Date published: 2025-9-6
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ATF4 Antibody (B-3) - Immunohistochemistry - Image 352323
ATF-4 抗体 (B-3): sc-390063. 福尔马林固定, 石蜡包埋的人类胎盘组织的免疫过氧化物酶染色, 显示滋养细胞的胞浆染色.
ATF-4 抗体 (B-3): sc-390063. 福尔马林固定, 石蜡包埋的人类胎盘组织的免疫过氧化物酶染色, 显示滋养细胞的胞浆染色.

ATF4 抗体 (B-3): sc-390063

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说明书
  • ATF4 抗体 B-3 是小鼠单克隆 IgG2b κ，ATF4抗体, 在118篇文献中引用，规格为200 µg/ml
  • 免疫human物种的CREB-2的氨基酸1-290
  • ATF4 抗体 (B-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 ATF-4
  • 抗ATF4抗体（B-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-390063 X, 200 µg/0.1 ml)
  • 关于如何获取ATF4 (B-3): sc-390063的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 ATF4 抗体 (B-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ATF4 抗体 (B-3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 CREB 抗体包括 CREB-1 和 ATF-4

ATF-4抗体（B-3）是一种IgG2b κ小鼠单克隆ATF-4抗体（也称为ATF4抗体、cAMP依赖性转录因子ATF-4抗体、CREB-2抗体或cAMP反应元件结合蛋白2抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的ATF-4蛋白。ATF-4抗体（B-3）可以以非偶联抗ATF-4抗体的形式提供，也可以以多种偶联形式的抗ATF-4抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。真核基因的转录受序列特异性转录因子的调控，这些转录因子与模块化的顺式作用启动子和增强子元件结合。cAMP反应元件（CRE）是这类元件中研究得最好的一种，由回文八核苷酸TGACGTCA组成。在ATF/CREB家族中已经鉴定出几种CRE结合蛋白，其中特征最明显的包括CREB-1、ATF-4（也称为CREB-2）、ATF-1、ATF-2和ATF-3。这些蛋白质共享高度相关的COOH末端亮氨酸拉链二聚化和基本DNA结合域，但在其氨基末端域中高度分化。尽管ATF/CREB蛋白中的每一种似乎都能以其同源二聚体形式结合CRE，但其中的某些蛋白也能以异源二聚体形式结合，既在ATF/CREB家族内部，甚至与AP-1转录因子家族的成员结合。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ATF4 抗体 (B-3) 参考文献:

  1. 转录因子 Fos/Jun 和 ATF/CREB 的跨家族二聚化改变了 DNA 结合的特异性。  |  Hai, T. and Curran, T. 1991. Proc Natl Acad Sci U S A. 88: 3720-4. PMID: 1827203
  2. 人类环 AMP 反应元件结合蛋白的 cDNA，该蛋白与 CREB 不同，主要在大脑中表达。  |  Kara, CJ., et al. 1990. Mol Cell Biol. 10: 1347-57. PMID: 2320002
  3. 转录因子 ATF cDNA 克隆：能够选择性地形成 DNA 结合异二聚体的大量亮氨酸拉链蛋白家族。  |  Hai, TW., et al. 1989. Genes Dev. 3: 2083-90. PMID: 2516827
  4. 与大脑中环状 AMP 反应元件结合的 CRE-BP1 蛋白的亮氨酸拉链结构。  |  Maekawa, T., et al. 1989. EMBO J. 8: 2023-8. PMID: 2529117
  5. 一种常见的细胞转录因子 ATF 与 E1a 和环 AMP 诱导的启动子中的调控元件相互作用。  |  Lin, YS. and Green, MR. 1988. Proc Natl Acad Sci U S A. 85: 3396-400. PMID: 2835770
  6. 鉴定大鼠体生长抑素基因中的环-AMP 反应元件。  |  Montminy, MR., et al. 1986. Proc Natl Acad Sci U S A. 83: 6682-6. PMID: 2875459
  7. 环磷酸腺苷反应性 DNA 结合蛋白：基于克隆胎盘 cDNA 的结构。  |  Hoeffler, JP., et al. 1988. Science. 242: 1430-3. PMID: 2974179
  8. ATF-4和硫化氢信号传导可延长翻译或mTORC1抑制的寿命。  |  Statzer, C., et al. 2022. Nat Commun. 13: 967. PMID: 35181679
  9. PERK 是巨噬细胞免疫抑制功能的关键代谢枢纽。  |  Raines, LN., et al. 2022. Nat Immunol. 23: 431-445. PMID: 35228694
  10. β-（4-氟苄基）Arteannuin B诱导的ATF-4和C/EBPβ相互作用介导了乳腺癌细胞从自噬到衰老的转变。  |  Mir, KB., et al. 2022. Front Oncol. 12: 1013500. PMID: 36465376
  11. 大鼠大脑中的 CREB-1 和 CREB-2 免疫反应。  |  Ferrer, I., et al. 1996. Brain Res. 712: 159-64. PMID: 8705300
  12. CREB-2是一种依赖于细胞CRE的转录抑制因子，它与Tax一起发挥着人类T细胞白血病病毒1型启动子激活剂的功能。  |  Gachon, F., et al. 1998. J Virol. 72: 8332-7. PMID: 9733879

