Date published: 2025-9-7

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B23/Nucleophosmin 抗体 (0412): sc-47725

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说明书
  • B23/Nucleophosmin抗体(0412)是小鼠单克隆IgG1 κ, 在23篇文献中引用,规格为200 µg/ml
  • 免疫human物种的重组B23
  • B23/Nucleophosmin 抗体 (0412) 推荐用于WB, IP, IF 和 IHC(P),检测human 来源的 B23
  • 抗B23/Nucleophosmin抗体(0412)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取B23/Nucleophosmin (0412): sc-47725免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 B23/Nucleophosmin 抗体 (0412) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与B23/Nucleophosmin 抗体 (0412) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

B23抗体(0412)是一种小鼠单克隆IgG1κ轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和石蜡包埋切片免疫组织化学(IHCP)检测人类来源的B23(也称为核磷蛋白)。B23 (0412) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。B23在核糖体组装和核糖体RNA运输中发挥着关键作用,而核糖体组装和核糖体RNA运输是蛋白质合成和细胞功能所必需的过程。B23主要位于核仁中,参与核糖体亚基的成熟,而适当的定位对于维持有效的核糖体生物发生至关重要。在细胞分裂过程中,B23被Cdc2激酶高度磷酸化,这表明磷酸化状态可能调节细胞分裂过程中的功能和相互作用。蛋白质穿过核膜是一个选择性的多步骤过程,需要识别导入底物并在核孔复合物处对接,而B23是这一复杂机制中的关键角色。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

B23/Nucleophosmin 抗体 (0412) 参考文献:

  1. 核极蛋白 B23 具有分子伴侣活性。  |  Szebeni, A. and Olson, MO. 1999. Protein Sci. 8: 905-12. PMID: 10211837
  2. B23 可调节 GADD45a 的核转位,并有助于 GADD45a 诱导的细胞周期 G2-M 停滞。  |  Gao, H., et al. 2005. J Biol Chem. 280: 10988-96. PMID: 15644315
  3. 以 B23 相互作用序列为工具可视化细胞环境中的蛋白质相互作用  |  Lechertier, T., et al. 2007. J Cell Sci. 120: 265-75. PMID: 17179202
  4. B23/nucleophosmin 在感染后期参与腺病毒染色质结构的调节,但不参与病毒复制和转录。  |  Samad, MA., et al. 2012. J Gen Virol. 93: 1328-1338. PMID: 22337638
  5. 醋酸泽泻醇 B23 抑制肺癌的机制: 靶向调节 CD11b/CD18 影响巨噬细胞极化。  |  Chen, Y., et al. 2022. Drug Des Devel Ther. 16: 3677-3689. PMID: 36277599
  6. 蛋白质从细胞核输出需要 GTP 酶 Ran 和 GTP 水解。  |  Moroianu, J. and Blobel, G. 1995. Proc Natl Acad Sci U S A. 92: 4318-22. PMID: 7753805
  7. 细胞周期阶段依赖于核ophosmin / B23 的定域和寡聚状态的变化。  |  Chou, YH. and Yung, BY. 1995. Biochem Biophys Res Commun. 217: 313-25. PMID: 8526928
  8. Ran 结合域促进 Ran 与 p97/beta karyopherin 的相互作用,将核导入的对接和转运步骤联系起来。  |  Lounsbury, KM., et al. 1996. J Biol Chem. 271: 2357-60. PMID: 8576188
  9. karyopherin alpha 与 karyopherin beta 的结合位点与核定域序列重叠。  |  Moroianu, J., et al. 1996. Proc Natl Acad Sci U S A. 93: 6572-6. PMID: 8692858
  10. 核蛋白导入:Ran-GTP通过将α从β上的重叠结合位点移位,使核糖核蛋白α-β异二聚体解离。  |  Moroianu, J., et al. 1996. Proc Natl Acad Sci U S A. 93: 7059-62. PMID: 8692944
  11. 在石硫合剂诱导的染色体脱落过程中,有丝分裂特异形式的核分裂素/B23的积累和去磷酸化减少。  |  Lu, YY., et al. 1996. Biochem J. 317 (Pt 1): 321-7. PMID: 8694782
  12. 正常和肿瘤性结直肠粘膜中 nucleophosmin/B23 的表达。  |  Nozawa, Y., et al. 1996. J Pathol. 178: 48-52. PMID: 8778315

