Date published: 2025-9-6

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AMPKγ2 抗体 (F-2): sc-398804

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说明书
  • AMPK gamma 2 抗体 F-2 是小鼠单克隆 IgG1 (kappa轻链),AMPKγ2抗体,规格为200 µg/ml
  • 特异性抗原位于human物种的AMPKγ2的N-terminus附近的氨基酸36-65之间
  • AMPK gamma 2 抗体 (F-2) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 AMPKγ2
  • 抗AMPK gamma 2抗体(F-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取AMPKγ2 (F-2): sc-398804免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是AMPKγ2 Antibody (F-2) for WB applications. 的首选辅助检测试剂,这些试剂现与AMPKγ2 Antibody (F-2) 打包提供(请参阅下面的订购信息)。

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    関連項目

    AMPKγ2抗体(F-2)是一种IgG1 κ小鼠单克隆AMPK gamma 2抗体(也称为AMPKγ2抗体),可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附试验(ELISA)检测小鼠、大鼠和人类来源的AMPK gamma 2蛋白。AMPKγ2抗体(F-2)既有非偶联形式的抗AMPK gamma 2抗体,也有多种偶联形式的抗AMPK gamma 2抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。AMPK(5'-AMP激活的蛋白激酶)是一个异源三聚体复合物,由催化性的α亚基和调节性的β和γ亚基组成。它通过关闭消耗ATP的生物合成途径,保护细胞免受导致ATP耗竭的压力。AMPK在高AMP和低ATP的作用下被激活,其机制涉及变构调节、上游蛋白激酶(称为AMPK激酶)促进磷酸化和抑制去磷酸化。活化的AMPK可以在体内磷酸化和调节羟甲基戊二酰辅酶A还原酶和乙酰辅酶A羧化酶,它们分别是甾醇合成和脂肪酸合成的关键调节酶。人类AMPKα1和AMPKα2基因分别编码548个氨基酸和552个氨基酸的蛋白质。人类AMPKβ1编码一个271个氨基酸的蛋白质,人类AMPKβ2编码一个272个氨基酸的蛋白质。人类AMPKγ1基因编码一个331个氨基酸的蛋白质。人类AMPKγ2和AMPKγ3分别是569和492个氨基酸的蛋白质,它们包含独特的N端结构域,可能直接参与AMPK复合物内AMP的结合。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    AMPKγ2 抗体 (F-2) 参考文献:

    1. AMP 活化蛋白激酶 gamma 亚基同工酶的特征及其在 AMP 结合中的作用。  |  Cheung, PC., et al. 2000. Biochem J. 346 Pt 3: 659-69. PMID: 10698692
    2. 糖原贮积病是PRKAG2心脏综合征中的一种统一疾病机制。  |  Gollob, MH. 2003. Biochem Soc Trans. 31: 228-31. PMID: 12546691
    3. 靶向反义长链非编码RNA PRKAG2-AS1作为治疗靶点抑制肝细胞癌细胞的恶性行为。  |  Ou, Y., et al. 2021. Front Med (Lausanne). 8: 649279. PMID: 33928106
    4. PRKAG2综合征的长期心脏并发症。  |  Magalhães, LP., et al. 2022. Arq Bras Cardiol. 118: 106-109. PMID: 35195217
    5. PRKAG2的CBS与非CBS结构域变体在心律失常和心肌病中的分子功能争议:病例报告和文献综述。  |  Gong, X., et al. 2022. Mol Genet Genomic Med. 10: e1962. PMID: 35588295
    6. 鉴定导致PRKAG2心肌病的错义变体的致病效应。  |  Komurcu-Bayrak, E., et al. 2022. Arch Biochem Biophys. 727: 109340. PMID: 35787834
    7. 家族内表型变异与 PRKAG2 突变相关的家族病例报告和文献综述。  |  Marcu, AS., et al. 2022. Life (Basel). 12: PMID: 36556501
    8. PRKAG2-AS的异常表达通过与PPARG相互作用调节PRKAG2转录物,导致心肌细胞功能障碍。  |  Song, XW., et al. 2023. Clin Epigenetics. 15: 178. PMID: 37932845
    9. 哺乳动物 AMP 激活蛋白激酶亚家族。  |  Stapleton, D., et al. 1996. J Biol Chem. 271: 611-4. PMID: 8557660
    10. AMP-活化蛋白激酶--哺乳动物细胞的燃料调节器?  |  Hardie, DG. and Carling, D. 1997. Eur J Biochem. 246: 259-73. PMID: 9208914
    11. AMP激活的蛋白激酶同工酶家族:亚基结构和染色体位置。  |  Stapleton, D., et al. 1997. FEBS Lett. 409: 452-6. PMID: 9224708
    12. 鉴定在骨骼肌中高表达的新型 AMP 激活蛋白激酶 beta 亚基同工酶。  |  Thornton, C., et al. 1998. J Biol Chem. 273: 12443-50. PMID: 9575201

