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Annexin-2/ANXA2 抗体 (H-5): sc-48397

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说明书
  • Annexin-2/ANXA2抗体(H-5)是小鼠单克隆IgG1 κ, 在4篇文献中引用,规格为200 µg/ml
  • 免疫human物种的Annexin II的氨基酸1-50
  • 推荐用于 mouse, rat 和 human 来源的Annexin II WB, IP, IF, IHC(P) 和 ELISA检测; 也和以下物种反应,包括: 和 canine and bovine
  • 看 Annexin-2/ANXA2 (C-10): sc-28385,可查看有标记的 Annexin-2/ANXA2 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP和 m-IgG1 BP-HRP是Annexin-2/ANXA2 Antibody (H-5) for WB and IHC(P) applications. 的首选辅助检测试剂,这些试剂现与Annexin-2/ANXA2 Antibody (H-5) 打包提供(请参阅下面的订购信息)。
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関連項目

Annexin II 抗体(H-5)是针对人 Annexin II(又称 Annexin A2、Calpactin I 重链、Chromobindin-8、Lipocortin II、p36 和 Placental Anticoagulant Protein IV)1-50 氨基酸的小鼠单克隆 IgG1 kappa 轻链抗体。附件蛋白 II 是一种钙依赖性磷脂结合蛋白,其特征是有一个由四个附件蛋白重复序列组成的保守核心结构域,每个重复序列形成一个螺旋-环-螺旋结构,能以钙依赖性方式与磷脂结合。这一结构特征有助于内吞、外吞和细胞粘附等与膜相关的过程。Annexin II 独特的 N 端结构域允许它与其他蛋白质相互作用,特别是与 S100A10(p11)(即钙粘蛋白 I)形成异构四聚体复合物,后者在信号转导和细胞骨架组织中发挥着重要作用。通过了解附件蛋白 II 的结构,研究人员可以更好地理解其参与膜贩运和调节纤维蛋白溶解等关键细胞功能的情况。抗附件蛋白 II 抗体 (H-5) 可通过免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF)、石蜡包埋切片免疫组织化学 (IHCP) 和酶联免疫吸附试验 (ELISA) 检测小鼠、大鼠和人源的附件蛋白 II,还可对犬和牛等其他物种产生反应。利用 Annexin II 单克隆抗体 (H-5) 靶向 Annexin II,研究人员可以研究它在生理过程和病理状态(如癌症进展、炎症和凝血障碍)中的作用。Annexin II (H-5) 抗体既有非共轭型,也有多种共轭型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和各种 Alexa Fluor® 共轭物。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Annexin-2/ANXA2 抗体 (H-5) 参考文献:

  1. Annexin II的过度表达与基质粘蛋白-C的过度表达相关:结直肠癌的预后标志物。  |  Emoto, K., et al. 2001. Cancer. 92: 1419-26. PMID: 11745218
  2. 在前列腺癌细胞中,Annexin II的表达减少或消失,其重新表达抑制前列腺癌细胞的迁移。  |  Liu, JW., et al. 2003. Oncogene. 22: 1475-85. PMID: 12629510
  3. 在发育中的鸟类心脏上皮-间质转化过程中,Annexin II介导的纤溶酶原激活TGF-beta3。  |  Krishnan, S., et al. 2004. Dev Biol. 265: 140-54. PMID: 14697359
  4. 抗annexin II抗体在系统性自身免疫性疾病和抗磷脂综合征中的作用。  |  Salle, V., et al. 2008. J Clin Immunol. 28: 291-7. PMID: 18322784
  5. Annexin II/annexin II受体轴调节前列腺癌的粘附、迁移、归巢和生长。  |  Shiozawa, Y., et al. 2008. J Cell Biochem. 105: 370-80. PMID: 18636554
  6. 通过其与HAb18G/CD147的相互作用,Annexin II促进人肝癌细胞在体外侵袭和迁移。  |  Zhao, P., et al. 2010. Cancer Sci. 101: 387-95. PMID: 20047591
  7. 过氧化氢引起的Annexin II依赖性肌动蛋白重塑需要金属蛋白酶/鞘脂途径。  |  Cinq-Frais, C., et al. 2015. Redox Biol. 4: 169-79. PMID: 25574848
  8. Tenascin-c 通过附件蛋白 II 在巨噬细胞中产生促血管生成表型。  |  Wang, Z., et al. 2018. J Cell Mol Med. 22: 429-438. PMID: 28857429
  9. 在胃癌中表达Annexin II及其在胃癌转移中的作用。  |  Han, F., et al. 2017. World J Gastroenterol. 23: 7009-7015. PMID: 29097873
  10. Annexin A2和癌症:系统综述。  |  Christensen, MV., et al. 2018. Int J Oncol. 52: 5-18. PMID: 29115416

订购信息

产品名称产品编号规格价格数量收藏夹

Annexin-2/ANXA2 抗体 (H-5)

sc-48397
200 µg/ml
CNY2422.00

Annexin-2/ANXA2 (H-5): m-IgG Fc BP-HRP 套装

sc-538939
200 µg Ab; 10 µg BP
CNY2715.00

Annexin-2/ANXA2 (H-5): m-IgG1 BP-HRP 套装

sc-541167
200 µg Ab; 20 µg BP
CNY2715.00

I am using Annexin II (H-5): sc-48397 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by caihongbo from IHC analysisIHC analysis Annexin II expression in human colon cancer tissues . The results showed that the target protein was highly expressed in the cell membrane and cytoplasm of colon cancer tissues.We use dilution 1:300 ,it's very easy got the successful result.
Date published: 2017-09-09
Rated 5 out of 5 by moon098 from strong bandI got strong band in WB in human kidney tissue extracts .I believe it's a good choice to bought this antibody than other suppliers.
Date published: 2017-09-03
Rated 5 out of 5 by caijin from specific bandspecific band in WB in human kidney tumor extracts .It's a good choice to detect Annexin II.
Date published: 2017-06-03
Rated 5 out of 5 by NZ from Publishable data obtained by immunocytochemistryPublishable data obtained by immunocytochemistry using rat PC12 cells and primary cortical neurons. -SCBT Publication Review
Date published: 2015-05-07
Rated 5 out of 5 by LF from Superb immunoperoxidase membrane and cytoplasmicSuperb immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human gall bladder tissue. -SCBT QC
Date published: 2015-02-22
Rated 5 out of 5 by MC from Endogenous levels of Annexin II detectedEndogenous levels of Annexin II detected by WB using 293T cells. -SCBT QC
Date published: 2015-02-11
Rated 5 out of 5 by TO from Positive detection of the Annexin II proteinPositive detection of the Annexin II protein by WB using various cell lines: HeLa, K562, MDCK, NIH/3T3 and KNRK cells. -SCBT QC
Date published: 2015-01-09
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Annexin-2/ANXA2 抗体 (H-5) is rated 5.0 out of 5 by 7.
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