Date published: 2025-9-7

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Atg3 抗体 (D-9): sc-393623

4.5(2)
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说明书
  • Atg3抗体(D-9)是小鼠单克隆IgG1 κ, 在1篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列1-300位于human物种的Atg3的N-terminus
  • 推荐用于 mouse, rat 和 human 来源的Atg3 WB, IP, IF 和 ELISA检测
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是Atg3 Antibody (D-9) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Atg3 Antibody (D-9) 打包提供(请参阅下面的订购信息)。

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関連項目

Atg3(自噬相关蛋白3),也被称为APG3-like、hAPG3或PC3-96,是一种定位于细胞质的E2样酶,在多种组织中表达,主要在肾、胎盘、肝、心和骨骼肌中表达水平较高。Atg3催化Atg8-磷脂酰乙醇胺(Atg8-PE)偶联物的形成,这一反应对自噬(一种允许细胞器和大块细胞蛋白降解的细胞过程)至关重要。形成Atg8-PE偶联物的过程始于Atg4(一种半胱氨酸蛋白酶)去除Atg8的C端精氨酸残基。随后,现在暴露出来的甘氨酸残基被Atg7激活,然后转移到Atg3进行最终的与PE的偶联。Atg12-Atg5偶联物的存在可以加速这最后一步,它的功能类似于E3酶。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Atg3 抗体 (D-9) 参考文献:

  1. 鉴定高等真核生物中与 ATG7 相互作用的 ATG3 线性区域并确定其特征。  |  Ohashi, K. and Otomo, T. 2015. Biochem Biophys Res Commun. 463: 447-52. PMID: 26043688
  2. MicroRNA-495通过靶向ATG3调节饥饿诱导的自噬。  |  Li, W., et al. 2016. FEBS Lett. 590: 726-38. PMID: 26910393
  3. ATG3 依赖性自噬在多能性获得和维持过程中介导线粒体稳态。  |  Liu, K., et al. 2016. Autophagy. 12: 2000-2008. PMID: 27575019
  4. 人类 ATG3 与脂质双分子层的结合:脂质几何形状和电荷的作用。  |  Hervás, JH., et al. 2017. Sci Rep. 7: 15614. PMID: 29142222
  5. Atg3 和 Atg7 的氧化介导了自噬的抑制作用。  |  Frudd, K., et al. 2018. Nat Commun. 9: 95. PMID: 29311554
  6. Atg12-Atg3 协调基础自噬、溶酶体内转运和外泌体释放  |  Murrow, L. and Debnath, J. 2018. Mol Cell Oncol. 5: e1039191. PMID: 30263931
  7. Atg3 通过改变脂质扩散和重排促进 Atg8 脂化。  |  Wang, S., et al. 2020. Protein Sci. 29: 1511-1523. PMID: 32277540
  8. 通过敲除 ATG3 抑制自噬可缓解氧-葡萄糖剥夺/再氧诱导的脑微血管内皮细胞损伤和炎症。  |  Peng, Z., et al. 2021. Neurochem Res. 46: 3200-3212. PMID: 34379294
  9. 确定一种针对 ATG12-ATG3 蛋白-蛋白相互作用的自噬选择性抑制剂。  |  Nuta, GC., et al. 2023. Autophagy. 19: 2372-2385. PMID: 37184247
  10. ATG3 蛋白具有独特的两性螺旋,对 Atg8/LC3 脂化反应至关重要。  |  Nishimura, T., et al. 2024. Autophagy. 20: 212-213. PMID: 37679935

订购信息

产品名称产品编号规格价格数量收藏夹

Atg3 抗体 (D-9)

sc-393623
200 µg/ml
RMB2377.00

Atg3 (D-9): m-IgG Fc BP-HRP 套装

sc-538262
200 µg Ab; 10 µg BP
RMB2662.00

Atg3 (D-9): m-IgGκ BP-HRP 套装

sc-535839
200 µg Ab; 40 µg BP
RMB2662.00

Atg3 (D-9): m-IgG1 BP-HRP 套装

sc-545607
200 µg Ab; 20 µg BP
RMB2662.00

Are there any issues with using sc-393623: Atg3 (D-9) mouse monoclonal primary antibody on mouse samples?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-393623 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 4 out of 5 by from BetterI got a sample and it worked better for my western blots
Date published: 2019-01-25
Rated 5 out of 5 by from Very good Western blot data of Atg3 expressionVery good Western blot data of Atg3 expression in Jurkat, K-562, HeLa and Hep G2 whole cell lysates. -SCBT QC
Date published: 2014-07-01
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Atg3 抗体 (D-9) is rated 4.5 out of 5 by 2.
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