Date published: 2026-10-24

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α-actinin Antibody (B-12) - Western Blotting - Image 353255
α-actinin 抗体 (B-12): sc-166524. MCF7 (A), A-673 (B), SJRH30 (C), K-562 (D), NIH/3T3 (E) 和 PC-12 (F) 全细胞裂解液中 α-actinin 表达的 Western 印迹分析.
α-actinin 抗体 (B-12): sc-166524. MCF7 (A), A-673 (B), SJRH30 (C), K-562 (D), NIH/3T3 (E) 和 PC-12 (F) 全细胞裂解液中 α-actinin 表达的 Western 印迹分析.

α-actinin 抗体 (B-12): sc-166524

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寫評論提問
说明书
  • α-actinin抗体(B-12)是小鼠单克隆IgG2a κ, 在24篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的α-actinin的C-terminus的氨基酸872-891之间
  • 推荐用于 mouse, rat 和 human 来源的α-actinin-1, α-actinin-2 and α-actinin-4 WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 α-actinin (H-2): sc-17829，可查看有标记的 α-actinin 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是α-actinin Antibody (B-12) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与α-actinin Antibody (B-12) 打包提供（请参阅下面的订购信息）。
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関連項目

附加α-actinin抗体，包括α-actinin-1和α-actinin-4

血影蛋白基因家族编码多种细胞骨架蛋白，包括血影蛋白、肌营养不良蛋白和α-肌动蛋白。有四种组织特异性α-肌动蛋白，即α-肌动蛋白-1、α-肌动蛋白-2、α-肌动蛋白-3和α-肌动蛋白-4，它们定位于肌肉和非肌肉细胞，包括骨骼肌、心肌和平滑肌细胞，以及细胞骨架内。每个α-肌动蛋白蛋白含有一个肌动蛋白结合结构域、两个钙调素同源结构域、两个EF-手结构域和四个血影蛋白重复序列，它们通过这些结构域作为捆绑蛋白发挥作用，可以交联F-肌动蛋白，从而将肌动蛋白锚定到各种细胞内结构。编码α-肌动蛋白-4的基因缺陷是局灶节段性肾小球硬化1（FSGS1）的原因，FSGS1是一种常见的肾脏病变，其特征是肾功能下降，最终导致肾功能衰竭。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

α-actinin 抗体 (B-12) 参考文献:

  1. α-肌动蛋白的分子进化与结构。  |  Virel, A. and Backman, L. 2004. Mol Biol Evol. 21: 1024-31. PMID: 15014165
  2. zyxin与α-肌动蛋白之间的相互作用。  |  Crawford, AW., et al. 1992. J Cell Biol. 116: 1381-93. PMID: 1541635
  3. α-肌动蛋白与神经调节素在神经调节素刺激的乙酰胆碱受体聚集中相互作用。  |  Dobbins, GC., et al. 2008. Mol Brain. 1: 18. PMID: 19055765
  4. 厚膜菌α-肌动蛋白的表征。  |  Cotado-Sampayo, M., et al. 2011. Curr Microbiol. 63: 100-5. PMID: 21598047
  5. 帕拉丁和α-肌动蛋白之间的相互作用的结构特征。  |  Beck, MR., et al. 2011. J Mol Biol. 413: 712-25. PMID: 21925511
  6. α-肌动蛋白激活的分子轨迹。  |  Shams, H., et al. 2012. Biophys J. 103: 2050-9. PMID: 23200039
  7. 微丝蛋白与α-肌动蛋白的特异性相互作用。  |  Wachsstock, DH., et al. 1987. Biochem Biophys Res Commun. 146: 554-60. PMID: 3113423
  8. α-肌动蛋白交联可避免应力纤维中肌球蛋白力传递的粘性耗散。  |  Katsuta, H., et al. 2023. iScience. 26: 106090. PMID: 36852278
  9. 心肌细胞外部机械卸载激活α-肌动蛋白的修饰不同于肌节源性卸载。  |  Solís, C., et al. 2023. FEBS J. 290: 5322-5339. PMID: 37551968
  10. PKN与α-肌动蛋白的相互作用。  |  Mukai, H., et al. 1997. J Biol Chem. 272: 4740-6. PMID: 9030526

订购信息

产品名称产品编号规格价格数量收藏夹

α-actinin 抗体 (B-12)

sc-166524
200 µg/ml
CNY2422.00

α-actinin (B-12): m-IgG Fc BP-HRP 套装

sc-540116
200 µg Ab; 10 µg BP
CNY2715.00

α-actinin (B-12): m-IgG2a BP-HRP 套装

sc-546854
200 µg Ab; 10 µg BP
CNY2715.00

α-actinin (B-12) 中和勝肽

sc-166524 P
100 µg/0.5 ml
CNY519.00

Could this antibody be used for FlowCytometry? Thanks!

Asked by: sme1203
Thank you for your question. We have not tested this antibody, α-actinin (B-12): sc-166524, for flow cytometry (FCM), so it is not a recommended application at this time.
Answered by: Technical Support
Date published: 2017-07-25

What application is the blocking peptide sc-166524 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by Trav11 from Great for IHC(P)The alpha-actinin Antibody (B-12): sc-166524 works very well for IHC applications when using formalin fixed, paraffin-embedded tissue sections, showing strong staining pattern localized to the cytoplasm. This antibody has also been cited several times for further verification.
Date published: 2017-01-30
Rated 5 out of 5 by JM from Strong signal in WB in humanStrong signal in WB in human -actinin-1 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-05-21
Rated 5 out of 5 by HC from Published data for WB in human primary WM115Published data for WB in human primary WM115 and metastatic WM266-4 melanoma cell lines. -SCBT Publication Review
Date published: 2015-05-21
Rated 5 out of 5 by LM from Published data for WB in human colon adenocarcinomaPublished data for WB in human colon adenocarcinoma cell lines HT-29, HCT116 and SW480. -SCBT Publication Review
Date published: 2015-05-10
Rated 5 out of 5 by JM from Published data for WB in human HaCaT cellsPublished data for WB in human HaCaT cells. -SCBT Publication Review
Date published: 2015-05-03
Rated 5 out of 5 by TO from Antibody worked well for WB in L8 and HeLaAntibody worked well for WB in L8 and HeLa whole cell lysates. -SCBT QC
Date published: 2013-07-18
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α-actinin 抗体 (B-12) is rated 5.0 out of 5 by 6.
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