Date published: 2025-9-7
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Arp2 Antibody (E-12) - Western Blotting - Image 356551
Arp2 抗体 (E-12): sc-166103. A-673 (A), Hs 732. Sk/Mu (B), C2C12 (C), Sol8 (D), L8 (E) 和 A-10 (F) 全细胞裂解液中 Arp2 表达的 Western 印迹分析.
Arp2 抗体 (E-12): sc-166103. A-673 (A), Hs 732. Sk/Mu (B), C2C12 (C), Sol8 (D), L8 (E) 和 A-10 (F) 全细胞裂解液中 Arp2 表达的 Western 印迹分析.

Arp2 抗体 (E-12): sc-166103

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说明书
  • Arp2 抗体 E-12 是小鼠单克隆 IgG1 κ，Arp2抗体, 在9篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Arp2的氨基酸311-394
  • Arp2 抗体 (E-12) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ，检测 mouse, rat, human 和 来源的Arp2; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 抗Arp2抗体（E-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Arp2 (E-12): sc-166103的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Arp2 抗体 (E-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Arp2 抗体 (E-12) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Actin Related Protein 抗体包括 ACTR10, Arp2, Arp3, ACTR5 和 ACTR6

Arp2抗体（E-12）是一种IgG1κ小鼠单克隆Arp2抗体（也称为肌动蛋白相关蛋白2抗体、ARP2肌动蛋白相关蛋白2同源抗体、肌动蛋白相关蛋白2抗体或肌动蛋白样蛋白2抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的Arp2蛋白。Arp2抗体（E-12）可作为非偶联抗Arp2抗体形式和多种偶联形式的抗Arp2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。肌动蛋白聚合是细胞执行多种功能所必需的，这些功能包括趋化性、细胞迁移、细胞粘附和血小板激活等。细胞通过以下方式触发肌动蛋白聚合：从单体肌动蛋白中重新形成丝状核、切断现有丝状物以产生未封端的带刺末端，或使现有的带刺末端去封端。肌动蛋白成核是肌动蛋白聚合中的限速和不利反应，因此需要Arp2/3复合物的参与。Arp2/3复合物有助于形成新的丝状物，并促进肌动蛋白丝以端到侧的方式交联成分支网状结构。Arp2/3复合物由与肌动蛋白相关的蛋白Arp2和Arp3，以及其他各种辅助蛋白组成。Arp2/3复合物通过结合肌动蛋白丝的尖端或与现有丝状物的侧面结合来促进肌动蛋白成核，并使生长朝向带刺的方向。此外，Arp2/3复合物还介导由Rho家族小GTP酶调节的肌动蛋白细胞骨架的外生。在响应与GTP结合的Cdc42时，Arp2/3复合物与Cdc42的底物（即WASP蛋白）结合，并启动板状伪足和丝状伪足的形成。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Arp2 抗体 (E-12) 参考文献:

  1. 表皮生长因子刺激后 Arp2/3 复合物与癌细胞前缘肌动蛋白丝倒钩末端的关系  |  Bailly, M., et al. 1999. J Cell Biol. 145: 331-45. PMID: 10209028
  2. Arp2/3复合物和肌动蛋白解聚因子/纤丝蛋白在树突组织和薄壁细胞中的肌动蛋白丝阵列中的作用  |  Svitkina, TM. and Borisy, GG. 1999. J Cell Biol. 145: 1009-26. PMID: 10352018
  3. 志贺氏菌 IcsA 蛋白激活 CDC42 效应器 N-WASP，促进 Arp2/3 复合物的肌动蛋白成核和细菌基于肌动蛋白的运动。  |  Egile, C., et al. 1999. J Cell Biol. 146: 1319-32. PMID: 10491394
  4. Arp2/3 复合物和 WASp/Scar 蛋白对肌动蛋白聚合的调节。  |  Higgs, HN. and Pollard, TD. 1999. J Biol Chem. 274: 32531-4. PMID: 10551802
  5. 控制肌动蛋白在丝端组装和分解  |  Cooper, JA. and Schafer, DA. 2000. Curr Opin Cell Biol. 12: 97-103. PMID: 10679358
  6. GRB2 通过增强神经维斯科特-阿尔德里奇综合征蛋白（N-WASp）与肌动蛋白相关蛋白（ARP2/3）复合物的相互作用，将信号传导与肌动蛋白组装联系起来。  |  Carlier, MF., et al. 2000. J Biol Chem. 275: 21946-52. PMID: 10781580
  7. Arp2/3 复合物与肌动蛋白的相互作用：成核, 高亲和力尖端封盖和形成细丝分支网络。  |  Mullins, RD., et al. 1998. Proc Natl Acad Sci U S A. 95: 6181-6. PMID: 9600938

