Date published: 2025-9-7
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Annexin-2/ANXA2 Antibody (C-10) - Western Blotting - Image 375266
Annexin II 抗体 (C-10): sc-28385. HeLa (A), K-562 (B), NIH/3T3 (C), KNRK (D), ECV304 (E) 和 A-431 (F) 全细胞裂解液中 Annexin II 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
Annexin II 抗体 (C-10): sc-28385. HeLa (A), K-562 (B), NIH/3T3 (C), KNRK (D), ECV304 (E) 和 A-431 (F) 全细胞裂解液中 Annexin II 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

Annexin-2/ANXA2 抗体 (C-10): sc-28385

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说明书
  • Annexin-2/ANXA2 抗体 C-10 是小鼠单克隆 IgG2a κ，Annexin-2/ANXA2抗体, 在80篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Annexin II的氨基酸1-50
  • Annexin-2/ANXA2 抗体 (C-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Annexin II
  • 抗Annexin-2/ANXA2抗体（C-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Annexin-2/ANXA2 (C-10): sc-28385的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 Annexin-2/ANXA2 抗体 (C-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Annexin-2/ANXA2 抗体 (C-10) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Annexin 抗体 包括 Annexin A10, Annexin A13, Annexin A9, Annexin I, Annexin II, Annexin III, Annexin IV, Annexin V, Annexin VI和Annexin VII

Annexin II 抗体（C-10）是一种小鼠单克隆 IgG2a kappa 轻链抗体，针对人类 Annexin II（也称为 Annexin A2、Calpactin-2、Chromobindin-8、Lipocortin II、p36 和 Placental Anticoagulant Protein IV）的氨基酸 1-50 进行免疫。抗Annexin II抗体（C-10）采用蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）等技术检测人、小鼠和大鼠的Annexin II蛋白。Annexin II是一种钙依赖性磷脂结合蛋白，参与多种细胞过程，如膜运输、细胞运动和细胞粘附。Annexin II在纤维蛋白溶解中起着关键作用，它充当组织纤溶酶原激活剂（tPA）和纤溶酶原的受体，促进纤溶酶原转化为纤溶酶，从而对维持正常的血液凝固和炎症反应至关重要。附件蛋白II可以以单体形式存在，也可以与S100A10（p11）形成异四聚体复合物，后者被称为钙蛋白激酶I，在各种信号传导途径中起着重要作用。在四聚体形式下，Annexin II是蛋白激酶C（PKC）和Src家族激酶的高效底物，表明其在磷酸化依赖性信号传导机制中的作用。抗Annexin II抗体（C-10）使研究人员能够研究Annexin II在病理条件下的作用，例如癌症进展、炎症和凝血障碍。Annexin II 单克隆抗体（C-10）有未结合和多种结合形式，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和各种 Alexa Fluor® 结合物，为不同的实验应用提供了灵活性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Annexin-2/ANXA2 抗体 (C-10) 参考文献:

  1. Annexin II和细胞表面纤维蛋白溶解的调节。  |  Hajjar, KA. and Acharya, SS. 2000. Ann N Y Acad Sci. 902: 265-71. PMID: 10865846
  2. Annexin-II在消化道癌症中的作用：与胃泌素/促胃泌素的相互作用。  |  Singh, P. 2007. Cancer Lett. 252: 19-35. PMID: 17188424
  3. 作为登革病毒血清型 2 交互蛋白介导病毒在 Vero 细胞上的交互作用的 Annexin II。  |  Aliyu, IA., et al. 2019. Viruses. 11: PMID: 30970587
  4. 酪氨酸磷酸化对 annexin II 四聚体的调节作用。  |  Hubaishy, I., et al. 1995. Biochemistry. 34: 14527-34. PMID: 7578058
  5. 附件蛋白超级基因家族的结构演变。  |  Smith, PD. and Moss, SE. 1994. Trends Genet. 10: 241-6. PMID: 8091504
  6. Annexin II 通过翻译后机制上调细胞中 p11 蛋白的水平。  |  Puisieux, A., et al. 1996. Biochem J. 313 (Pt 1): 51-5. PMID: 8546709
  7. S100C 与附件蛋白 I N 端结构域的钙依赖性结合。  |  Mailliard, WS., et al. 1996. J Biol Chem. 271: 719-25. PMID: 8557678
  8. Annexin II 四聚体：结构与功能。  |  Waisman, DM. 1995. Mol Cell Biochem. 149-150: 301-22. PMID: 8569746
  9. 地塞米松可诱导大鼠星形胶质细胞原代培养物中细胞内和膜相关脂皮质素-1（annexin-1）的增加。  |  McLeod, JD. and Bolton, C. 1995. Cell Mol Neurobiol. 15: 193-205. PMID: 8590451
  10. IL-1beta 对 A549 细胞中 cPLA2, COX2 和脂皮质素 1 表达的协同调控。  |  Croxtal, JD., et al. 1996. Biochem Biophys Res Commun. 220: 491-5. PMID: 8607793
  11. 外泌过程中的附蛋白 II：儿茶酚胺的分泌需要 p36 转位至绒毛膜细胞的浆膜下区域。  |  Chasserot-Golaz, S., et al. 1996. J Cell Biol. 133: 1217-36. PMID: 8682860
  12. 细胞外附件蛋白II。  |  Siever, DA. and Erickson, HP. 1997. Int J Biochem Cell Biol. 29: 1219-23. PMID: 9451817

