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Annexin-2/ANXA2 抗体 (3D5): sc-47696

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说明书
  • Annexin-2/ANXA2抗体(3D5)是小鼠单克隆IgG2a κ, 在16篇文献中引用,规格为200 µg/ml
  • 针对human骨组织中的全天然附件蛋白
  • 抗-Annexin-2/ANXA2 抗体 (3D5) 推荐用于 WB, IP 和 IF,检测mouse, rat 和human 来源的 Annexin II
  • 抗Annexin-2/ANXA2抗体(3D5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP是Annexin-2/ANXA2 Antibody (3D5) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与Annexin-2/ANXA2 Antibody (3D5) 搭配使用(请参阅下面的订购信息)。
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関連項目

Annexin II抗体(3D5)是一种小鼠单克隆IgG2a kappa轻链抗体,针对人类骨骼组织中的天然Annexin蛋白,能够通过蛋白质印迹(WB)、免疫沉淀(IP)和免疫荧光(IF)检测小鼠、大鼠和人类来源的Annexin II。Annexin II,也称为Annexin A2、Calpactin II、Chromobindin-8、Lipocortin II或p36,是一种钙依赖性磷脂结合蛋白,在各种细胞过程中发挥着关键作用。Annexin II主要位于细胞质和细胞表面,可调节与膜相关的事件,如内吞作用、外排作用和细胞粘附。表面表达的Annexin II可作为纤溶酶原和纤溶酶原激活剂(tPA)的受体,促进纤溶酶的产生,并在纤维蛋白溶解和血液凝固中发挥关键作用。Annexin II可以以单体形式存在,也可以与S100A10(p11)形成异四聚体复合物,后者被称为钙蛋白激酶I,参与信号转导通路。Annexin II也是蛋白激酶C(PKC)和Src家族激酶的底物,表明它参与了磷酸化依赖性信号传导机制。抗Annexin II抗体(3D5)使研究人员能够研究Annexin II在病理条件下的作用,例如癌症进展、炎症和凝血障碍。Annexin II (3D5) 抗体有未结合和多种结合形式,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 以及各种 Alexa Fluor® 结合物,为不同的实验应用提供了多功能性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Annexin-2/ANXA2 抗体 (3D5) 参考文献:

  1. 急性早幼粒细胞白血病中的Annexin II和出血。  |  Menell, JS., et al. 1999. N Engl J Med. 340: 994-1004. PMID: 10099141
  2. Annexin II与可能构成独特亚群的mRNA相关。  |  Vedeler, A. and Hollås, H. 2000. Biochem J. 348 Pt 3: 565-72. PMID: 10839987
  3. Annexin II介导人单核细胞对纤溶酶原依赖性基质的侵袭:巨噬细胞增强表达。  |  Brownstein, C., et al. 2004. Blood. 103: 317-24. PMID: 14504107
  4. Annexin II调节体内纤维蛋白原稳态和新生血管生成。  |  Ling, Q., et al. 2004. J Clin Invest. 113: 38-48. PMID: 14702107
  5. Annexin II刺激RANKL表达通过MAPK。  |  Li, F., et al. 2005. J Bone Miner Res. 20: 1161-7. PMID: 15940368
  6. Annexin II在血管生成和肿瘤进展中的作用:潜在的治疗靶点。  |  Sharma, MC. and Sharma, M. 2007. Curr Pharm Des. 13: 3568-75. PMID: 18220793
  7. Annexin II受体诱导细胞凋亡,与Annexin II无关。  |  Xiong, Y., et al. 2013. Apoptosis. 18: 925-39. PMID: 23640736
  8. Annexin II包含两种类型的Ca(2+)-结合位点。  |  Jost, M., et al. 1994. Biochem J. 298 Pt 3: 553-9. PMID: 7908188
  9. 在大鼠主动脉平滑肌中,Annexin II抑制G蛋白调节的三磷酸肌醇的形成。  |  Schelling, JR., et al. 1996. Am J Physiol. 270: F682-90. PMID: 8967347
  10. Annexin II以钙依赖性和钙非依赖性的方式与A549细胞膜结合。  |  Liu, L., et al. 1997. Cell Signal. 9: 299-304. PMID: 9218131

订购信息

产品名称产品编号规格价格数量收藏夹

Annexin-2/ANXA2 抗体 (3D5)

sc-47696
200 µg/ml
CNY2422.00

Annexin-2/ANXA2 (3D5): m-IgG Fc BP-HRP 套装

sc-525551
200 µg Ab; 10 µg BP
CNY2715.00

Annexin-2/ANXA2 抗体 (3D5) AC

sc-47696 AC
500 µg/ml, 25% agarose
CNY3189.00

Annexin-2/ANXA2 抗体 (3D5) HRP

sc-47696 HRP
200 µg/ml
CNY2422.00

Annexin-2/ANXA2 抗体 (3D5) FITC

sc-47696 FITC
200 µg/ml
CNY2527.00

Annexin-2/ANXA2 抗体 (3D5) PE

sc-47696 PE
200 µg/ml
CNY2625.00

Annexin-2/ANXA2 抗体 (3D5) Alexa Fluor® 488

sc-47696 AF488
200 µg/ml
CNY2738.00

Annexin-2/ANXA2 抗体 (3D5) Alexa Fluor® 546

sc-47696 AF546
200 µg/ml
CNY2738.00

Annexin-2/ANXA2 抗体 (3D5) Alexa Fluor® 594

sc-47696 AF594
200 µg/ml
CNY2738.00

Annexin-2/ANXA2 抗体 (3D5) Alexa Fluor® 647

sc-47696 AF647
200 µg/ml
CNY2738.00

Annexin-2/ANXA2 抗体 (3D5) Alexa Fluor® 680

sc-47696 AF680
200 µg/ml
CNY2738.00

Annexin-2/ANXA2 抗体 (3D5) Alexa Fluor® 790

sc-47696 AF790
200 µg/ml
CNY2738.00

What about cross-reactivity with annexinA1 and annexin A3?

Asked by: mtsanna
Thank you for your question. This antibody is specific for Annexin II and should not pick up Annexin 1 and Annexin III. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-11-18

What is the recommended fixation method for immunofluorescence staining with Annexin II (3D5): sc-47696 monoclonal antibody?

Asked by: TinTin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by TimS from Works GreatAll of the Santa Cruz Annexin II antibodies have been good experiences for us.
Date published: 2019-02-06
Rated 5 out of 5 by sticky from Specific staining (cell membranes)Specific staining (cell membranes). Excellent antibody for IHC. Reagents for blocking steps & seconary antibody from Dako
Date published: 2017-02-14
Rated 5 out of 5 by FL from Robust band seen in WB in KRobust band seen in WB in K-562 whole cell lysate. -SCBT QC
Date published: 2015-05-18
Rated 5 out of 5 by MS from Good specificity in WB in human Annexin IIGood specificity in WB in human Annexin II transfected 293 whole cell lysate. -SCBT QC
Date published: 2015-04-28
Rated 5 out of 5 by FL from Published data for WB and IF in HEK293T cellsPublished data for WB and IF in HEK293T cells. -SCBT Publication Review
Date published: 2015-04-16
Rated 5 out of 5 by LF from Strong band in WB in HeLa whole cell lysateStrong band in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-04-08
Rated 5 out of 5 by QZ from Exceptional immunofluorescence membrane stainingExceptional immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-14
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Annexin-2/ANXA2 抗体 (3D5) is rated 5.0 out of 5 by 7.
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