Date published: 2025-9-8
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α-actinin Antibody (B-12) - Western Blotting - Image 353255
α-actinin 抗体 (B-12): sc-166524. MCF7 (A), A-673 (B), SJRH30 (C), K-562 (D), NIH/3T3 (E) 和 PC-12 (F) 全细胞裂解液中 α-actinin 表达的 Western 印迹分析.
α-actinin 抗体 (B-12): sc-166524. MCF7 (A), A-673 (B), SJRH30 (C), K-562 (D), NIH/3T3 (E) 和 PC-12 (F) 全细胞裂解液中 α-actinin 表达的 Western 印迹分析.

α-actinin 抗体 (B-12): sc-166524

5.0(6)
寫評論提問

说明书
  • α-actinin抗体(B-12)是小鼠单克隆IgG2a κ, 在24篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的α-actinin的C-terminus的氨基酸872-891之间
  • 推荐用于 mouse, rat 和 human 来源的α-actinin-1, α-actinin-2 and α-actinin-4 WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 α-actinin (H-2): sc-17829，可查看有标记的 α-actinin 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 α-actinin 抗体 (B-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与α-actinin 抗体 (B-12) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

附加α-actinin抗体，包括α-actinin-1和α-actinin-4

血影蛋白基因家族编码多种细胞骨架蛋白，包括血影蛋白、肌营养不良蛋白和α-肌动蛋白。有四种组织特异性α-肌动蛋白，即α-肌动蛋白-1、α-肌动蛋白-2、α-肌动蛋白-3和α-肌动蛋白-4，它们定位于肌肉和非肌肉细胞，包括骨骼肌、心肌和平滑肌细胞，以及细胞骨架内。每个α-肌动蛋白蛋白含有一个肌动蛋白结合结构域、两个钙调素同源结构域、两个EF-手结构域和四个血影蛋白重复序列，它们通过这些结构域作为捆绑蛋白发挥作用，可以交联F-肌动蛋白，从而将肌动蛋白锚定到各种细胞内结构。编码α-肌动蛋白-4的基因缺陷是局灶节段性肾小球硬化1（FSGS1）的原因，FSGS1是一种常见的肾脏病变，其特征是肾功能下降，最终导致肾功能衰竭。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

α-actinin 抗体 (B-12)

sc-166524
200 µg/ml
RMB2377.00

α-actinin (B-12): m-IgG Fc BP-HRP 套装

sc-540116
200 µg Ab; 10 µg BP
RMB2662.00

α-actinin (B-12): m-IgG2a BP-HRP 套装

sc-546854
200 µg Ab; 10 µg BP
RMB2662.00

α-actinin (B-12) 中和勝肽

sc-166524 P
100 µg/0.5 ml
RMB511.00

Could this antibody be used for FlowCytometry? Thanks!

Asked by: sme1203
Thank you for your question. We have not tested this antibody, α-actinin (B-12): sc-166524, for flow cytometry (FCM), so it is not a recommended application at this time.
Answered by: Technical Support
Date published: 2017-07-25

What application is the blocking peptide sc-166524 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great for IHC(P)The alpha-actinin Antibody (B-12): sc-166524 works very well for IHC applications when using formalin fixed, paraffin-embedded tissue sections, showing strong staining pattern localized to the cytoplasm. This antibody has also been cited several times for further verification.
Date published: 2017-01-30
Rated 5 out of 5 by from Strong signal in WB in humanStrong signal in WB in human -actinin-1 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-05-21
Rated 5 out of 5 by from Published data for WB in human primary WM115Published data for WB in human primary WM115 and metastatic WM266-4 melanoma cell lines. -SCBT Publication Review
Date published: 2015-05-21
Rated 5 out of 5 by from Published data for WB in human colon adenocarcinomaPublished data for WB in human colon adenocarcinoma cell lines HT-29, HCT116 and SW480. -SCBT Publication Review
Date published: 2015-05-10
Rated 5 out of 5 by from Published data for WB in human HaCaT cellsPublished data for WB in human HaCaT cells. -SCBT Publication Review
Date published: 2015-05-03
Rated 5 out of 5 by from Antibody worked well for WB in L8 and HeLaAntibody worked well for WB in L8 and HeLa whole cell lysates. -SCBT QC
Date published: 2013-07-18
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α-actinin 抗体 (B-12) is rated 5.0 out of 5 by 6.
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