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Aquaporin 2/AQP2 Antibody (E-2) - Immunohistochemistry - Image 368887
AQP2 抗体 (E-2): sc-515770. 福尔马林固定, 石蜡包埋的大鼠肾组织的免疫过氧化物酶染色, 显示肾小球和肾小管细胞的膜和细胞质染色.
AQP2 抗体 (E-2): sc-515770. 福尔马林固定, 石蜡包埋的大鼠肾组织的免疫过氧化物酶染色, 显示肾小球和肾小管细胞的膜和细胞质染色.

Aquaporin 2/AQP2 抗体 (E-2): sc-515770

4.7(6)
寫評論提問

说明书
  • Aquaporin 2/AQP2 抗体 E-2 是小鼠单克隆 IgG1 κ，Aquaporin 2/AQP2抗体, 在68篇文献中引用，规格为200 µg/ml
  • 特异性表位图谱分析在human的AQP2的C-terminal细胞质域内的232-271氨基酸之间进行
  • Aquaporin 2/AQP2 抗体 (E-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 AQP2
  • 抗Aquaporin 2/AQP2抗体（E-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Aquaporin 2/AQP2 (E-2): sc-515770的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Aquaporin 2/AQP2 抗体 (E-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Aquaporin 2/AQP2 抗体 (E-2) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 AQP 抗体 包括 AQP0, AQP1, AQP2, AQP3, AQP4, AQP5, AQP7, AQP8和AQP9

AQP2抗体（E-2）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类的AQP2。抗AQP2抗体（E-2）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。AQP2在调节体内水平衡方面发挥着至关重要的作用，特别是通过促进肾集合管对血管加压素的响应，从而促进水的重吸收。这一功能对于维持体内平衡至关重要，特别是在脱水或血浆渗透压增加的情况下。AQP2在水通道蛋白中是独一无二的，因为它是唯一直接受血管加压素调节的水通道，可增强活性并促进尿液浓缩。AQP2的正常运作对于预防尿崩症等疾病至关重要，尿崩症是指身体无法浓缩尿液，从而导致尿频和口渴。了解AQP2的调节对于制定肾脏相关疾病的治疗策略以及管理各种临床环境下的体液平衡非常重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Aquaporin 2/AQP2 抗体 (E-2) 参考文献:

  1. 肌动蛋白直接与不同的膜通道蛋白相互作用，并影响通道活性：以AQP2为模型。  |  Sasaki, S., et al. 2014. Biochim Biophys Acta. 1838: 514-20. PMID: 23770358
  2. 集水管中 AQP2 的调控：强调血管紧张素 II 或醛固酮的作用  |  Lee, BH. and Kwon, TH. 2007. Electrolyte Blood Press. 5: 15-22. PMID: 24459495
  3. E3 连接酶 CHIP 通过 HSP70 调节 AQP2 的丰度  |  Centrone, M., et al. 2017. Cell Physiol Biochem. 44: 515-531. PMID: 29145196
  4. 人类AQP2的磷酸化及其在转运中的作用。  |  Törnroth-Horsefield, S. 2020. Vitam Horm. 112: 95-117. PMID: 32061351
  5. AQP2：与先天性肾源性尿崩症相关的突变以及翻译后修饰和蛋白质-蛋白质相互作用调节。  |  Gao, C., et al. 2020. Cells. 9: PMID: 32993088
  6. Aqp2+祖细胞维护和修复远端肾节  |  Gao, C., et al. 2022. J Am Soc Nephrol. 33: 1357-1376. PMID: 35318267
  7. AQP2通过调节脑出血后的TLR4/NFκB-p65通路促进星形胶质细胞活化  |  Deng, S., et al. 2022. Front Immunol. 13: 847360. PMID: 35386692
  8. 使用AQP2回收行程抑制剂鉴定AQP2 S256磷酸化细胞内位点。  |  Cheung, PW., et al. 2023. Am J Physiol Renal Physiol. 324: F152-F167. PMID: 36454701
  9. 基因组工程改造的mpkCCDc14细胞作为研究AQP2的新资源。  |  Jang, HJ., et al. 2023. Int J Mol Sci. 24: PMID: 36675199
  10. TNF通过miR137依赖性途径抑制AQP2表达。  |  Hao, S., et al. 2024. Am J Physiol Renal Physiol. 326: F152-F164. PMID: 37969102