    订购信息

    产品名称产品编号规格价格数量收藏夹

    AMPKγ2 抗体 (F-2)

    sc-398804
    200 µg/ml
    RMB2377.00

    AMPKγ2 (F-2): m-IgG Fc BP-HRP 套装

    sc-527812
    200 µg Ab; 10 µg BP
    RMB2662.00

    AMPKγ2 (F-2): m-IgG1 BP-HRP 套装

    sc-533185
    200 µg Ab; 20 µg BP
    RMB2662.00

    AMPKγ2 抗体 (F-2) AC

    sc-398804 AC
    500 µg/ml, 25% agarose
    RMB3129.00

    AMPKγ2 抗体 (F-2) HRP

    sc-398804 HRP
    200 µg/ml
    RMB2377.00

    AMPKγ2 抗体 (F-2) FITC

    sc-398804 FITC
    200 µg/ml
    RMB2482.00

    AMPKγ2 抗体 (F-2) PE

    sc-398804 PE
    200 µg/ml
    RMB2580.00

    AMPKγ2 抗体 (F-2) Alexa Fluor® 488

    sc-398804 AF488
    200 µg/ml
    RMB2685.00

    AMPKγ2 抗体 (F-2) Alexa Fluor® 546

    sc-398804 AF546
    200 µg/ml
    RMB2685.00

    AMPKγ2 抗体 (F-2) Alexa Fluor® 594

    sc-398804 AF594
    200 µg/ml
    RMB2685.00

    AMPKγ2 抗体 (F-2) Alexa Fluor® 647

    sc-398804 AF647
    200 µg/ml
    RMB2685.00

    AMPKγ2 抗体 (F-2) Alexa Fluor® 680

    sc-398804 AF680
    200 µg/ml
    RMB2685.00

    AMPKγ2 抗体 (F-2) Alexa Fluor® 790

    sc-398804 AF790
    200 µg/ml
    RMB2685.00

    AMPKγ2 (F-2) 中和勝肽

    sc-398804 P
    100 µg/0.5 ml
    RMB511.00

    What application is the blocking peptide sc-398804 P appropriate for?

    Asked by: TinTin
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 4 out of 5 by from We can get the band.We can see the band, but high background. Application: WB Sample: rat microglia culture
    Date published: 2020-08-25
    Rated 5 out of 5 by from Great featuresI applied an sample a mouth ago, and I did Westen blot (dilution 1:100), the result have nonspecific bands
    Date published: 2017-08-17
    Rated 5 out of 5 by from work well in WBUsed the new antibody,got the good result,while it is signal,clean bands,and it is evryone‘s desires
    Date published: 2017-01-10
    Rated 5 out of 5 by from Strong band in WB in mouse AMPK 2 transfectedStrong band in WB in mouse AMPK 2 transfected whole cell lysate. -SCBT QC
    Date published: 2013-05-12
    Rated 5 out of 5 by from Nice Western blot data of AMPK 2 expression in nonNice Western blot data of AMPK 2 expression in non-transfected and mouse AMPK 2 transfected whole cell lysates. -SCBT QC
    Date published: 2013-05-12
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    AMPKγ2 抗体 (F-2) is rated 4.8 out of 5 by 5.
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