Date published: 2025-9-7

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AMPKβ1 抗体 (Z14): sc-100357

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说明书
  • AMPK beta 1 抗体 Z14 是小鼠单克隆 IgG2a κ,AMPKβ1抗体, 在5篇文献中引用,规格为100 µg/ml
  • 免疫human物种的重组AMPKβ1
  • 推荐用于 mouse, rat 和 human 来源的AMPKβ1 WB, IP, IF 和 ELISA检测
  • 关于如何获取AMPKβ1 (Z14): sc-100357免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2a BP-HRP">m-IgG2a BP-HRP是AMPKβ1 Antibody (Z14) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与AMPKβ1 Antibody (Z14) 打包提供(请参阅下面的订购信息)。

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AMPKβ1抗体(Z14)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类的AMPKβ1。抗AMPKβ1抗体(Z14)以非结合形式提供。AMPK(5'-AMP激活蛋白激酶)复合物由催化α亚基和调节β、γ亚基组成,在细胞能量稳态中起着至关重要的作用。AMPKβ1主要位于细胞质中,对于感知细胞能量水平至关重要,而细胞能量水平对于维持代谢平衡至关重要。当细胞能量不足时,AMP增加和ATP减少会激活AMPKβ1,导致关键代谢酶的磷酸化,从而抑制能量消耗过程并促进能量产生途径。这种调节机制在代谢压力条件下尤为重要,例如在运动或营养缺乏期间,因为AMPKβ1有助于恢复能量平衡并促进细胞存活。人类AMPKβ1基因编码一种271个氨基酸的蛋白质,它与α和γ亚基相互作用,形成功能性AMPK复合物,从而影响各种代谢途径和细胞反应。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

AMPKβ1 抗体 (Z14) 参考文献:

  1. AMP 活化蛋白激酶 gamma 亚基同工酶的特征及其在 AMP 结合中的作用。  |  Cheung, PC., et al. 2000. Biochem J. 346 Pt 3: 659-69. PMID: 10698692
  2. 水杨酸酯(水杨酸盐)独立于 AMPK-β1 解偶联线粒体、改善葡萄糖稳态并降低肝脏脂质。  |  Smith, BK., et al. 2016. Diabetes. 65: 3352-3361. PMID: 27554471
  3. 激活 AMPK β1 可抑制抗精神病药物诱发的小鼠高血糖。  |  Shamshoum, H., et al. 2019. FASEB J. 33: 14010-14021. PMID: 31581839
  4. 通过 AMPKβ1 减少小鼠动脉粥样硬化。  |  Day, EA., et al. 2021. Mol Metab. 53: 101321. PMID: 34425254
  5. 昼夜节律通过 MTR1/AMPKβ1/BMAL1 信号轴调节软骨内骨形成。  |  Yu, S., et al. 2022. Cell Death Differ. 29: 874-887. PMID: 35094018
  6. AMPKβ1 的磷酸化对于增加自噬和维持线粒体对脂肪酸的平衡至关重要。  |  Desjardins, EM., et al. 2022. Proc Natl Acad Sci U S A. 119: e2119824119. PMID: 36409897
  7. 阻断 AMPK β1 的肉豆蔻酰化可增强 AMPK 的活性,保护小鼠免受高脂饮食引起的肥胖和肝脏脂肪变性的影响。  |  Neopane, K., et al. 2022. Cell Rep. 41: 111862. PMID: 36543129
  8. PF-06409577 激活巨噬细胞 AMPK β1 可减少小鼠的炎症反应、胆固醇合成和动脉粥样硬化。  |  Day, EA., et al. 2023. iScience. 26: 108269. PMID: 38026185
  9. 哺乳动物 AMP 激活蛋白激酶亚家族。  |  Stapleton, D., et al. 1996. J Biol Chem. 271: 611-4. PMID: 8557660
  10. AMP-活化蛋白激酶--哺乳动物细胞的燃料调节器?  |  Hardie, DG. and Carling, D. 1997. Eur J Biochem. 246: 259-73. PMID: 9208914
  11. AMP激活的蛋白激酶同工酶家族:亚基结构和染色体位置。  |  Stapleton, D., et al. 1997. FEBS Lett. 409: 452-6. PMID: 9224708
  12. 鉴定在骨骼肌中高表达的新型 AMP 激活蛋白激酶 beta 亚基同工酶。  |  Thornton, C., et al. 1998. J Biol Chem. 273: 12443-50. PMID: 9575201

订购信息

产品名称产品编号规格价格数量收藏夹

AMPKβ1 抗体 (Z14)

sc-100357
100 µg/ml
$333.00

AMPKβ1 (Z14): m-IgG2a BP-HRP 套装

sc-546014
100 µg Ab; 10 µg BP
$354.00

I am using AMPKβ1 (Z14): sc-100357 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Cweed
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Good Antibody for WBWe can get clear band. Application: WB Sample: rat microglia culture
Date published: 2020-08-11
Rated 5 out of 5 by from good productThis antibody works pretty good for WB experiments.
Date published: 2018-09-11
Rated 4 out of 5 by from WB AMPK β1 (Z-14)One unique strong and clear band at 38 kDa. 40 ug of whole cell extract from 12 month old murine hepatocellular carcinoma (2) tumoral tissue (1) adjacent non tumoral tissue. Dilution 1/1000.
Date published: 2017-10-13
Rated 5 out of 5 by from Great featuresI applied a sample one month ago and I did Westen Blotting (dilution 1:100) at white adipose tissue of mouse, the results is excellent.
Date published: 2017-09-20
Rated 5 out of 5 by from Strong band in WB in HeLa whole cell lysateStrong band in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-05-03
Rated 5 out of 5 by from Clear band in WB in lambda protein phosphataseClear band in WB in lambda protein phosphatase treated differentiated C2C12 whole cell lysate. -SCBT QC
Date published: 2015-04-25
Rated 5 out of 5 by from Produced excellent immunofluorescence nuclearProduced excellent immunofluorescence nuclear and cytoplasmic staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-01-25
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AMPKβ1 抗体 (Z14) is rated 4.9 out of 5 by 7.
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