订购信息

产品名称产品编号规格价格数量收藏夹

Aquaporin 2/AQP2 抗体 (E-2)

sc-515770
200 µg/ml
RMB2377.00

Aquaporin 2/AQP2 (E-2): m-IgG Fc BP-HRP 套装

sc-528061
200 µg Ab; 10 µg BP
RMB2662.00

Aquaporin 2/AQP2 (E-2): m-IgG1 BP-HRP 套装

sc-533434
200 µg Ab; 20 µg BP
RMB2662.00

Aquaporin 2/AQP2 抗体 (E-2) AC

sc-515770 AC
500 µg/ml, 25% agarose
RMB3129.00

Aquaporin 2/AQP2 抗体 (E-2) HRP

sc-515770 HRP
200 µg/ml
RMB2377.00

Aquaporin 2/AQP2 抗体 (E-2) FITC

sc-515770 FITC
200 µg/ml
RMB2482.00

Aquaporin 2/AQP2 抗体 (E-2) PE

sc-515770 PE
200 µg/ml
RMB2580.00

Aquaporin 2/AQP2 抗体 (E-2) Alexa Fluor® 488

sc-515770 AF488
200 µg/ml
RMB2685.00

Aquaporin 2/AQP2 抗体 (E-2) Alexa Fluor® 546

sc-515770 AF546
200 µg/ml
RMB2685.00

Aquaporin 2/AQP2 抗体 (E-2) Alexa Fluor® 594

sc-515770 AF594
200 µg/ml
RMB2685.00

Aquaporin 2/AQP2 抗体 (E-2) Alexa Fluor® 647

sc-515770 AF647
200 µg/ml
RMB2685.00

Aquaporin 2/AQP2 抗体 (E-2) Alexa Fluor® 680

sc-515770 AF680
200 µg/ml
RMB2685.00

Aquaporin 2/AQP2 抗体 (E-2) Alexa Fluor® 790

sc-515770 AF790
200 µg/ml
RMB2685.00

Hello, what is the recommended IHC protocol for this antibody? I would particular like to know about antigen retrieval, blocking and primary antibody incubation time/dilution.

Asked by: saravetvet
Thank you for your question. You can find our recommended IHC protocol under this link: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining. We recommend antigen retrieval using citrate buffer (also described in the protocol) and our plant-based UltraCruz® Blocking Reagent: sc-516214 for blocking. You can find recommended antibody dilutions in the product datasheet. Please get in touch with our technical service if you require further information.
Answered by: Tech Support Europe
Date published: 2019-05-28

How can AQP2 (E-2): sc-515770 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenniferc
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to HRP (sc-515770 HRP), phycoerythrin (sc-515770 PE), fluoroscein (sc-515770 FITC) and AlexaFluors.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from very clear image of IFIt works very well for immunofluorescence staining.
Date published: 2022-10-21
Rated 5 out of 5 by from 功能很出色用的真好，条带跟ihc做出来特别好看，用的真好，条带跟ihc做出来特别好看，用的真好，条带跟ihc做出来特别好看，
Date published: 2021-08-02
Rated 5 out of 5 by from Work in kidney paraffin sectionsI tried this antibody on human baby (6 weeks) kidney paraffin sections for 1:25 dilution and it worked nicely. It was antigen retrieved with heat mediated antigen retrieval in 1mM EDTA buffer pH 8.0. It labeled the principal cells in the collecting duct as reported in literature.
Date published: 2019-04-10
Rated 4 out of 5 by from Good antibodyI used this antibody for cell IF, really good images.
Date published: 2017-11-28
Rated 5 out of 5 by from Works great on paraffin section immunostainingI tried this antibody on embryonic day E16.5 mouse kidney paraffin sections for 1:200 dilution and it worked nicely. The kidney was fixed in 4% PFA for 3 hours and sectioned at 10um thickness. It was antigen retrieved as well. It labeled the principal cells in the collecting duct as reported in literature.
Date published: 2017-08-24
Rated 4 out of 5 by from Suitable for feline kidney cellsThe monoclonal AQP2 Antibody (E-2, sc-515770) is suitable for staining of primary feline kidney cells in immunofluorescence application.
Date published: 2017-04-12
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Aquaporin 2/AQP2 抗体 (E-2) is rated 4.7 out of 5 by 6.
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