订购信息

产品名称产品编号规格价格数量收藏夹

ATF4 抗体 (B-3)

sc-390063
200 µg/ml
RMB2377.00

ATF4 (B-3): m-IgGκ BP-HRP 套装

sc-523391
200 µg Ab, 40 µg BP
RMB2662.00

ATF4 (B-3): m-IgG2b BP-HRP 套装

sc-549116
200 µg Ab; 10 µg BP
RMB2662.00

ATF4 抗体 (B-3) X

sc-390063 X
200 µg/0.1 ml
RMB2377.00

ATF4 抗体 (B-3) AC

sc-390063 AC
500 µg/ml, 25% agarose
RMB3129.00

ATF4 抗体 (B-3) HRP

sc-390063 HRP
200 µg/ml
RMB2377.00

ATF4 抗体 (B-3) FITC

sc-390063 FITC
200 µg/ml
RMB2482.00

ATF4 抗体 (B-3) PE

sc-390063 PE
200 µg/ml
RMB2580.00

ATF4 抗体 (B-3) Alexa Fluor® 488

sc-390063 AF488
200 µg/ml
RMB2685.00

ATF4 抗体 (B-3) Alexa Fluor® 546

sc-390063 AF546
200 µg/ml
RMB2685.00

ATF4 抗体 (B-3) Alexa Fluor® 594

sc-390063 AF594
200 µg/ml
RMB2685.00

ATF4 抗体 (B-3) Alexa Fluor® 647

sc-390063 AF647
200 µg/ml
RMB2685.00

ATF4 抗体 (B-3) Alexa Fluor® 680

sc-390063 AF680
200 µg/ml
RMB2685.00

ATF4 抗体 (B-3) Alexa Fluor® 790

sc-390063 AF790
200 µg/ml
RMB2685.00

I am enquiring on whether trial aliquots are available for this antibody for shipping to the UK (Northern Ireland)?

Asked by: tmcgirr
Thank you for contacting Santa Cruz Biotechnology. To request a sample please email [email protected].
Answered by: BlakeJ
Date published: 2025-03-07

Hi, this antibody will be compatible to WES ( Simple protein).? I am trying to see ATF4 in Mice.

Asked by: Bikko
Thank you for your question. Anti-CREB-2/ATF-4 Antibody (B-3): sc-390063 is recommended for detection of ATF-4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. It has not been tested in automated systems such as WES, but should generally be compatible.
Answered by: Tech Support Europe
Date published: 2022-09-29

Hello, is this antibody compatible with goat anti-mouse IgG (H+L) secondary antibody? Thanks

Asked by: scio
Thank you for your question. Yes, CREB-2 Antibody (B-3) is a mouse monoclonal IgG2b, which is suitable for detection using a goat anti-mouse IgG (H+L) secondary antibody.
Answered by: Technical Support
Date published: 2019-01-17

Hello, Hope all is well. I wanted to order the CREB-2 (B-3) AB SC-390063 it says the concentration is 200ug/mL, I was wondering if the total volume will be 1 mL of AB for $279? Thank you

Asked by: Nikki
Thank you for your question! Yes, this antibody is provided as 200µg in 1ml. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-03-01

Are there any issues with non-specific staining when using this antibody on mouse tissue in IF or IHC?

Asked by: AbPolly
Thank you for your question. This antibody is recommended for use with mouse samples for IF and IHC. To prevent any potential non-specific staining from an anti-mouse secondary, we recommend using a directly conjugated primary. This antibody is available conjugated to HRP, PE, FITC, AF488 and AF647
Answered by: Technical Support 7
Date published: 2017-01-25
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Rated 3 out of 5 by from Anti-ATF4 per analisi in western blotL'Ab è stato testato utilizzando un lisato cellulare di MDA-MB-231 ottenuto in RIPA buffer. Sono state utilizzate cellule controllo e cellule trattate con induttori dello stress del reticolo. 20 ug di proteine sono state separate tramite SDS-Page e trasferite su un filtro di nitrocellulosa. Il bloccaggio è stato effettuato in Blotto A, l'Ab primario è stato utilizzato alla diluizione di 1:1000 in 10 mL di Blotto A. Il secondario 1:2000 in Blotto A. Il segnale ottenuto è debole ma con un'unica banda del perso molecolare atteso.
Date published: 2024-11-10
Rated 5 out of 5 by from Mouse monoclonal ATF4 (Clone B-3)We used this antibody for WB and it worked well for our research.
Date published: 2024-01-29
Rated 4 out of 5 by from Good work!This antibody gave a strong and clean signal when we used it at a 1:100 diluition with 80μg of protein samples.
Date published: 2023-05-04
Rated 4 out of 5 by from WB以前那款兔抗用完了，申请了这款！大约在45-50kD附近有条带，或许有杂带，曝光时间可以短一点试试。推荐！
Date published: 2022-03-04
Rated 2 out of 5 by from Not detected at right sizeMultiple bands detected but none at expected size.
Date published: 2021-12-03
Rated 4 out of 5 by from It worked goodIt works good without background. The dilution I used was 1:500.
Date published: 2021-10-25
Rated 2 out of 5 by from 条带位置不明确前几天收到该抗体，立马做了实验，但是，出现了明显的三条带（40-55kDa之间），到底哪一条是呢？希望能够尽快得到回复，谢谢
Date published: 2020-10-11
Rated 5 out of 5 by from works goodVery good antibody. I used at 1:500 dilution and got a specific band at around 50kd.
Date published: 2020-10-08
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ATF4 抗体 (B-3) is rated 3.8 out of 5 by 31.
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