Date published: 2025-9-8

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Arnt 1 抗体 (A-3): sc-17811

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说明书
  • Arnt 1 抗体 A-3 是小鼠单克隆 IgG1 κ,Arnt 1抗体, 在27篇文献中引用,规格为200 µg/ml
  • 免疫human来源Arnt 1近C-terminus的520-692氨基酸
  • Arnt 1 抗体 (A-3) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 Arnt 1
  • 抗Arnt 1抗体(A-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-17811 X, 200 µg/0.1 ml)
  • 关于如何获取Arnt 1 (A-3): sc-17811免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 Arnt 1 抗体 (A-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Arnt 1 抗体 (A-3) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Arnt 1 抗体(A-3)是一种小鼠单克隆 IgG1 kappa 轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学检测小鼠、大鼠和人类的 Arnt 1 蛋白。Anti-Arnt 1 抗体(A-3)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种 Alexa Fluor® 结合物。Arnt 1,又称HIF1B、TANGO、bHLHe2、HIF1BETA或HIF-1beta,是一种重要的核蛋白,由789个氨基酸组成,在细胞对缺氧的反应中起着重要作用。Arnt 1包含一个碱性螺旋-环-螺旋(bHLH)结构域、一个PAC(PAS相关C末端)结构域和两个PAS(PER-ARNT-SIM)结构域,这些结构域对于转录因子的功能至关重要。Arnt 1对于与芳香烃受体(AhR)的二聚化至关重要,它促进受体核转运,并使其与称为异生物反应元件(XRE)的特定DNA序列结合。这种相互作用调节参与解毒过程的基因,如CYP1A1和谷胱甘肽S-转移酶,从而影响人体对环境毒素的反应并保持细胞内环境稳定。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Arnt 1 抗体 (A-3) 参考文献:

  1. 确定 Ah 受体核转运蛋白(Arnt)是 Ah 受体 DNA 结合形式的组成部分。  |  Reyes, H., et al. 1992. Science. 256: 1193-5. PMID: 1317062
  2. ERα和 ARNT1 AF2 结构域与 SRC1 异构体 SRC1e 第 21 号外显子的结合对于雌激素和二恶英相关转录至关重要。  |  Endler, A., et al. 2012. J Cell Sci. 125: 2004-16. PMID: 22328528
  3. 芳基烃受体核易位体(ARNT/HIF-1β)受缺氧和模拟缺氧物质的影响。  |  Wolff, M., et al. 2013. Cell Physiol Biochem. 32: 849-58. PMID: 24081025
  4. AhR/ARNT1 的共激活因子招募。  |  Endler, A., et al. 2014. Int J Mol Sci. 15: 11100-10. PMID: 24950180
  5. ARNT/HIF-1β将高危的1q21基因增殖和微环境缺氧与多发性骨髓瘤的耐药性和不良预后联系起来。  |  Wu, C., et al. 2018. Cancer Med. 7: 3899-3911. PMID: 29926531
  6. 由 ARNT/HIF-1β 介导的 AhR 和 HIF-1 信号通路之间的相互作用。  |  Zhang, M., et al. 2022. BMC Pharmacol Toxicol. 23: 26. PMID: 35473600
  7. Ah 受体和 Ah 受体核转运体的转录激活结构域。  |  Sogawa, K., et al. 1995. J Cancer Res Clin Oncol. 121: 612-20. PMID: 7559746
  8. 一种新型碱性螺旋-环-螺旋/PAS因子(Arnt2)的cDNA克隆和组织特异性表达,其序列与芳基烃受体核转运体(Arnt)非常相似。  |  Hirose, K., et al. 1996. Mol Cell Biol. 16: 1706-13. PMID: 8657146
  9. 克隆与 Ah 受体核转运体(ARNT)同源的 ARNT2 并在大脑和肾脏中选择性表达。  |  Drutel, G., et al. 1996. Biochem Biophys Res Commun. 225: 333-9. PMID: 8753765
  10. 新型碱性螺旋-环-螺旋/PAS 蛋白(BMAL1)的 cDNA 克隆和组织特异性表达,以及具有替代翻译起始位点用法的替代剪接变体的鉴定。  |  Ikeda, M. and Nomura, M. 1997. Biochem Biophys Res Commun. 233: 258-64. PMID: 9144434
  11. Ah 受体,一种新型配体激活转录因子。  |  Sogawa, K. and Fujii-Kuriyama, Y. 1997. J Biochem. 122: 1075-9. PMID: 9498548
  12. 哺乳动物时钟基因 Clock 的伴侣 BMAL1 在大鼠蛛网膜上核的昼夜节律摆动  |  Honma, S., et al. 1998. Biochem Biophys Res Commun. 250: 83-7. PMID: 9735336

