Date published: 2025-9-7
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ATAD3A/B/C Antibody (A-4) - Western Blotting - Image 377683
ATAD3A/B/C 抗体 (A-4): sc-376185. Jurkat (A), K-562 (B), HeLa (C) 和 A549 (D) 全细胞裂解液中 ATAD3A/B/C 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
ATAD3A/B/C 抗体 (A-4): sc-376185. Jurkat (A), K-562 (B), HeLa (C) 和 A549 (D) 全细胞裂解液中 ATAD3A/B/C 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

ATAD3A/B/C 抗体 (A-4): sc-376185

4.8(4)
寫評論提問

说明书
  • ATAD3A/B/C 抗体 A-4 是小鼠单克隆 IgG1 κ，ATAD3A/B/C抗体, 在8篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列407-634位于human物种的ATAD3A的C-terminus
  • ATAD3A/B/C 抗体 (A-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测 ATAD3A, ATAD3B and ATAD3C of human origin, and ATAD3A of mouse and rat origin
  • 抗ATAD3A/B/C抗体（A-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ATAD3A/B/C (A-4): sc-376185的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和m-IgGκ BP-HRP是ATAD3A/B/C Antibody (A-4) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂，这些试剂现与ATAD3A/B/C Antibody (A-4) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加ATAD抗体，包括ATAD3A, ATAD3B和ATAD3C

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ATAD3A/B/C 抗体 (A-4) 参考文献:

  1. AAA+ 超家族 ATP 酶：结构相同，功能各异。  |  Ogura, T. and Wilkinson, AJ. 2001. Genes Cells. 6: 575-97. PMID: 11473577
  2. AAA ATP 酶 Cdc48/p97 及其伙伴将蛋白质从 ER 转运到细胞质中。  |  Ye, Y., et al. 2001. Nature. 414: 652-6. PMID: 11740563
  3. AAA+ ATPases 的进化史和高阶分类。  |  Iyer, LM., et al. 2004. J Struct Biol. 146: 11-31. PMID: 15037234
  4. 对 1 号染色体进化的新认识。  |  Weise, A., et al. 2005. Cytogenet Genome Res. 108: 217-22. PMID: 15545733
  5. [AAA 蛋白超家族 ATP 酶的结构, 功能和作用机制]。  |  Kedzierska, S. 2006. Postepy Biochem. 52: 330-8. PMID: 17201069
  6. AAA+ 蛋白 ATAD3 具有位移环结合特性，参与线粒体核仁组织。  |  He, J., et al. 2007. J Cell Biol. 176: 141-6. PMID: 17210950
  7. ATAD 3A 和 ATAD 3B 是远端 1p 定位基因，在人类胶质瘤细胞系中表达不同，具有体外抗致癌和抗化疗特性。  |  Hubstenberger, A., et al. 2008. Exp Cell Res. 314: 2870-83. PMID: 18639545
  8. ATAD3B是一种线粒体吞噬受体，可清除氧化应激引起的受损线粒体DNA。  |  Shu, L., et al. 2021. EMBO J. 40: e106283. PMID: 33665835
  9. ATAD3A：线粒体相关疾病的关键调节因子。  |  Chen, L., et al. 2023. Int J Mol Sci. 24: PMID: 37569886
  10. "ATAD3C调节线粒体膜上ATAD3A的组装和功能"。  |  Gaudó, P., et al. 2024. Free Radic Biol Med. 211: 114-126. PMID: 38092275
  11. AAA 团队：具有不同功能的相关 ATP 酶。  |  Patel, S. and Latterich, M. 1998. Trends Cell Biol. 8: 65-71. PMID: 9695811
  12. AAA+：一类类似于伴侣的 ATP 酶，与蛋白质复合物的缔合, 运行和分解有关。  |  Neuwald, AF., et al. 1999. Genome Res. 9: 27-43. PMID: 9927482

订购信息

产品名称产品编号规格价格数量收藏夹

ATAD3A/B/C 抗体 (A-4)

sc-376185
200 µg/ml
RMB2377.00

ATAD3A/B/C (A-4): m-IgG Fc BP-HRP 套装

sc-529831
200 µg Ab; 10 µg BP
RMB2662.00

ATAD3A/B/C (A-4): m-IgGκ BP-HRP 套装

sc-522866
200 µg Ab, 40 µg BP
RMB2662.00

ATAD3A/B/C 抗体 (A-4) AC

sc-376185 AC
500 µg/ml, 25% agarose
RMB3129.00

ATAD3A/B/C 抗体 (A-4) HRP

sc-376185 HRP
200 µg/ml
RMB2377.00

ATAD3A/B/C 抗体 (A-4) FITC

sc-376185 FITC
200 µg/ml
RMB2482.00

ATAD3A/B/C 抗体 (A-4) PE

sc-376185 PE
200 µg/ml
RMB2580.00

ATAD3A/B/C 抗体 (A-4) Alexa Fluor® 488

sc-376185 AF488
200 µg/ml
RMB2685.00

ATAD3A/B/C 抗体 (A-4) Alexa Fluor® 546

sc-376185 AF546
200 µg/ml
RMB2685.00

ATAD3A/B/C 抗体 (A-4) Alexa Fluor® 594

sc-376185 AF594
200 µg/ml
RMB2685.00

ATAD3A/B/C 抗体 (A-4) Alexa Fluor® 647

sc-376185 AF647
200 µg/ml
RMB2685.00

ATAD3A/B/C 抗体 (A-4) Alexa Fluor® 680

sc-376185 AF680
200 µg/ml
RMB2685.00

ATAD3A/B/C 抗体 (A-4) Alexa Fluor® 790

sc-376185 AF790
200 µg/ml
RMB2685.00

What is the recommended fixation method for immunofluorescence staining with ATAD3A/B/C (A-4): sc-376185 monoclonal antibody?

Asked by: TinTin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from good signalHeLa cells fixed with formaldehyde 3.7%, anti ATAD3A/B/C 1/100 (green) + anti TOMM20 (red), 4°C O/N.
Date published: 2025-08-26
Rated 4 out of 5 by from Good working AbWe tried this antibody in WB on Huh7 cell lysates at a concentration of 1:1000. We were able to obtain a defined band at the predicted molecular weight.
Date published: 2023-07-05
Rated 5 out of 5 by from Good signalGood signal for western blot. Samples: Whole cell lysates and purified mitochondria from HEK 293T cells. Used at 1:1000 dilution in TTBS with 5% milk, over night at 4ºC. Gives a clear band at the expected size (70KDa), between 50KDa and 75KDa marker bands.
Date published: 2020-11-17
Rated 5 out of 5 by from Produced great immunofluorescence mitochondrialProduced great immunofluorescence mitochondrial staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-21
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ATAD3A/B/C 抗体 (A-4) is rated 4.8 out of 5 by 5.
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