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Annexin-2/ANXA2 Antibody (3D5) - Western Blotting - Image 379815 — Annexin II 抗体 (3D5): sc-47696. MCF7 (A), HUV-EC-C (B), WI-38 (C) 和 A549 (D) 全细胞裂解液中 Annexin II 表达的 Western 印迹分析.

Annexin II 抗体 (3D5): sc-47696. MCF7 (A), HUV-EC-C (B), WI-38 (C) 和 A549 (D) 全细胞裂解液中 Annexin II 表达的 Western 印迹分析.

Annexin-2/ANXA2 抗体 (3D5): sc-47696

5.0(7)

寫評論提問

Annexin-2/ANXA2抗体(3D5)是小鼠单克隆IgG_2a κ, 在16篇文献中引用，规格为200 µg/ml
针对human骨组织中的全天然附件蛋白
抗-Annexin-2/ANXA2 抗体 (3D5) 推荐用于 WB, IP 和 IF，检测mouse, rat 和human 来源的 Annexin II
抗Annexin-2/ANXA2抗体（3D5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
关于如何获取Annexin-2/ANXA2 (3D5): sc-47696的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgG Fc BP-HRP是Annexin-2/ANXA2 Antibody (3D5) 适用于 WB 应用。的首选辅助检测试剂。该试剂现与Annexin-2/ANXA2 Antibody (3D5) 搭配使用（请参阅下面的订购信息）。

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描述

基因信息

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研究信息

関連項目

额外Annexin抗体，包括Annexin A10, Annexin A13, Annexin A9, Annexin I, Annexin II, Annexin III, Annexin IV, Annexin V, Annexin VI, Annexin VII, Annexin VIII和Annexin XI

Annexin II抗体（3D5）是一种小鼠单克隆IgG2a kappa轻链抗体，针对人类骨骼组织中的天然Annexin蛋白，能够通过蛋白质印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）检测小鼠、大鼠和人类来源的Annexin II。Annexin II，也称为Annexin A2、Calpactin II、Chromobindin-8、Lipocortin II或p36，是一种钙依赖性磷脂结合蛋白，在各种细胞过程中发挥着关键作用。Annexin II主要位于细胞质和细胞表面，可调节与膜相关的事件，如内吞作用、外排作用和细胞粘附。表面表达的Annexin II可作为纤溶酶原和纤溶酶原激活剂（tPA）的受体，促进纤溶酶的产生，并在纤维蛋白溶解和血液凝固中发挥关键作用。Annexin II可以以单体形式存在，也可以与S100A10（p11）形成异四聚体复合物，后者被称为钙蛋白激酶I，参与信号转导通路。Annexin II也是蛋白激酶C（PKC）和Src家族激酶的底物，表明它参与了磷酸化依赖性信号传导机制。抗Annexin II抗体（3D5）使研究人员能够研究Annexin II在病理条件下的作用，例如癌症进展、炎症和凝血障碍。Annexin II (3D5) 抗体有未结合和多种结合形式，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 以及各种 Alexa Fluor® 结合物，为不同的实验应用提供了多功能性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor^® 是Molecular Probes Inc., OR., USA的商标

LI-COR^®和 Odyssey^® 是LI-COR Biosciences的注册商标

订购信息

产品名称	产品编号	规格	价格	数量		收藏夹
Annexin-2/ANXA2 抗体 (3D5)	sc-47696	200 µg/ml	RMB2377.00
Annexin-2/ANXA2 (3D5): m-IgG Fc BP-HRP 套装	sc-525551	200 µg Ab; 10 µg BP	RMB2662.00
Annexin-2/ANXA2 抗体 (3D5) AC	sc-47696 AC	500 µg/ml, 25% agarose	RMB3129.00
Annexin-2/ANXA2 抗体 (3D5) HRP	sc-47696 HRP	200 µg/ml	RMB2377.00
Annexin-2/ANXA2 抗体 (3D5) FITC	sc-47696 FITC	200 µg/ml	RMB2482.00
Annexin-2/ANXA2 抗体 (3D5) PE	sc-47696 PE	200 µg/ml	RMB2580.00
Annexin-2/ANXA2 抗体 (3D5) Alexa Fluor^® 488	sc-47696 AF488	200 µg/ml	RMB2685.00
Annexin-2/ANXA2 抗体 (3D5) Alexa Fluor^® 546	sc-47696 AF546	200 µg/ml	RMB2685.00
Annexin-2/ANXA2 抗体 (3D5) Alexa Fluor^® 594	sc-47696 AF594	200 µg/ml	RMB2685.00
Annexin-2/ANXA2 抗体 (3D5) Alexa Fluor^® 647	sc-47696 AF647	200 µg/ml	RMB2685.00
Annexin-2/ANXA2 抗体 (3D5) Alexa Fluor^® 680	sc-47696 AF680	200 µg/ml	RMB2685.00
Annexin-2/ANXA2 抗体 (3D5) Alexa Fluor^® 790	sc-47696 AF790	200 µg/ml	RMB2685.00

What about cross-reactivity with annexinA1 and annexin A3?

Asked by: mtsanna

Thank you for your question. This antibody is specific for Annexin II and should not pick up Annexin 1 and Annexin III. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com

Answered by: Tech Service

Date published: 2017-11-18

What is the recommended fixation method for immunofluorescence staining with Annexin II (3D5): sc-47696 monoclonal antibody?

Asked by: TinTin

Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining

Answered by: Technical Support

Date published: 2017-02-24

Rated 5 out of 5 by TimS from Works GreatAll of the Santa Cruz Annexin II antibodies have been good experiences for us.

Date published: 2019-02-06

Rated 5 out of 5 by sticky from Specific staining (cell membranes)Specific staining (cell membranes). Excellent antibody for IHC. Reagents for blocking steps & seconary antibody from Dako

Date published: 2017-02-14

Rated 5 out of 5 by FL from Robust band seen in WB in KRobust band seen in WB in K-562 whole cell lysate. -SCBT QC

Date published: 2015-05-18

Rated 5 out of 5 by MS from Good specificity in WB in human Annexin IIGood specificity in WB in human Annexin II transfected 293 whole cell lysate. -SCBT QC

Date published: 2015-04-28

Rated 5 out of 5 by FL from Published data for WB and IF in HEK293T cellsPublished data for WB and IF in HEK293T cells. -SCBT Publication Review

Date published: 2015-04-16

Rated 5 out of 5 by LF from Strong band in WB in HeLa whole cell lysateStrong band in WB in HeLa whole cell lysate. -SCBT QC

Date published: 2015-04-08

Rated 5 out of 5 by QZ from Exceptional immunofluorescence membrane stainingExceptional immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC

Date published: 2015-03-14

Annexin-2/ANXA2 抗体 (3D5) is rated 5.0 out of 5 by 7.

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