订购信息

产品名称产品编号规格价格数量收藏夹

Arp2 抗体 (E-12)

sc-166103
200 µg/ml
$316.00

Arp2 (E-12): m-IgG Fc BP-HRP 套装

sc-528906
200 µg Ab; 10 µg BP
$354.00

Arp2 (E-12): m-IgGκ BP-HRP 套装

sc-521508
200 µg Ab, 40 µg BP
$354.00

Arp2 (E-12): m-IgG1 BP-HRP 套装

sc-543202
200 µg Ab; 20 µg BP
$354.00

Arp2 抗体 (E-12) AC

sc-166103 AC
500 µg/ml, 25% agarose
$416.00

Arp2 抗体 (E-12) HRP

sc-166103 HRP
200 µg/ml
$316.00

Arp2 抗体 (E-12) FITC

sc-166103 FITC
200 µg/ml
$330.00

Arp2 抗体 (E-12) PE

sc-166103 PE
200 µg/ml
$343.00

Arp2 抗体 (E-12) Alexa Fluor® 488

sc-166103 AF488
200 µg/ml
$357.00

Arp2 抗体 (E-12) Alexa Fluor® 546

sc-166103 AF546
200 µg/ml
$357.00

Arp2 抗体 (E-12) Alexa Fluor® 594

sc-166103 AF594
200 µg/ml
$357.00

Arp2 抗体 (E-12) Alexa Fluor® 647

sc-166103 AF647
200 µg/ml
$357.00

Arp2 抗体 (E-12) Alexa Fluor® 680

sc-166103 AF680
200 µg/ml
$357.00

Arp2 抗体 (E-12) Alexa Fluor® 790

sc-166103 AF790
200 µg/ml
$357.00

savez-vous si votre anticorps fonctionne en IF en HUVEC? 

Asked by: Sarah
Merci pour votre question. Cet anticorps convient à une utilisation IF sur des cellules humaines. Thank you for your question. This antibody is suitable for IF use on human cells.
Answered by: Tech Support Europe
Date published: 2025-01-09

I am using Arp2 (E-12): sc-166103 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great antibody for IF!Works really well in IF. We used it in human cells 1:100 dilution
Date published: 2018-03-12
Rated 5 out of 5 by from Specific bandsSpecific bands detected at 43kda by Western Blot, the sample human liver tissue extract loading 60ug,The signal is very strong with no background or non-specific bands.
Date published: 2017-08-26
Rated 4 out of 5 by from Works well on westerns, although not for IF or IPThe Arp2 antibody (E-12) detected the expected ~45Kda band and a much weaker ~70kDa band on westerns with total lysates of human tissue culture cells. There was no specific signal in the IF with mouse or human cells and the antibody also did not work for IP from cytoplasmic lysates of human tissue culture cells
Date published: 2017-07-30
Rated 5 out of 5 by from highly recommendedVery strong signal at a 1:500 dilution in western blot, highly recommended, can be used in IP and IHC
Date published: 2017-06-01
Rated 5 out of 5 by from specific signal seen.I used it detect in human astrocytes. Very strong and specific signal in the right size. Highly recommend!
Date published: 2017-05-28
Rated 4 out of 5 by from No cross species reactivity against S. cerevisiaeWe tested Antibody Arp2 (E-12) against S. cerevisiae Arp2 subunit as shown in the picture. Bovine Arp2/3 complex was loaded as a positive control and Arp2 was very well blotted. Same amount of budding yeast (S. cerevisiae) Arp2/3 complex was loaded, but Arp2 was not detected. Alternatively, Arp3 may be weakly detected (1:500 dilution, when used for the 2nd time, the signal was too low to be seen). Cell lysate of budding yeast was tested as well and nothing was detected. (sc: budding yeast Arp2/3 complex; cl: cell lysate of budding yeast; bt: bovine Arp2/3 complex).
Date published: 2017-05-01
Rated 3 out of 5 by from OK to use if Arp2 is abundantly expressedI received a free sample about two months ago as a replacement of a rabbit polyclonal that was discontinued. Among the many anti-Arp2 antibodies I have checked E-12 was the only one yielding a single band at the appropriate size in western blotting of both mouse and human endothelial cell whole cell lysates (albeit the signal was very low in the mouse sample). However, I bought the same antibody in the largest size and this time I did not get any signal on the same amount of material transferred to PVDF-FL in the same conditions. The detection system was NIR fluorescence. I have not checked this antibody with chemiluminescence.
Date published: 2017-03-04
Rated 5 out of 5 by from This antibody works well.Produced superb immunofluorescence nuclear and cytoplasmic staining in formalin-fixed HepG2 cells,so I decide to buy another one.
Date published: 2017-02-08
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Arp2 抗体 (E-12) is rated 4.6 out of 5 by 11.
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