订购信息

产品名称产品编号规格价格数量收藏夹

Annexin-2/ANXA2 抗体 (C-10)

sc-28385
200 µg/ml
RMB2377.00

Annexin-2/ANXA2 (C-10): m-IgG Fc BP-HRP 套装

sc-528442
200 µg Ab; 10 µg BP
RMB2662.00

Annexin-2/ANXA2 (C-10): m-IgGκ BP-HRP 套装

sc-520820
200 µg Ab, 40 µg BP
RMB2662.00

Annexin-2/ANXA2 (C-10): m-IgG2a BP-HRP 套装

sc-547100
200 µg Ab; 10 µg BP
RMB2662.00

Annexin-2/ANXA2 抗体 (C-10) AC

sc-28385 AC
500 µg/ml, 25% agarose
RMB3129.00

Annexin-2/ANXA2 抗体 (C-10) HRP

sc-28385 HRP
200 µg/ml
RMB2377.00

Annexin-2/ANXA2 抗体 (C-10) FITC

sc-28385 FITC
200 µg/ml
RMB2482.00

Annexin-2/ANXA2 抗体 (C-10) PE

sc-28385 PE
200 µg/ml
RMB2580.00

Annexin-2/ANXA2 抗体 (C-10) Alexa Fluor® 488

sc-28385 AF488
200 µg/ml
RMB2685.00

Annexin-2/ANXA2 抗体 (C-10) Alexa Fluor® 546

sc-28385 AF546
200 µg/ml
RMB2685.00

Annexin-2/ANXA2 抗体 (C-10) Alexa Fluor® 594

sc-28385 AF594
200 µg/ml
RMB2685.00

Annexin-2/ANXA2 抗体 (C-10) Alexa Fluor® 647

sc-28385 AF647
200 µg/ml
RMB2685.00

Annexin-2/ANXA2 抗体 (C-10) Alexa Fluor® 680

sc-28385 AF680
200 µg/ml
RMB2685.00

Annexin-2/ANXA2 抗体 (C-10) Alexa Fluor® 790

sc-28385 AF790
200 µg/ml
RMB2685.00

I will try immunohistochemistry using paraffin embedded tissue. Please tell me the suitable positive control tissue.

Asked by: A350
Thank you for your question. The official website uses human prostate tissue.Thank you.
Answered by: Jiajun Z
Date published: 2025-08-04

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 10
Date published: 2017-01-13

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue? Or even double or triple staining on the same samples?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-28385 PE), fluorescein (sc-28385 FITC), Alexa Fluor® 488 (sc-28385 AF488) or Alexa Fluor® 647 (sc-28385 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 10
Date published: 2017-01-13
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Rated 5 out of 5 by from Great featureMy colleague purchased this product and was very good
Date published: 2024-11-15
Rated 4 out of 5 by from Worked fine in WBThe sample works well with my Hek393T lisates. I saw and strong band near 37KDa. I used it ON and very concentrated but I think should be fine in a lower concentration.
Date published: 2022-05-02
Rated 5 out of 5 by from Annexin II Antibody (C-10) Western blotworks well for western blot analysis of human salivary gland tissues
Date published: 2021-02-13
Rated 5 out of 5 by from Very good efficiency for Western blottingVery good signal for Annexin A2 revealed by this antibody by Western blotting. Here it is shown a representative image of total protein lysate of HUVEC cells (sample only 5ʏ).
Date published: 2019-07-19
Rated 5 out of 5 by from Great Antibody, Very Strong Signal!!We used this antibody for Western blot analysis (1:800) and got very strong signal and was able to reuse antibody multiple times.
Date published: 2019-01-31
Rated 5 out of 5 by from Powerful AntibodyThis antibody has a good, specific signal for both IF, IP, and Westerns. For Westerns, we've been able to reuse a 1:1000 dilution of the antibody several times before needing to spike with fresh stock. It's great!
Date published: 2018-11-20
Rated 5 out of 5 by from excellent！The bands is very clear and the background is very clean
Date published: 2018-10-12
Rated 5 out of 5 by from Use this antibody on broilersI applied this antibody to exosomes isolated from broiler plasma, and the size of the bands was consistent with the instructions.
Date published: 2017-11-24
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Annexin-2/ANXA2 抗体 (C-10) is rated 4.8 out of 5 by 28.
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