订购信息

产品名称产品编号规格价格数量收藏夹

Arnt 1 抗体 (A-3)

sc-17811
200 µg/ml
RMB2377.00

Arnt 1 (A-3): m-IgG Fc BP-HRP 套装

sc-528323
200 µg Ab; 10 µg BP
RMB2662.00

Arnt 1 (A-3): m-IgGκ BP-HRP 套装

sc-520669
200 µg Ab, 40 µg BP
RMB2662.00

Arnt 1 (A-3): m-IgG1 BP-HRP 套装

sc-542866
200 µg Ab; 20 µg BP
RMB2662.00

Arnt 1 抗体 (A-3) X

sc-17811 X
200 µg/0.1 ml
RMB2377.00

Arnt 1 抗体 (A-3) AC

sc-17811 AC
500 µg/ml, 25% agarose
RMB3129.00

Arnt 1 抗体 (A-3) HRP

sc-17811 HRP
200 µg/ml
RMB2377.00

Arnt 1 抗体 (A-3) FITC

sc-17811 FITC
200 µg/ml
RMB2482.00

Arnt 1 抗体 (A-3) PE

sc-17811 PE
200 µg/ml
RMB2580.00

Arnt 1 抗体 (A-3) Alexa Fluor® 488

sc-17811 AF488
200 µg/ml
RMB2685.00

Arnt 1 抗体 (A-3) Alexa Fluor® 546

sc-17811 AF546
200 µg/ml
RMB2685.00

Arnt 1 抗体 (A-3) Alexa Fluor® 594

sc-17811 AF594
200 µg/ml
RMB2685.00

Arnt 1 抗体 (A-3) Alexa Fluor® 647

sc-17811 AF647
200 µg/ml
RMB2685.00

Arnt 1 抗体 (A-3) Alexa Fluor® 680

sc-17811 AF680
200 µg/ml
RMB2685.00

Arnt 1 抗体 (A-3) Alexa Fluor® 790

sc-17811 AF790
200 µg/ml
RMB2685.00

When should the TransCruz reagent sc-17811 X be used instead of the standard form sc-17811?

Asked by: Germaine
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great for WB. IF. ChIP-qPCR2ug of this antibody used for blotting, it detectes one band at the expected molecular weight. It works very fine in Immunofluoresence assay in cancer cell lines. It also works (4ug) in ChIP-qPCR
Date published: 2020-02-17
Rated 4 out of 5 by from GreatNice band seen in western blotting using 293T whole cell lysate .
Date published: 2017-10-11
Rated 5 out of 5 by from Versatile antibodyArnt 1 Antibody (A-3) produced strong results by Western blot, IHCP and IF
Date published: 2017-01-27
Rated 4 out of 5 by from Band seen in WB using HeLa whole cell lysateBand seen in WB using HeLa whole cell lysate. -SCBT QC
Date published: 2015-05-26
Rated 5 out of 5 by from Produced excellent immunofluorescence nuclearProduced excellent immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-09
Rated 4 out of 5 by from Nice band detected in WB using KNice band detected in WB using K-562 whole cell lysate. -SCBT QC
Date published: 2015-02-24
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Arnt 1 抗体 (A-3) is rated 4.5 out of 5 by 6.
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