订购信息

产品名称产品编号规格价格数量收藏夹

B23/Nucleophosmin 抗体 (0412)

sc-47725
200 µg/ml
RMB2377.00

B23/Nucleophosmin (0412): m-IgG Fc BP-HRP 套装

sc-526734
200 µg Ab; 10 µg BP
RMB2662.00

B23/Nucleophosmin (0412): m-IgG1 BP-HRP 套装

sc-532107
200 µg Ab; 20 µg BP
RMB2662.00

B23/Nucleophosmin 抗体 (0412) AC

sc-47725 AC
500 µg/ml, 25% agarose
RMB3129.00

B23/Nucleophosmin 抗体 (0412) HRP

sc-47725 HRP
200 µg/ml
RMB2377.00

B23/Nucleophosmin 抗体 (0412) FITC

sc-47725 FITC
200 µg/ml
RMB2482.00

B23/Nucleophosmin 抗体 (0412) PE

sc-47725 PE
200 µg/ml
RMB2580.00

B23/Nucleophosmin 抗体 (0412) Alexa Fluor® 488

sc-47725 AF488
200 µg/ml
RMB2685.00

B23/Nucleophosmin 抗体 (0412) Alexa Fluor® 546

sc-47725 AF546
200 µg/ml
RMB2685.00

B23/Nucleophosmin 抗体 (0412) Alexa Fluor® 594

sc-47725 AF594
200 µg/ml
RMB2685.00

B23/Nucleophosmin 抗体 (0412) Alexa Fluor® 647

sc-47725 AF647
200 µg/ml
RMB2685.00

B23/Nucleophosmin 抗体 (0412) Alexa Fluor® 680

sc-47725 AF680
200 µg/ml
RMB2685.00

B23/Nucleophosmin 抗体 (0412) Alexa Fluor® 790

sc-47725 AF790
200 µg/ml
RMB2685.00

I am using B23 (0412): sc-47725 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Lower concentration is betterLower concentration is better (for accurate quantification) I have used as low as 1:5000 for lysates of some human cell lines.
Date published: 2017-02-02
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of B23 expression in CCRF-CEM, MCF7, Jurkat, A-431, PC-3 and HeLa whole cell lysates.
Date published: 2017-01-19
Rated 4 out of 5 by from Good for WBthe antibody works very well in WB but I can't detect any positive signal for ChIP-qPCR. So it's good for WB but not for ChIP.
Date published: 2017-01-10
Rated 5 out of 5 by from Published data for IP and WB in HeLa cellsPublished data for IP and WB in HeLa cells. -SCBT Publication Review
Date published: 2015-05-28
Rated 5 out of 5 by from StrongStrong, clear band obtained in WB in K-562 whole cell lysate. -SCBT QC
Date published: 2015-04-25
Rated 5 out of 5 by from Band of interest seen in WB in HEL 92Band of interest seen in WB in HEL 92.1.7 whole cell lysate. -SCBT QC
Date published: 2015-04-19
Rated 5 out of 5 by from Strong band detected in WB in human B23 transfectedStrong band detected in WB in human B23 transfected 293 whole cell lysate. -SCBT QC
Date published: 2015-04-18
Rated 5 out of 5 by from Good specicity in WB in human B23 transfectedGood specicity in WB in human B23 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-03-26
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B23/Nucleophosmin 抗体 (0412) is rated 4.9 out of 5 by 11.
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