Date published: 2025-9-9

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Arnt 1 抗体 (H-10): sc-55526

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说明书
  • Arnt 1抗体(H-10)是小鼠单克隆IgG1 κ, 在22篇文献中引用,规格为200 µg/ml
  • 免疫human物种的Arnt 1的氨基酸520-692
  • 抗-Arnt 1 抗体 (H-10) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Arnt 1
  • 抗Arnt 1抗体(H-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Arnt 1 (H-10): sc-55526免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是Arnt 1 Antibody (H-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Arnt 1 Antibody (H-10) 打包提供(请参阅下面的订购信息)。

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関連項目

Arnt 1 抗体 (H-10) 是一种小鼠单克隆 IgG1 kappa 轻链抗体,可通过免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF) 和酶联免疫吸附 (ELISA) 等应用检测小鼠、大鼠和人体样本中的 Arnt 1。抗 Arnt 1 抗体 (H-10) 有非共轭型和多种共轭型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。Arnt 1 又称芳基烃受体核转运体,是芳基烃受体(AhR)功能的重要组成部分,在细胞信号传导中起着至关重要的作用。Arnt 1 与 AhR 之间的相互作用使 AhR 发生核转位,从而与被称为 "异生物反应元件(XRE)"的特定 DNA 序列结合,激活 CYP1A1、谷胱甘肽 S 转移酶 Ya 亚基和 DT-二磷酸酶等基因的转录。Arnt 1 的结构完整性包括一个基本螺旋-环-螺旋结构域和两个 PAS 结构域,这对于与 AhR 形成异源二聚体并促进对环境毒素的转录反应至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Arnt 1 抗体 (H-10) 参考文献:

  1. 葡萄糖和内质网钙通道通过胰岛β细胞中的 presenilin 调节 HIF-1beta  |  Dror, V., et al. 2008. J Biol Chem. 283: 9909-16. PMID: 18174159
  2. ERα和 ARNT1 AF2 结构域与 SRC1 异构体 SRC1e 第 21 号外显子的结合对于雌激素和二恶英相关转录至关重要。  |  Endler, A., et al. 2012. J Cell Sci. 125: 2004-16. PMID: 22328528
  3. MicroRNA 107 可通过 HIF-1β 部分抑制内皮祖细胞的分化。  |  Meng, S., et al. 2012. PLoS One. 7: e40323. PMID: 22792280
  4. 芳基烃受体核易位体(ARNT/HIF-1β)受缺氧和模拟缺氧物质的影响。  |  Wolff, M., et al. 2013. Cell Physiol Biochem. 32: 849-58. PMID: 24081025
  5. AhR/ARNT1 的共激活因子招募。  |  Endler, A., et al. 2014. Int J Mol Sci. 15: 11100-10. PMID: 24950180
  6. ARNT/HIF-1β将高危的1q21基因增殖和微环境缺氧与多发性骨髓瘤的耐药性和不良预后联系起来。  |  Wu, C., et al. 2018. Cancer Med. 7: 3899-3911. PMID: 29926531
  7. 大西洋大黄鱼中两个 ARNT(ARNT-1 和 ARNT-2)基因的分子克隆和特征及其在缺氧和 PCB77 共同暴露时的表达。  |  Rahman, MS. and Thomas, P. 2019. Environ Toxicol. 34: 160-171. PMID: 30334616
  8. HIF-1β 通过直接控制 TRAF6 积极调节 NF-κB 活性  |  D'Ignazio, L., et al. 2020. Int J Mol Sci. 21: PMID: 32344511
  9. 由 ARNT/HIF-1β 介导的 AhR 和 HIF-1 信号通路之间的相互作用。  |  Zhang, M., et al. 2022. BMC Pharmacol Toxicol. 23: 26. PMID: 35473600
  10. DDX5 通过促进 HIF-1α 与 HIF-1β 的相互作用,并将由此产生的异源二聚体招募到其目的基因位点,从而增强 HIF-1 的活性。  |  Shirai, Y., et al. 2024. Biol Cell. 116: e2300077. PMID: 38031929

订购信息

产品名称产品编号规格价格数量收藏夹

Arnt 1 抗体 (H-10)

sc-55526
200 µg/ml
RMB2377.00

Arnt 1 (H-10): m-IgG Fc BP-HRP 套装

sc-528594
200 µg Ab; 10 µg BP
RMB2662.00

Arnt 1 (H-10): m-IgGκ BP-HRP 套装

sc-521008
200 µg Ab, 40 µg BP
RMB2662.00

Arnt 1 (H-10): m-IgG1 BP-HRP 套装

sc-543015
200 µg Ab; 20 µg BP
RMB2662.00

Arnt 1 抗体 (H-10) AC

sc-55526 AC
500 µg/ml, 25% agarose
RMB3129.00

Arnt 1 抗体 (H-10) HRP

sc-55526 HRP
200 µg/ml
RMB2377.00

Arnt 1 抗体 (H-10) FITC

sc-55526 FITC
200 µg/ml
RMB2482.00

Arnt 1 抗体 (H-10) PE

sc-55526 PE
200 µg/ml
RMB2580.00

Arnt 1 抗体 (H-10) Alexa Fluor® 488

sc-55526 AF488
200 µg/ml
RMB2685.00

Arnt 1 抗体 (H-10) Alexa Fluor® 546

sc-55526 AF546
200 µg/ml
RMB2685.00

Arnt 1 抗体 (H-10) Alexa Fluor® 594

sc-55526 AF594
200 µg/ml
RMB2685.00

Arnt 1 抗体 (H-10) Alexa Fluor® 647

sc-55526 AF647
200 µg/ml
RMB2685.00

Arnt 1 抗体 (H-10) Alexa Fluor® 680

sc-55526 AF680
200 µg/ml
RMB2685.00

Arnt 1 抗体 (H-10) Alexa Fluor® 790

sc-55526 AF790
200 µg/ml
RMB2685.00

What is the recommended secondary for use with this primary antibody?

Asked by: AbPolly
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service
Date published: 2017-01-27
  • y_2025, m_9, d_7, h_4CST
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Rated 5 out of 5 by from Produced publishable WB data on A549 and human ccRStrong bands in WB and distinct staining in IF makes this Arnt 1 antibody highly recommended. Also a handfull of citations in the primary literature are available.
Date published: 2016-12-29
Rated 5 out of 5 by from Worked great!I tested this antibody for immunoprecipitation exp. It worked very well with cell lysates so far.
Date published: 2016-12-07
Rated 4 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB -SCBT QC
Date published: 2015-05-25
Rated 4 out of 5 by from Band seen in WB using KBand seen in WB using K-562 nuclear extract. -SCBT QC
Date published: 2015-02-01
Rated 5 out of 5 by from Great immunofluorescence nuclear stainingGreat immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-27
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Arnt 1 抗体 (H-10) is rated 4.6 out of 5 by 5.
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