Date published: 2025-9-7
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B23/Nucleophosmin Antibody (E-3) - Western Blotting - Image 377251
B23 抗体 (E-3): sc-271737. F9 (A), c4 (B), Caco-2 (C) 和 Daudi (D) 全细胞裂解液及 HL-60 核提取物 (E) 中 B23 表达的 Western 印迹分析.
B23 抗体 (E-3): sc-271737. F9 (A), c4 (B), Caco-2 (C) 和 Daudi (D) 全细胞裂解液及 HL-60 核提取物 (E) 中 B23 表达的 Western 印迹分析.

B23/Nucleophosmin 抗体 (E-3): sc-271737

4.1(13)
寫評論提問

说明书
  • B23/Nucleophosmin 抗体 E-3 是小鼠单克隆 IgG1 κ，B23/Nucleophosmin抗体, 在17篇文献中引用，规格为200 µg/ml
  • 特定于 human 起源的 B23 的 C-terminus (h) 处的氨基酸 253-294 之间的表位图谱
  • B23/Nucleophosmin 抗体 (E-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 B23; 也和以下物种反应，包括： 和 equine, canine, bovine and porcine
  • 抗B23/Nucleophosmin抗体（E-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取B23/Nucleophosmin (E-3): sc-271737的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是B23/Nucleophosmin Antibody (E-3) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与B23/Nucleophosmin Antibody (E-3) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Nucleolar phosphoprotein/nucleophosmin抗体

B23抗体（E-3）是IgG1 κ小鼠单克隆B23抗体（也称为NPM1抗体或核磷蛋白1抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人的B23蛋白。B23抗体（E-3）既可以作为非共轭抗B23抗体形式，也可以作为抗B23抗体的多种共轭形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。蛋白质跨核膜的转运是一个选择性的、多步骤的过程，涉及多种细胞质因子。蛋白质必须被识别为导入底物，停靠在核孔复合物上，并以ATP依赖的方式跨核膜转运。已经确定了几种对这一过程至关重要的胞质和核蛋白。例如，在识别和对接过程中起关键作用的两种胞质因子是karyopherin α和karyopherin β蛋白。karyopherin全酶是α和β亚基的异二聚体。核蛋白B23（也称为核磷蛋白）参与核糖体组装和rRNA转运。B23是一种丰富的蛋白质，在有丝分裂过程中被Cdc2激酶高度磷酸化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

B23/Nucleophosmin 抗体 (E-3)

sc-271737
200 µg/ml
RMB2377.00

B23/Nucleophosmin (E-3): m-IgG Fc BP-HRP 套装

sc-529252
200 µg Ab; 10 µg BP
RMB2662.00

B23/Nucleophosmin (E-3): m-IgGκ BP-HRP 套装

sc-522044
200 µg Ab, 40 µg BP
RMB2662.00

B23/Nucleophosmin (E-3): m-IgG1 BP-HRP 套装

sc-543417
200 µg Ab; 20 µg BP
RMB2662.00

B23/Nucleophosmin 抗体 (E-3) AC

sc-271737 AC
500 µg/ml, 25% agarose
RMB3129.00

B23/Nucleophosmin 抗体 (E-3) HRP

sc-271737 HRP
200 µg/ml
RMB2377.00

B23/Nucleophosmin 抗体 (E-3) FITC

sc-271737 FITC
200 µg/ml
RMB2482.00

B23/Nucleophosmin 抗体 (E-3) PE

sc-271737 PE
200 µg/ml
RMB2580.00

B23/Nucleophosmin 抗体 (E-3) Alexa Fluor® 488

sc-271737 AF488
200 µg/ml
RMB2685.00

B23/Nucleophosmin 抗体 (E-3) Alexa Fluor® 546

sc-271737 AF546
200 µg/ml
RMB2685.00

B23/Nucleophosmin 抗体 (E-3) Alexa Fluor® 594

sc-271737 AF594
200 µg/ml
RMB2685.00

B23/Nucleophosmin 抗体 (E-3) Alexa Fluor® 647

sc-271737 AF647
200 µg/ml
RMB2685.00

B23/Nucleophosmin 抗体 (E-3) Alexa Fluor® 680

sc-271737 AF680
200 µg/ml
RMB2685.00

B23/Nucleophosmin 抗体 (E-3) Alexa Fluor® 790

sc-271737 AF790
200 µg/ml
RMB2685.00

B23/Nucleophosmin (E-3) 中和勝肽

sc-271737 P
100 µg/0.5 ml
RMB511.00

Hi there, How could I get FREE 10 µg sample of B23 (E-3): sc-271737. I wish to test it on WB against B23 homologs in various distantly related species. I want to fist see If it works for me before making the purchase. Thanks  

Asked by: Shan_2018
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

What is the total volume provided of B23 (E-3):sc-271737 monoclonal antibody?

Asked by: jenni
Thank you for your question. This antibody is provided as 200 µg in 1 ml total volume of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support 7
Date published: 2017-01-13

Can B23 (E-3): sc-271737 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, B23 (E-3): sc-271737 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 7
Date published: 2017-01-13
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Rated 4 out of 5 by from suit for WBThis antibody is suitable for WB, but it cannot do IP
Date published: 2019-10-03
Rated 2 out of 5 by from OkayI tried to use for native PAGE WB; the results were okay but not a great as those obtained using the now discontinued B23 (C-19) antibody.
Date published: 2018-07-16
Rated 3 out of 5 by from Acceptable resultAdequate WB, not excellent, but acceptable result.
Date published: 2017-10-27
Rated 1 out of 5 by from Weak signal for IFI tried 1: 30 to 1000, but any dilution showed good staining for IF.
Date published: 2017-07-27
Rated 4 out of 5 by from great antibody for WBThis antibody works very well for WB (1:500 dilution). It also works for IF at 1:200 dilution (I suspect the signal would be stronger at lower dilutions).
Date published: 2017-07-05
Rated 5 out of 5 by from Good specificity in WBThis antibody shows a very good specificity with a dilution ratio 1:2000 in HeLa and MCF-7 cells.
Date published: 2017-06-27
Rated 5 out of 5 by from antibody for Western blotting.Strong and reliable bands seen on Western blot, is good
Date published: 2017-05-20
Rated 5 out of 5 by from Wow !! Outstanding antibodyBest available product for IHC staining, IF and WB studies
Date published: 2017-01-19
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B23/Nucleophosmin 抗体 (E-3) is rated 4.1 out of 5 by 13.
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