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Atg3 抗体 (A-3): sc-393660

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说明书
  • Atg3 抗体 A-3 是小鼠单克隆 IgG2b κ,Atg3抗体, 在16篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列1-300位于human物种的Atg3的N-terminus
  • Atg3 抗体 (A-3) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Atg3
  • 抗Atg3抗体(A-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Atg3 (A-3): sc-393660免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成Atg3 抗体 (A-3)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

Atg3抗体(A-3)是一种IgG2b κ小鼠单克隆Atg3抗体(也称为Atg3抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的Atg3蛋白。Atg3抗体(A-3)既有非偶联的抗Atg3抗体形式,也有多种偶联形式的抗Atg3抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Atg3(自噬相关蛋白3),也被称为APG3-like、hAPG3或PC3-96,是一种定位于细胞质的E2样酶,在多种组织中表达,主要在肾、胎盘、肝、心和骨骼肌中表达水平较高。Atg3催化Atg8-磷脂酰乙醇胺(Atg8-PE)偶联物的形成,这一反应对自噬(一种允许细胞器和大块细胞蛋白降解的细胞过程)至关重要。形成Atg8-PE偶联物的过程始于Atg4(一种半胱氨酸蛋白酶)去除Atg8的C端精氨酸残基。随后暴露出来的甘氨酸残基被Atg7激活,然后转移到Atg3进行最终的与PE的偶联。Atg12-Atg5偶联物的存在可以加速这最后一步,它的功能类似于E3酶。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Atg3 抗体 (A-3) 参考文献:

  1. 人类 Apg3p/Aut1p 同源物是多种底物(GATE-16, GABARAP 和 MAP-LC3)的真实 E2 酶,可促进 hApg12p 与 hApg5p 连接。  |  Tanida, I., et al. 2002. J Biol Chem. 277: 13739-44. PMID: 11825910
  2. 核糖核酸对 ATG12-ATG5 连接的刺激。  |  Shao, Y., et al. 2007. Autophagy. 3: 10-6. PMID: 16963840
  3. Atg3 的结晶和初步 X 射线分析。  |  Yamada, Y., et al. 2006. Acta Crystallogr Sect F Struct Biol Cryst Commun. 62: 1016-7. PMID: 17012800
  4. 大鼠 Apg3p/Aut1p 同源物在视网膜缺血预处理过程中上调。  |  Wu, BX., et al. 2006. Mol Vis. 12: 1292-302. PMID: 17110912
  5. Atg3的晶体结构,Atg3是一种自噬相关泛素载体蛋白(E2)酶,可介导Atg8脂化。  |  Yamada, Y., et al. 2007. J Biol Chem. 282: 8036-43. PMID: 17227760
  6. 自噬相关基因的过表达抑制酵母丝状生长  |  Ma, J., et al. 2007. Autophagy. 3: 604-9. PMID: 17700056
  7. 原生寄生虫恩塔莫阿米巴侵袭体增殖和囊变过程中的自噬作用  |  Picazarri, K., et al. 2008. Infect Immun. 76: 278-88. PMID: 17923513
  8. Atg12-Atg5共轭物在自噬过程中具有类似E3的新型蛋白质脂化活性。  |  Hanada, T., et al. 2007. J Biol Chem. 282: 37298-302. PMID: 17986448
  9. 考虑 LC3 阴性对照和四色荧光蛋白-LC3 阴性对照的表达载体。  |  Tanida, I., et al. 2008. Autophagy. 4: 131-4. PMID: 18000393
  10. PTK2 介导的 ATG3 降解会阻碍癌细胞接受 DNA 损伤治疗。  |  Ma, K., et al. 2017. Autophagy. 13: 579-591. PMID: 28103122
  11. 自噬 E2 Atg3 中的一个开关元件介导整个脂化级联的异构调节。  |  Zheng, Y., et al. 2019. Nat Commun. 10: 3600. PMID: 31399562
  12. 人 Atg3 在水溶液和双细胞中的核磁共振分配。  |  Ye, Y., et al. 2021. Biomol NMR Assign. 15: 421-425. PMID: 34296398

订购信息

产品名称产品编号规格价格数量收藏夹

Atg3 抗体 (A-3)

sc-393660
200 µg/ml
RMB2377.00

Atg3 抗体 (A-3) AC

sc-393660 AC
500 µg/ml, 25% agarose
RMB3129.00

Atg3 抗体 (A-3) HRP

sc-393660 HRP
200 µg/ml
RMB2377.00

Atg3 抗体 (A-3) FITC

sc-393660 FITC
200 µg/ml
RMB2482.00

Atg3 抗体 (A-3) PE

sc-393660 PE
200 µg/ml
RMB2580.00

Atg3 抗体 (A-3) Alexa Fluor® 488

sc-393660 AF488
200 µg/ml
RMB2685.00

Atg3 抗体 (A-3) Alexa Fluor® 546

sc-393660 AF546
200 µg/ml
RMB2685.00

Atg3 抗体 (A-3) Alexa Fluor® 594

sc-393660 AF594
200 µg/ml
RMB2685.00

Atg3 抗体 (A-3) Alexa Fluor® 647

sc-393660 AF647
200 µg/ml
RMB2685.00

Atg3 抗体 (A-3) Alexa Fluor® 680

sc-393660 AF680
200 µg/ml
RMB2685.00

Atg3 抗体 (A-3) Alexa Fluor® 790

sc-393660 AF790
200 µg/ml
RMB2685.00

I need to use an antibody to detect the protein in samples of equine origin. Do you know if sc-393660: Atg3 (A-3) mouse monoclonal antibody will work?

Asked by: TinTin
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 5 out of 5 by from Excellent!Antibody tested in human fibroblasts by Western Blot: very good signal at the expected molecular weight. Absence of non-specific bands.
Date published: 2019-01-10
Rated 5 out of 5 by from works great for western blotIt works really well for western blot on mouse heart and liver tissue! The consistency and high quality of new Santa Cruz monoclonal antibodies I have tested is much better than in the past.
Date published: 2017-08-22
Rated 5 out of 5 by from excellentI used this antibody in the dilution 1:500, and got one single and sharp brand!
Date published: 2017-08-08
Rated 5 out of 5 by from Great antibodyThis Atg3 antibody is very clean antibody, my Western blot analysis shows only one single strong band that disappears after knockdown with CRISPR-cas9 genomic editing. Excellent work, very impressed.
Date published: 2017-07-19
Rated 5 out of 5 by from Worked great on western blot on mouse samplesWorked great on western blot on mouse samples in dendritic cells
Date published: 2017-07-19
Rated 5 out of 5 by from specific bandUse this antibody, I got specific band in WB in human kidney tissue extracts .It's a good choice to purchase it than other suppliers.
Date published: 2017-04-15
Rated 5 out of 5 by from It's really nice.We used Atg3 antibody Western blot analysis of expression in rat spleen tissue extract,the effect is quite good.Very efficient antibody indeed.
Date published: 2017-02-13
Rated 5 out of 5 by from Also good qualityworked well for WB in mouse samples with the dilution of 1:600.
Date published: 2017-02-11
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Atg3 抗体 (A-3) is rated 5.0 out of 5 by 10.
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