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B23/Nucleophosmin Antibody (3F291) - Western Blotting - Image 16153
B23 抗体 (3F291): sc-70392. B23 在 HEL 92. 1. 7 (A), DU 145 (B), HeLa (C), K-562 (D), CCRF-CEM (E) 和 MCF7 (F) 全细胞裂解液中的表达的 Western 印迹分析.
B23 抗体 (3F291): sc-70392. B23 在 HEL 92. 1. 7 (A), DU 145 (B), HeLa (C), K-562 (D), CCRF-CEM (E) 和 MCF7 (F) 全细胞裂解液中的表达的 Western 印迹分析.

B23/Nucleophosmin 抗体 (3F291): sc-70392

3.4(5)
寫評論提問

说明书
  • B23/Nucleophosmin抗体(3F291)是小鼠单克隆IgG1 κ, 在9篇文献中引用，规格为200 µg/ml
  • 针对与B23的human起源的N-terminus相对应的重组蛋白
  • 推荐用于 mouse, rat 和 human 来源的B23 WB, IP, IF 和 IHC(P)检测
  • 看 B23/Nucleophosmin (E-3): sc-271737，可查看有标记的 B23/Nucleophosmin 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 B23/Nucleophosmin 抗体 (3F291) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与B23/Nucleophosmin 抗体 (3F291) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Nucleolar phosphoprotein/nucleophosmin抗体

蛋白质穿过核膜的运输是一个选择性的多步骤过程，涉及多种细胞质因子。蛋白质必须被识别为输入底物，停靠在核孔复合体上，并以ATP依赖的方式穿过核膜。已鉴定出几个对这一过程至关重要的细胞质和核蛋白。例如，在识别和停靠过程中至关重要的两个细胞质因子是核转运蛋白α和核转运蛋白β蛋白。核转运蛋白全酶是α和β亚基的异二聚体。核蛋白B23（也称为核磷蛋白）参与核糖体组装和rRNA运输。B23是一种丰富的蛋白质，在有丝分裂过程中被Cdc2激酶高度磷酸化。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

B23/Nucleophosmin 抗体 (3F291)

sc-70392
200 µg/ml
RMB2377.00

B23/Nucleophosmin (3F291): m-IgG Fc BP-HRP 套装

sc-539270
200 µg Ab; 10 µg BP
RMB2662.00

B23/Nucleophosmin (3F291): m-IgG1 BP-HRP 套装

sc-541368
200 µg Ab; 20 µg BP
RMB2662.00

What is the recommended starting dilution for Western blot for sc-70392: B23 (3F291) monoclonal antibody?

Asked by: Professor Griffin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 1 out of 5 by from Does not work for IF in mouse neruonsTested free sample on ICC with dilution of 1:25 and 1:100 in primary neurons from mouse cortex. Does not work at all for ICC
Date published: 2018-12-05
Rated 4 out of 5 by from Positive result in WB in human B23 transfectedPositive result in WB in human B23 transfected 293T and HEL 92.1.7 whole cell lysates. -SCBT QC
Date published: 2015-05-05
Rated 4 out of 5 by from Good specificity in WB in HeLa whole cell lysateGood specificity in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-01-14
Rated 4 out of 5 by from Published data for IP in HEK cellsPublished data for IP in HEK cells. -SCBT Publication Review
Date published: 2015-01-11
Rated 4 out of 5 by from Band of interest observed in WB in humanBand of interest observed in WB in human B23 transfected 293 whole cell lysate. -SCBT QC
Date published: 2013-01-12
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B23/Nucleophosmin 抗体 (3F291) is rated 3.4 out of 5 by 5.
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