRF-CEM (E) 和 MCF7 (F) 全细胞裂解液中的表达的 Western 印迹分析.

B23/Nucleophosmin 抗体 (NA24): sc-53175

5.0(8)
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说明书
  • B23/Nucleophosmin抗体(NA24)是小鼠单克隆IgG1 κ, 在17篇文献中引用，规格为200 µg/ml
  • 针对与B23的human起源的N-terminus相对应的重组蛋白
  • 推荐用于 mouse, rat 和 human 来源的B23 WB, IP, IF 和 IHC(P)检测
  • 看 B23/Nucleophosmin (E-3): sc-271737，可查看有标记的 B23/Nucleophosmin 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 B23/Nucleophosmin 抗体 (NA24) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与B23/Nucleophosmin 抗体 (NA24) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Nucleolar phosphoprotein/nucleophosmin抗体

蛋白质穿过核膜的运输是一个选择性的多步骤过程，涉及多种细胞质因子。蛋白质必须被识别为输入底物，停靠在核孔复合体上，并以ATP依赖的方式穿过核膜。已鉴定出几个对这一过程至关重要的细胞质和核蛋白。例如，在识别和停靠过程中至关重要的两个细胞质因子是核转运蛋白α和核转运蛋白β蛋白。核转运蛋白全酶是α和β亚基的异二聚体。核蛋白B23（也称为核磷蛋白）参与核糖体组装和rRNA运输。B23是一种丰富的蛋白质，在有丝分裂过程中被Cdc2激酶高度磷酸化。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

B23/Nucleophosmin 抗体 (NA24)

sc-53175
200 µg/ml
RMB2377.00

B23/Nucleophosmin (NA24): m-IgG Fc BP-HRP 套装

sc-536903
200 µg Ab; 10 µg BP
RMB2662.00

B23/Nucleophosmin (NA24): m-IgGκ BP-HRP 套装

sc-534075
200 µg Ab; 40 µg BP
RMB2662.00

B23/Nucleophosmin (NA24): m-IgG1 BP-HRP 套装

sc-544887
200 µg Ab; 20 µg BP
RMB2662.00

I am using B23 (NA24): sc-53175 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenniferc
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from An antibody that will make you happy!The nucleolin (B23) is always my favorite protein because of its distribution in the cell, it gives punctuate spots in the nucleus which looks great in immuno-fluorescence. The antibody gave clean bands at around 40kda in human cell lines as well as mouse cell line(see the blot). Which is expected size of the proteins, the additional bands may be isoforms or post transnational modification of the protein. Highly recommend this antibody.
Date published: 2017-11-14
Rated 5 out of 5 by from For WBFor WB, dilution 1:1000 gives a very good signal.
Date published: 2016-06-28
Rated 5 out of 5 by from Published IHC data for use in human bonePublished IHC data for use in human bone. -SCBT Publication Review
Date published: 2015-06-04
Rated 5 out of 5 by from Published data for IF in HeLa cellsPublished data for IF in HeLa cells. -SCBT Publication Review
Date published: 2015-05-01
Rated 5 out of 5 by from Exceptional immunoperoxidase nucleolar andExceptional immunoperoxidase nucleolar and nuclear staining in formalin fixed, paraffin-embedded human vulva/anal skin tissue. -SCBT QC
Date published: 2015-04-08
Rated 5 out of 5 by from Worked well for WB in human B23 transfectedWorked well for WB in human B23 transfected 293 whole cell lysate. -SCBT QC
Date published: 2015-02-19
Rated 5 out of 5 by from Clean band observed in WB in HeLa whole cellClean band observed in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-02-19
Rated 5 out of 5 by from Band of interest observed in WB in nonBand of interest observed in WB in non-transfected 293T, human B23 transfected 293T and HEL 92.1.7 whole cell lysates. -SCBT QC
Date published: 2015-01-31
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B23/Nucleophosmin 抗体 (NA24) is rated 5.0 out of 5 by 8.
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