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ANAPC2 Antibody (8G2) - Western Blotting - Image 349569
ANAPC2 抗体 (8G2): sc-517022. 人重组 ANAPC2 融合蛋白的 Western 印迹分析.
ANAPC2 Antibody (8G2) - Western Blotting - Image 349569
ANAPC2 抗体 (8G2): sc-517022. 人重组 ANAPC2 融合蛋白的 Western 印迹分析.
ANAPC2 抗体 (8G2): sc-517022. 人重组 ANAPC2 融合蛋白的 Western 印迹分析.

ANAPC2 抗体 (8G2): sc-517022

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说明书
  • ANAPC2 抗体 8G2 是小鼠单克隆 IgG1 κ，ANAPC2抗体, 在1篇文献中引用，规格为100 µg/ml
  • 与氨基酸716-822结合，代表human起源的部分长度ANAPC2
  • 推荐用于 human 来源的ANAPC2 WB, IP 和 ELISA检测
  • 目前，我们还没有完成ANAPC2 抗体 (8G2)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

ANAPC2抗体（8G2）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）和酶联免疫吸附测定（ELISA）检测人类源性ANAPC2蛋白。抗ANAPC2抗体（8G2）以非结合形式提供。ANAPC2在细胞周期正常进展中起着至关重要的作用。该复合物由十多个亚基组成，在细胞有丝分裂的中期到后期过渡期间促进姐妹染色单体分离。APC通过泛素化有丝分裂周期蛋白和其他调节蛋白来实现这一目标，这些蛋白必须降解才能进行细胞分裂。APC的激活受到多种因素的严格调控，包括Cdc2/cyclin B和Polo样激酶（Plk）等蛋白激酶的磷酸化，以及与p55 CDC、E-cadherin和MAD2等调节蛋白的相互作用。值得注意的是，p55 CDC和E-cadherin会增强APC对细胞周期蛋白的泛素化活性，而MAD2则通过形成三元复合物来抑制APC的激活。ANAPC2（也称为APC2）与Ubc4或UbcH10和APC11形成异二聚体复合物，负责对人类securin和cyclin B1等关键蛋白进行泛素化。ANAPC2的C端cullin同源结构域与APC11和UBE2C结合，进一步强调了ANAPC2在细胞周期调节中的不可或缺的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ANAPC2 抗体 (8G2) 参考文献:

  1. 无相促进复合体的亚基和底物。  |  Peters, JM. 1999. Exp Cell Res. 248: 339-49. PMID: 10222126
  2. 无性繁殖促进复合体对有丝分裂转变的控制  |  Fang, G., et al. 1999. Philos Trans R Soc Lond B Biol Sci. 354: 1583-90. PMID: 10582244
  3. 无相促进复合体：新的亚基和调节器。  |  Page, AM. and Hieter, P. 1999. Annu Rev Biochem. 68: 583-609. PMID: 10872461
  4. APC2 Cullin 蛋白和 APC11 RING 蛋白组成了无性繁殖促进复合体的最小泛素连接酶模块。  |  Tang, Z., et al. 2001. Mol Biol Cell. 12: 3839-51. PMID: 11739784
  5. cyclosome/APC 的细胞周期蛋白-泛素连接酶活性是由蛋白激酶 Cdk1-cyclin B 和 Plk 共同激活的。  |  Golan, A., et al. 2002. J Biol Chem. 277: 15552-7. PMID: 11859075
  6. 减数分裂特异性蛋白激酶 Ime2 对 APC 介导的蛋白水解的抑制。  |  Bolte, M., et al. 2002. Proc Natl Acad Sci U S A. 99: 4385-90. PMID: 11917129
  7. 无相促进复合体的一个亚基是哺乳动物细胞中与中心粒相关的蛋白质。  |  Jörgensen, PM., et al. 1998. Mol Cell Biol. 18: 468-76. PMID: 9418894

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产品名称产品编号规格价格数量收藏夹

ANAPC2 抗体 (8G2)

sc-517022
100 µg/ml
RMB2377.00

For Western Blot, is it recommended to use denatured or non-denatured conditions with ANAPC2 (8G2): sc-517022 antibody?

Asked by: Dr Ninau Qelp
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 5 out of 5 by from Good for Western blotAntibody works for IP Western blot in published research with stable OLC1-overexpressing cell line KYSE150/GFP-OLC1; IP with anti-OLC1, Western blot with anti-ANAPC2. -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from Highly recommend for ANAPC2 analysisWorks well in Western Blot, used in WB on human peripheral blood leukocytes, Molecular Weight is at the right position.
Date published: 2017-05-28
Rated 5 out of 5 by from specific stainingspecific staining of paraffin-embedded human skin tissue showing cytoplasmic staining of epidermal cells in IF.
Date published: 2017-04-15
Rated 5 out of 5 by from best antibodyThe best antibody for western blot of human kidney tumor, strong bands at 1:1000 dilution.
Date published: 2017-04-15
Rated 5 out of 5 by from the advantages of itMost of the Escherichia coli expression and recombinant protein or polypeptide as immunogen, the full-length protein MaxPab antibody in eukaryotic expression as the immunogen, the immunogen is using CellFree Science Co Ltd. (CFS) no recombinant protein expressed cells of wheat germ protein synthesis system patents, has good solubility and. Thus, some post-translational modification of functional proteins. Therefore, the antibody prepared by the immunogen is more specific to endogenous proteins.
Date published: 2017-01-19
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ANAPC2 抗体 (8G2) is rated 5.0 out of 5 by 5.
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